Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity

Erukainure, Ochuko L.; Mesaik, M. Ahmed; Muhammad, Aliyu; Chukwuma, Chika I.; Manhas, Neha; Singh, Parvesh; Aremu, Oluwole Samuel; Islam, Md. Shahidul

doi:10.1016/j.biopha.2016.09.002

Cited by 15 publications

(10 citation statements)

References 25 publications

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“…Thus, indicating a suppressive activity on phagocytic respiratory oxidative burst and may be a good drug candidate in the treatment and management of COVID-19 and its complications. This activity correlates with our previous reports on the ability of extracts and isolated compounds from C. voluble leaves and flower to modulate phagocytic oxidative burst in whole blood, PMNs and macrophages (Erukainure, Hafizur, et al, 2017 ; Erukainure, Mesaik, et al, 2017 ; Erukainure et al, 2016 ).…”

Section: Discussionsupporting

confidence: 91%

“…This has been attributed to their ability to modulate inflammatory cytokines and phagocytic activities of monocytes, neutrophils and macrophages (Erukainure, Mesaik, et al, 2017 ; Harun et al, 2015 ), with their phytochemical constituents playing an influential role. Clerodendrum volubile is among medicinal plants reported for their immunomodulatory activities (Erukainure, Hafizur, et al, 2017 ; Erukainure, Zaruwa, et al, 2017 ; Erukainure et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Its antioxidant activities have been reported in diabetic rats, and cancerous cells which correlates with their antidiabetic and anticancer activities (Erukainure, Ashraf, et al, 2018; Erukainure, Hafizur, et al, 2017 ; 2018 ). The flowers as well as protocatechuic acid and pectolinarigenin isolated from the leaves have been reported for their ability to modulate phagocytic oxidative burst in isolated neutrophils and macrophages which demonstrates their immunomodulatory activities (Erukainure, Hafizur, et al, 2017 ; Erukainure, Mesaik, et al, 2017 ; Erukainure et al, 2016 ). The dichloromethane (DCM) fraction of the leaves have been reported for its cytotoxic effect on human embryonic kidney (HEK293) cells (Erukainure, Narainpersad, et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Targeting the initiation and termination codons of SARS-CoV-2 spike protein as possible therapy against COVID-19: the role of novel harpagide 5-O-β-D-glucopyranoside from Clerodendrum volubile P Beauv. (Labiatae)

Erukainure

Atolanı

Muhammad

et al. 2020

Journal of Biomolecular Structure and Dynamics

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The global spread of the coronavirus infections disease − 2019 (COVID-19) and the search for new drugs from natural products particularly from plants are receiving much attention recently. In this study, the therapeutic potential of a new iridoid glycoside isolated from the leaves of Clerodendrum volubile against COVID-19 was investigated. Harpagide 5-O-β-D-glucopyranoside (HG) was isolated, characterised and investigated for its druglikeness, optimized geometry, and pharmacokinetics properties. Its immunomodulatory was determined by chemiluminescence assay using polymorphonuclear neutrophils (PMNs) in addition to T-cell proliferation assay. In silico analysis was used in determining its molecular interaction with severe acute respiratory syndrome coronavirus-2 (SARS-COV-2). HG displayed potent druglikeness properties, with no inhibitory effect on cytochrome P 450 (1A2, 2C19, 2C9, 2D6 and 3A4) and a predicted LD 50 of 2000 mg/kg. Its 1 H-NMR chemical shifts showed a little deviation of 0.01 and 0.11 ppm for H-4 and H-9, respectively. HG significantly suppressed oxidative bursts in PMNs, while concomitantly inhibiting T-cell proliferation. It also displayed a very strong binding affinity with the translation initiation and termination sequence sites of spike (S) protein mRNA of SARS-COV-2, its gene product, and host ACE2 receptor. These results suggest the immunomodulatory properties and anti-SARS-COV-2 potentials of HG which can be explored in the treatment and management of COVID-19. Communicated by Ramaswamy H. Sarma

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Targeting the initiation and termination codons of SARS-CoV-2 spike protein as possible therapy against COVID-19: the role of novel harpagide 5-O-β-D-glucopyranoside from Clerodendrum volubile P Beauv. (Labiatae)

Erukainure

Atolanı

Muhammad

et al. 2020

Journal of Biomolecular Structure and Dynamics

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“…The ability of the leaves extracts to inhibit to α-amylase, α-glucosidase and angiotensin-1 converting enzyme has been reported recently ( Adefegha and Oboh, 2016 ). Studies have also demonstrated the ability of the chemical fractions and fatty acids of the flower and stem to modulate phagocytic oxidative burst ( Erukainure et al, 2016a , 2017c ). Despite these studies, there is still a dearth of information on the effect of the leaves on T2D complications and other compounds that may contribute to its acclaimed antidiabetic properties.…”

