Flow cytometric immunoassay for sulfonamides in raw milk

Keizer, W. de; Bienenmann-Ploum, M.E.; Bergwerff, Aldert A.; Haasnoot, W.

doi:10.1016/j.aca.2008.05.013

Cited by 53 publications

(19 citation statements)

References 15 publications

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“…In this field it has been employed to detect: soy, pea and soluble wheat protein in milk powder [16], veterinary antibiotic sulphonamides in milk [17], and chemical contaminants such as polycyclic aromatic hydrocarbons which are carcinogenic materials formed from incomplete combustion of organic materials during industrial processing [18] and mycotoxins in feed [19] or in wheat and cereal [20].…”

Section: Introductionmentioning

confidence: 99%

Detection of Allergens in a Multiple Allergen Matrix and Study of the Impact of Thermal Processing

Gomaa¹,

Ribéreau²,

Boye³

2016

J Nutr Food Sci

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Sciences Gomaa et al., J Nutr Food Sci 2012, S9 http://dx.doi.org/10.4172/2155 AbstractThe objectives of this work were to (a) determine the percentage recovery of three allergens (casein, egg and soy) simultaneously incurred in a flour matrix, (b) investigate the effect of different baking periods on allergen recovery using two different methods of allergen detection, namely enzyme linked immunosorbent assay (ELISA) and flow cytometry, and c) determine the solubility of proteins in the prepared blank dry cookie mix flour, non-cooked dough and baked cookies after baking at different temperatures using different extraction buffers. Sodium carbonate buffer was least effective in extracting protein. For the blank flour and non-cooked samples, PBS buffer was the most effective at extracting protein, whereas the Tris buffer gave the highest protein recoveries among the three buffers for the cooked samples. For all three allergens, thermal processing greatly reduced allergen recovery in the processed food matrix as detected using both the commercial ELISA kits and flow cytometry. Moreover, allergen recovery in the cooked samples decreased with increasing processing temperatures and ranged from 96% to 20% for casein, 26.5% to 3.7% for soy and 12.8% to 0% for egg in an instance where a false negative was detected when a high processing temperature of 450°F was employed.

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Section: Introductionmentioning

confidence: 99%

Detection of Allergens in a Multiple Allergen Matrix and Study of the Impact of Thermal Processing

Gomaa¹,

Ribéreau²,

Boye³

2016

J Nutr Food Sci

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“…Special attention has been paid to the relationship between the cytotoxic effect of sulfonamides and their enzyme (carbonic anhydrases, cyclooxygenase-2 and dihydrofolate reductase) inhibitory activity [7][8][9]. Sulfonamides are of considerable interest and have been used as pharmaceutical agents for the treatment of different diseases such as infections [10], Alzheimer's disease [11] and HIV [12]. Sulfa drugs are still widely used for conditions such as acne and urinary tract infections, and are receiving renewed interest for the treatment of infections caused by bacteria resistant to other antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Alkyl sulfonic acide hydrazides: Synthesis, characterization, computational studies and anticancer, antibacterial, anticarbonic anhydrase II (hCA II) activities

Özdemir

İlbiz

Gündüzalp

et al. 2015

Journal of Molecular Structure

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“…However, such immunoassays are considered as screening assays due to the risk of false non-compliant results and subsequent confirmation with instruments such as liquid chromatography (LC) combined with mass spectrometry (MS) is compulsory [8]. Screening assays with multi-analyte reagents (group-specific antibodies [9], transport proteins [10], or receptors [11]) are of particular interest since they might pinpoint the occurrence of emerging yet unidentified food contaminants and the subsequent MS identification of the interacting compound(s) is essential. In an ideal situation, to avoid different sample preparations with different selectivities, the screening should be as close as possible to the MS confirmation or identification of unknowns, which could be achieved by using identical bioreagents in both methods.…”

Section: Introductionmentioning

confidence: 99%

“…Other distinct advantages are high-throughput capability, versatility, accuracy and reproducibility [14]. Multiplex FCIAs were described for the screening of plant proteins, which might be used as adulterants in milk powders [15], pathogens [16], mycotoxins [17], sulfonamides in milk [9] and polycyclic aromatic hydrocarbons in fish [18]. However, all of these are indirect inhibition FCIAs and most of them use non-magnetic beads (MicroPlex®) coated with antigens and fluorescent labelled (secondary) antibodies for detection.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic microbeads for screening with flow cytometry and identification with nano-liquid chromatography mass spectrometry of ochratoxins in wheat and cereal

et al. 2011

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Multi-analyte binding assays for rapid screening of food contaminants require mass spectrometric identification of compound(s) in suspect samples. An optimal combination is obtained when the same bioreagents are used in both methods; moreover, miniaturisation is important because of the high costs of bioreagents. A concept is demonstrated using superparamagnetic microbeads coated with monoclonal antibodies (Mabs) in a novel direct inhibition flow cytometric immunoassay (FCIA) plus immunoaffinity isolation prior to identification by nano-liquid chromatography–quadrupole time-of-flight-mass spectrometry (nano-LC-Q-ToF-MS). As a model system, the mycotoxin ochratoxin A (OTA) and cross-reacting mycotoxin analogues were analysed in wheat and cereal samples, after a simple extraction, using the FCIA with anti-OTA Mabs. The limit of detection for OTA was 0.15 ng/g, which is far below the lowest maximum level of 3 ng/g established by the European Union. In the immunomagnetic isolation method, a 350-times-higher amount of beads was used to trap ochratoxins from sample extracts. Following a wash step, bound ochratoxins were dissociated from the Mabs using a small volume of acidified acetonitrile/water (2/8 v/v) prior to separation plus identification with nano-LC-Q-ToF-MS. In screened suspect naturally contaminated samples, OTA and its non-chlorinated analogue ochratoxin B were successfully identified by full scan accurate mass spectrometry as a proof of concept for identification of unknown but cross-reacting emerging mycotoxins. Due to the miniaturisation and bioaffinity isolation, this concept might be applicable for the use of other and more expensive bioreagents such as transport proteins and receptors for screening and identification of known and unknown (or masked) emerging food contaminants.FigureMicrobead coated with antibody

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Flow cytometric immunoassay for sulfonamides in raw milk

Cited by 53 publications

References 15 publications

Detection of Allergens in a Multiple Allergen Matrix and Study of the Impact of Thermal Processing

Detection of Allergens in a Multiple Allergen Matrix and Study of the Impact of Thermal Processing

Alkyl sulfonic acide hydrazides: Synthesis, characterization, computational studies and anticancer, antibacterial, anticarbonic anhydrase II (hCA II) activities

Immunomagnetic microbeads for screening with flow cytometry and identification with nano-liquid chromatography mass spectrometry of ochratoxins in wheat and cereal

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