Flow cytometer-on-a-chip

The next generation of hybrid microfluidic/integrated circuit chips: recent and upcoming advances in high-speed, high-throughput, and multifunctional lab-on-IC systems

“…3D) with two channels that successively measure passing cells at 0.9 GHz and 2.6 GHz. 75,76 Instead of using an oscillator for readout (which measures the shift in the resonator's stored energy and therefore is only sensitive to the particle's susceptibility magnitude 77 ), the chip measures cells by observing their effect on the coupling between two on-chip concentric coils, realizing an integrated transformer. Both the magnitude and phase of the MNP label susceptibility can be extracted using a quadrature receiver to downconvert the GHz signals to low frequencies for detection (Fig.…”

Quantitative Magnetic Flow Cytometry in High Hematocrit Conditions for Point-of-Care Testing

“…Challenges such as extensive sample pretreatment, expensive and bulky optics with limited portability, and complex workflow integration hinder compliance with the REASSURED criteria. To overcome this restraining complexity associated with optical readout systems, magnetic flow cytometers (MFC) with non-optical cell characterization have emerged as an alternative to OFC 14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30 . Utilizing immunologically functionalized magnetic nanoparticles (MNP) instead of fluorescent labels, MFCs employ magnetoresistive sensing mechanisms embedded within microfluidic systems, facilitating miniaturization, cost-effective production, and highly integrated workflows 28 .…”