Section: Introductionmentioning

confidence: 99%

Suppressive Effects of Clerodendrum volubile P Beauv. [Labiatae] Methanolic Extract and Its Fractions on Type 2 Diabetes and Its Complications

et al. 2018

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Type 2 diabetes is the most prominent of all diabetes types, contributing to global morbidity and mortality. Availability and cost of treatment with little or no side effect especially in developing countries, remains a huge burden. This has led to the search of affordable alternative therapies especially from medicinal plants. In this study, the antidiabetic effect of the methanolic extract, dichloromethane (DCM), butanol (BuOH) and aqueous fractions of Clerodendrum volubile leaves were investigated in type 2 diabetic rats for their effect on glucose homeostasis, serum insulin level and hepatic biomarkers, lipid profile, pancreatic redox balance and Ca2+ levels, and β-cell distribution and function. The DCM was further fractionated to isolate the active compounds, biochanin and 5,7,4′-trimethoxykaempferol. They were investigated for their toxicity and ADMET properties, α-glucosidase and angiotensin I converting enzyme (ACE) inhibitory activities in silico. There were significant (p < 0.05) decrease in blood glucose, cholesterol, LDL-C, vLDL-C, triglyceride, AST and ALT levels in all treated groups, with DCM fraction showing the best activity. All treated rats showed significantly (p < 0.05) improved anti-oxidative activities. Treatment with the DCM fraction led to significant (p < 0.05) increased serum insulin and pancreatic Ca2+ levels, as well as improved β-cell distribution and function. DCM fraction also showed improved glucose tolerance. DCM fraction dose-dependently inhibited ACE activity. The toxicity class of the isolated compounds was predicted to be 5. They were also predicted to be potent inhibitors of cytochrome P (CYPs) 1A2, 2D6 and 3A4. They docked well with α-glucosidase and ACE. These results indicate the therapeutic potential of the plant against type 2 diabetes, with the DCM fraction being the most potent which may be attributed to the isolated flavones. It further suggests antihypertensive potentials of the DCM fraction. However, inhibition of CYPs by the flavones may suggest caution in usage with other prescribed drugs metabolized by these enzymes.

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“…In folklore medicine, the plant is useful for the treatment of several ailments such as diabetes, rheumatism, arthritis, edema and gout [26,27]. The reported pharmacological properties of C. volubile leaf includes anti-inflammatory [27], antioxidants [28,29], antihypertensive [25,30], antidiabetic [31,32], neuroprotective [33], hepatoprotective [34,35] and cancer chemopreventive [36,37] activities. In a recent study by Erukainure et al [38], the antidiabetic effect of acute treatment with the ethyl acetate fraction of C. volubile leaves was investigated and this property was attributed to the protocatechuic acid which was observed to be the active compound of the plant.…”

Section: Introductionmentioning

confidence: 99%

Liver mitochondrial membrane permeability modulation in insulin-resistant, uninephrectomised male rats by Clerodendrum volubile P. Beauv and Manihot esculenta Crantz

et al. 2019

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Background: Non-alcoholic fatty liver disease, which occurs in people who are not alcohol drinkers, describes some of the pathogenic conditions that may be in the least characterized by simple steatosis or can be as serious as non-alcoholic steatohepatitis and cirrhosis. Its mechanistic pathogenesis has been said to arise from insulin resistance and oxidative stress, which may be compounded by obesity. An experimental model showing, systemic insulin resistance, obesity and accumulated hepatic fatty acids was created in adult male rats using high-fat diet manipulation and surgical removal of the left kidney (uninephrectomy). This study sought to identify the impact of these multiple burdens on the liver mitochondrial membrane permeability transition pore opening, and the possible in vitro effects of the extracts of Clerodendrum volubile and Manihot esculenta leaves on the membrane permeabilization. Results: The results indicated that the methanolic extract of Clerodendrum volubile leaf inhibited mitochondrial membrane pore opening in the insulin resistance condition or when it is followed by uni-nephrectomy, while the ethanolic extract of Manihot esculenta leaf does the same in the insulin resistance condition both prior to and following uni-nephrectomy. Conclusion: Since the vegetable extracts were able to abrogate mitochondrial pore opening at low concentrations, the structural integrity of the mitochondria can possibly be restored over time if treated by the vegetable extracts. Research efforts should, therefore, be made to harness the drugability of the bioactives of these vegetables for use in the treatment of non-alcoholic fatty liver disease arising from insulin resistance and renal failure.

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Flowers of Clerodendrum volubile exacerbate immunomodulation by suppressing phagocytic oxidative burst and modulation of COX-2 activity

Cited by 15 publications

References 25 publications

Targeting the initiation and termination codons of SARS-CoV-2 spike protein as possible therapy against COVID-19: the role of novel harpagide 5-O-β-D-glucopyranoside from Clerodendrum volubile P Beauv. (Labiatae)

Targeting the initiation and termination codons of SARS-CoV-2 spike protein as possible therapy against COVID-19: the role of novel harpagide 5-O-β-D-glucopyranoside from Clerodendrum volubile P Beauv. (Labiatae)

Suppressive Effects of Clerodendrum volubile P Beauv. [Labiatae] Methanolic Extract and Its Fractions on Type 2 Diabetes and Its Complications

Liver mitochondrial membrane permeability modulation in insulin-resistant, uninephrectomised male rats by Clerodendrum volubile P. Beauv and Manihot esculenta Crantz

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