Flow injection system for on-line monitoring of fumaric acid in biological processes

Rhee, Jong Il; Sohn, Ok-Jae

doi:10.1016/j.aca.2003.08.029

Cited by 9 publications

(1 citation statement)

References 20 publications

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“…In contrast, on-line monitoring can quickly and accurately indicate not only substrate and product variations but also formation of and changes in intermediates. [11][12][13][14] Accordingly, the on-line procedure displays a significant advantage in product quality control.…”

mentioning

confidence: 99%

Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

Cheng

Chang

2007

ANAL. SCI.

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mentioning

confidence: 99%

Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

Cheng

Chang

2007

ANAL. SCI.

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Enzymes in Flow Injection Analysis

Koncki

Tymecki

Rozum

2008

Advances in Flow Analysis

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Development of an Amperometric Biosensor Platform for the Combined Determination of l-Malic, Fumaric, and l-Aspartic Acid

Röhlen

Pilas

Schöning

et al. 2017

Appl Biochem Biotechnol

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Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM (L-malate biosensor) and 0.4 μA mM (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0-10.0 mM with a sensitivity of 0.09 μA mM. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.

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Flow injection system for on-line monitoring of fumaric acid in biological processes

Cited by 9 publications

References 20 publications

Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

Sampling, Dilution, and Loading Device-coupled HighPerformance Liquid Chromatography Method for Successive On-line Analyses of Major Carbohydrate Products in Immobilized Cellulase Hydrolysate of Paper Cellulose

Enzymes in Flow Injection Analysis

Development of an Amperometric Biosensor Platform for the Combined Determination of l-Malic, Fumaric, and l-Aspartic Acid

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