Background Doxorubicin (DOX) induces toxicity in many tissues/organs, including the heart, kidney and so on. This study was designed to evaluate the modulatory effects of protocatechuic acid (PCA) against DOX-induced nephrotoxicity in rats. Animals were randomly grouped into five groups. Methods Group 1 served as the normal control (CTR). A single dose of DOX at 20 mg/kg was administered intraperitoneally (i.p.) to animals in Group 2. Groups 3 and 4 were pretreated with PCA for 5 days (doses of 10 and 20 mg/kg body weight, respectively) after which DOX was injected (PCA-10 + DOX and PCA-20 + DOX). Group 5 received PCA only at a dose of 20 mg/kg body weight (PCA-20). Results The results revealed significant elevations (p < 0.05) in malondialdehyde content, expressions of inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX2) in the kidney. Likewise, increased serum levels of creatinine and urea of DOX group were observed. A significant decrease (p < 0.05) in glutathione (GSH) level and antioxidant enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione s- transferase (GST) activities in the kidney were observed compared with the control. Pretreatment with PCA (10 and 20 mg/kg, p.o.) for 5 days prior to the i.p. injection of DOX reduced MDA levels, modulated iNOS and COX2 activities and improved kidney function markers as well as oxidative stress parameters. Findings from the histopathology studies confirms the protective effects of PCA on DOX-induced damage on the kidney cells. Conclusions This study has demonstrated the anti-inflammatory and antioxidative properties of PCA, which could be part of its possible protective mechanisms against nephrotoxicity induced by DOX.
White Butterfly (Clerodendrum volubile) leaf is commonly used in traditional medicine for the management of various diseases including diabetes without the full understanding of the scientific basis for its use. This study sought to evaluate the antihyperglycemic, antihyperlipidemic and antioxidant effect of C. volubile leaves in streptozotocin (STZ)-induced diabetic rats. Aqueous extract of C. volubile was prepared and its effect assessed on relevant enzymes associated with diabetes. Fifty male Wistar rats were randomly separated into 10 groups each containing five rats. The induction of diabetes in rats was by a single intraperitoneal injection of STZ (65 mg/kg body weight) while C. volubile extract was administered orally to diabetic and non-diabetic animals, at the doses of 50, 100 and 200 mg/kg body weight for 14 days. Metformin (100 mg/kg body weight) served as positive control. Clerodendrum volubile extract inhibited α-glucosidase (IC = 0.20 mg/ml) and α-amylase (IC = 0.58 mg/ml). Furthermore, administration of C. volubile extract significantly reduced the elevated plasma glucose level and body weight, improved kidney functions, attenuated oxidative stress by decreasing MDA levels, enhancing superoxide dismutase, catalase and glutathione peroxidase activities, reinstated the lipid profile to nearly normal level and restored pancreatic histological integrity in diabetic rats. The results reveal that C. volubile represents a source of phytochemicals that exerts their antidiabetic effects through the modulation of glycemic and atherogenic indices as well as mitigation of free-radical-mediated damage.
Pumpkin seed has been associated with myriad of medicinal uses in different part of the world. In this study, phenolic composition and Fe 2+ induced thiobarbituric acid reactive species (TBARS) inhibitory ability of methanolic extract from pumpkin seeds in rat's testes homogenates were determined. The extract was prepared with 80% methanol (v/v) and the radicals [(1,1-diphenyl-2 picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)] scavenging, Fe 2+ chelation and ferric reducing abilities of the extract were carried out. The phenolics composition was also investigated using gas chromatography couple with flame ionization detector (GC-FID). The GC analysis revealed the presence of vallinic, coumaric protocatechuic, caffeic, ferulic and sinapinic acids, and apigenin, quercetin, luteolin, kaempferol as the dominant phenolic compounds. The results revealed that the extract inhibited Fe 2+ -induced TBARS, scavenge DPPH radical and chelate Fe 2+ in a dose dependent manner. The extract also scavenged ABTS radical and reduced Fe 2+ to Fe 3+ . Although, the standard used had higher effect compared to the extract, nevertheless, the TBARs inhibitory potential of the extracts clearly gives an insight on the protective potentials against oxidative induce testicular damage that might lead to male infertility if unchecked. These abilities could however be linked to the presence of polyphenolic compounds.
In this study, the phenolic content, characterization and antioxidant properties of aqueous extracts of three varieties of Celosia species were assessed. Celosia laxa had significantly (P < 0.05) higher total phenol and flavonoid contents than Celosia argentea (red variant) and Celosia argentea (green variant). Gallic acid, caffeic acid, quercetin and rutin were found in the three species of Celosia investigated. Rosmarinic acid and phenol glycoside were the two major compounds identified by high‐performance liquid chromatography–diode‐array detector in C. laxa, rutin and epigallocatechin were the prominent phenolics present in C. argentea (green variant) while C. argentea (red variant) had epigallocatechin as the most abundant phenolic compound. The extracts showed strong antioxidant actions as revealed by their 2,2‐diphenyl‐1‐picrylhydrazyl radical, 2,2′‐azinobis‐3‐ethylbenzo‐thiazoline‐6‐sulfonate, nitric oxide radical‐scavenging abilities and reducing power. These findings indicate that the aqueous extracts from Celosia species are potential sources of natural antioxidants. Practical Application Celosia laxa and Celosia argentea (green and red variants) are green leafy vegetables commonly consumed as food and serve as important ingredients in traditional medicine in Africa and Asia. The assessment of the antioxidant properties, phenolic content and composition of the three Celosia species may give insight into their applications in functional foods and nutraceuticals development.
Natural products possessing antioxidant properties play a very crucial role in ameliorating deleterious effects of reactive oxygen species. This study investigated the chemoprotective properties of methanolic extract of Vernonia amygdalina (MEVA) in an experimental model of hepatic oxidative damage induced by 2-acetylaminofluorene (2-AAF). Rats were divided into six groups. Groups 1 and 2 received saline and dimethyl sulfoxide, respectively, and served as controls. Group 3 received MEVA at a dose of 250 mg/kg, while groups 5 and 6 were pretreated for 14 days with MEVA at 250 mg/kg and 500 mg/kg doses before coadministration with 2-AAF at 100 mg/kg for another 7 days. 2-AAF was administered to group 4 for the last 7 days. Animals were killed 24 h after the last administration of 2-AAF. 2-AAF significantly (p < 0.05) induced marked hepatic damage as revealed by increased activities of serum enzymes such as alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ-glutamyl transferase and bilirubin concentration. 2-AAF also elicited decrease in the activities of antioxidant enzymes such as superoxide dismutase, catalase, glutathione-S-transferase, and glutathione peroxidase, depletion of reduced glutathione, and increase in malondialdehyde levels. The activities of glucose-6-phosphatase and 5'-nucleotidase were also depleted. MEVA at 250 mg/kg and 500 mg/kg significantly (p < 0.05) ameliorated the oxidative damage, functional impairments, and histopathological changes associated with 2-AAF toxicity by reducing the activities of serum enzymes, upregulating the antioxidant defense enzymes and glutathione with decrease in malondialdehyde level. In this study, the revealed ameliorative and hepatoprotective effects of MEVA against 2-AAF-induced toxicity may be due to its antioxidant and free-radical scavenging activities, thus suggesting its usefulness as a possible chemoprophylactic agent.
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