Flow injection system for hydrolysable tannin determination

Bossu, Carla Maíra; Ferreira, E.; Chaves, F. S.; Menezes, E. A.; Nogueira, Ana Rita A.

doi:10.1016/j.microc.2006.04.022

Cited by 24 publications

(14 citation statements)

References 16 publications

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“…The hydrolysable tannin concentration was determined using potassium iodide according to [ 28 ]. One milliliter of saturated potassium iodide solution was added to the methanol solution containing 3 mL of pulp, peel, and endocarp (10 mg/mL) and allowed to rest at room temperature for 40 min and centrifuged for 4 minutes at 4000 rpm and the absorbance was measured at 550 nm.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and Ex Vivo Chemopreventive Action of Mauritia flexuosa Products

Pereira-Freire

Oliveira

Lima

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Mauritia flexuosa (Arecaceae), known as “Buriti,” is a Brazilian palm tree with high economic potential for local communities. Herein, we investigated the phytochemistry profile and antioxidant potential of M. flexuosa fruits and determined the bioaccessibility of phenolic compounds. Peels revealed upper values for phenols, flavonoids, carotenoids, tannins, and ascorbic acid when compared to the pulps and endocarps. All samples showed capacity to scavenger free radicals (0.5, 1.0, 2.0, 4.0, and 8.0 mg/mL) but peels presented higher scavenger action in all methods explored. Phenolic compounds identified by HPLC displayed reduced bioaccessibility after in vitro simulated gastrointestinal digestion for pulp (38.7%), peel (18.7%), and endocarp (22.3%) extracts (P < 0.05). Buriti fruits also protected rat blood cells against lysis induced by peroxyl radicals. We demonstrated the promising chemopreventive potentialities of M. flexuosa fruits and their by-products and peels with higher quantities of bioactive compounds and phenolic substances before and after in vitro bioaccessibility investigation. In Brazil, these parts are discarded or underused, mainly as feed for ruminant animals. Consequently, it is extremely important to explore nutritional characteristics of these by-products for human/livestock foods and to install biofriendly techniques and sustainable biotechnology handling of natural resources.

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Section: Methodsmentioning

confidence: 99%

In Vitro and Ex Vivo Chemopreventive Action of Mauritia flexuosa Products

Pereira-Freire

Oliveira

Lima

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Hydrolysable tannins content was determined with potassium iodate test described by Bossu et al (2006) method. Five milliliter of aqueous solution KIO 3 (2.5% v/v) was heated for 7 min at 30°C, then 1 mL of the aqueous solution (diluted 5 times), was added.…”

Section: Determination Of Hydrolysable Tannins Contentsmentioning

confidence: 99%

Green extraction process of tannins obtained from Moroccan Acacia mollissima barks by microwave: Modeling and optimization of the process using the response surface methodology RSM

Rhazi

Hannache

Oumam

et al. 2019

Arabian Journal of Chemistry

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The effect of extraction conditions on polyphenols contents and condensed tannins by microwave-assisted extraction (MAE) was studied for the first time to our knowledge. Moroccan barks of Acacia mollissima was used to extract phenolic compounds. The variables studied are the following: power extraction, time extraction and solvent nature. Five powers extraction were tested: 150 W, 250 W, 300 W, 450 W and 600 W. A significant effect of power extraction on the extractable nature was proved by ANOVA and Student test. The yields were also affected by time extraction. Different solvent (water, ethanol, methanol and ethyl acetate) were tested to evaluate the best extraction solvent according to the extractable nature. Highest polyphenols contents were obtained with methanol. The proportion of this solvent, time extraction and power extraction were optimized using the response surface methodology (RSM). A face-centered composite design (FCCD) was applied to evaluate the effects of these variables on the polyphenols and condensed tannins contents. For each experiment, the extraction yield, the total polyphenolic contents and

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“…Evaluation of the stability of this transient product revealed that the color reached maximum absorbance after 20 min of reaction, with this value being maintained for 15 min, after which the color started to disappear, with formation of a yellow product. The formation of this yellow degradation product has been described previously (Bossu et al 2006).…”

Section: Resultsmentioning

confidence: 81%

A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples

et al. 2016

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This paper describes the development of a rapid and clean method for the determination of hydrolysable tannins in honey samples, focusing on an easy and green sample pretreatment and low generation of waste. The proposed method is based on the reaction between KIO 3 and hydrolysable tannin compounds in acetate buffer medium (pH 4.75), with formation of a colored product measured at 525 nm. The analytical conditions were optimized using experimental design (central composite design). The linear range obtained was 40.0-740 mg L −1 (r = 0.998), and the detection (LOD) and quantification (LOQ) limits were 12.2 and 40.8 mg L −1 , respectively. Acetate buffer was used to ensure formation of the colored product obtained by reaction between the chromogenic reagent and the analyte. The method showed good results when applied to honey samples, with recoveries in the range of 78.3-96.1 %, and the use of diffuse reflectance spectroscopy coupled with spot test analysis provided low reagent consumption and minimized waste generation. The results obtained with the proposed method were confirmed by a spectrophotometric method.

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Flow injection system for hydrolysable tannin determination

Cited by 24 publications

References 16 publications

In Vitro and Ex Vivo Chemopreventive Action of Mauritia flexuosa Products

In Vitro and Ex Vivo Chemopreventive Action of Mauritia flexuosa Products

Green extraction process of tannins obtained from Moroccan Acacia mollissima barks by microwave: Modeling and optimization of the process using the response surface methodology RSM

A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples

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