The next generation of hybrid microfluidic/integrated circuit chips: recent and upcoming advances in high-speed, high-throughput, and multifunctional lab-on-IC systems

Iyer, Vasant; Issadore, David; Aflatouni, Firooz

doi:10.1039/d2lc01163h

Cited by 6 publications

(6 citation statements)

References 209 publications

(257 reference statements)

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“…Our MEA technique provides one of the smallest electrode separations (∼6 µm) and therefore highest spatial resolution reported in literature [reviewed in ( Iyer et al, 2023 ), but see ( Laborde et al, 2015 ; Widdershoven et al, 2018 )] enabling the non-invasive detection of non-electrogenic single cells, but also cell network studies. CAN spectroscopy electrically images only viable cells that are attached to the CMOS MEA’s oxide surface, while brightfield microscopy images all cells present on the chip adhered or not.…”

Section: Discussionmentioning

confidence: 99%

Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

Ell,

Bui,

Kigili

et al. 2024

Front. Bioeng. Biotechnol.

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With cancer as one of the leading causes of death worldwide, there is a need for the development of accurate, cost-effective, easy-to-use, and fast drug-testing assays. While the NCI 60 cell-line screening as the gold standard is based on a colorimetric assay, monitoring cells electrically constitutes a label-free and non-invasive tool to assess the cytotoxic effects of a chemotherapeutic treatment on cancer cells. For decades, impedance-based cellular assays extensively investigated various cell characteristics affected by drug treatment but lack spatiotemporal resolution. With progress in microelectrode fabrication, high-density Complementary Metal Oxide Semiconductor (CMOS)-based microelectrode arrays (MEAs) with subcellular resolution and time-continuous recording capability emerged as a potent alternative. In this article, we present a new cell adhesion noise (CAN)-based electrical imaging technique to expand CMOS MEA cell-biology applications: CAN spectroscopy enables drug screening quantification with single-cell spatial resolution. The chemotherapeutic agent 5-Fluorouracil exerts a cytotoxic effect on colorectal cancer (CRC) cells hampering cell proliferation and lowering cell viability. For proof-of-concept, we found sufficient accuracy and reproducibility for CAN spectroscopy compared to a commercially available standard colorimetric biological assay. This label-free, non-invasive, and fast electrical imaging technique complements standardized cancer screening methods with significant advances over established impedance-based approaches.

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Section: Discussionmentioning

confidence: 99%

Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

Ell,

Bui,

Kigili

et al. 2024

Front. Bioeng. Biotechnol.

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“…Research about the LOCs themselves is also being conducted to expand their capabilities; ongoing efforts in this field are steadily moving forward toward incorporating integrated circuits to obtain LOC-IC hybrid devices capable of high-performance applications, such as high-throughput integrated flow cytometers and high-speed biosensors with high temporal resolution. , Exposing chemistry undergraduates to LOC technologies early in their undergraduate studies can not only impart the skills required in the field but also cultivate curiosity and innovation, which will be vital in an emerging and evolving domain like LOCs.…”

Section: Lab-on-a-chip (Loc) Technologiesmentioning

confidence: 99%

Keeping Analytical Chemistry Training Up-to-Date

Fung,

Widyantoro,

2024

Anal. Chem.

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The undergraduate analytical chemistry curriculum serves to equip students with the knowledge and skills for work outside of classroom training. As such, instructors face a challenging task in deciding the breadth and depth of topics for their courses to ensure their syllabi can remain up-to-date with today's needs. We propose that instructors consider covering capillary electrophoresis (CE) and lab-on-a-chip (LOC) technologies in their analytical chemistry courses. Past surveys of the curriculum show a noticeable lack of emphasis on these topics, which we feel is a missed opportunity and one that holds potential for the collective benefit of instructors and students. CE and LOCs are utilized in a diverse array of fields like biochemistry, pharmaceutical production, materials science, and environmental analysis, and their applications are becoming increasingly important amidst the growing movement toward environmentally sustainable practices and green chemistry. They are also more accessible in the analytical chemistry classroom compared with typical benchtop instruments due to the flexibility of their size and cost. This makes them easier to obtain, maintain, and transport for use and demonstration purposes. Additionally, interwoven in these topics are core concepts that are fundamental to analytical chemistry; thus, covering them will inherently reinforce students' understanding of fundamental knowledge. Therefore, we believe increased coverage of CE and LOCs can better prepare undergraduates for modern analytical chemistry work in various industries and fields of research.

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“…7 Biochemically functionalized field-effect transistor (FET) devices are well-suited for this application, since that target biomarkers in blood and interstitial fluid samples can directly provide electrical excitations via ionization in aqueous media. 8,9 Furthermore, the integration potential of FET devices in microfluidic systems enables convenient marker tests on-chip, thereby streamlining the analysis process. 10,11 Despite the potential benefits of the envisioned microfluidic biochip scenarios, their feasibility remains hindered due to the pathogenesis of cancers.…”

Section: Introductionmentioning

confidence: 99%

Multiplexed immunosensing of cancer biomarkers on a split-float-gate graphene transistor microfluidic biochip

Wang,

Gao

et al. 2024

Lab Chip

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The next generation of hybrid microfluidic/integrated circuit chips: recent and upcoming advances in high-speed, high-throughput, and multifunctional lab-on-IC systems

Abstract: Since the field’s inception, pioneers in microfluidics have made significant progress towards realizing complete lab-on-chip systems capable of sophisticated sample analysis and processing. One avenue towards this goal has been...

Cited by 6 publications

References 209 publications

Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

Assessment of chemotherapeutic effects on cancer cells using adhesion noise spectroscopy

Keeping Analytical Chemistry Training Up-to-Date

Multiplexed immunosensing of cancer biomarkers on a split-float-gate graphene transistor microfluidic biochip

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