Flexible fate determination ensures robust differentiation in the hair follicle

Xin, Tianchi; Gonzalez, David G.; Rompolas, Panteleimon; Greco, Valentina

doi:10.1038/s41556-018-0232-y

Cited by 40 publications

(59 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lateral disc cells were originally hypothesized to generate the new secondary hair germ during catagen (Panteleyev et al, 2001), however recent studies demonstrated through lineage tracing that Shh--expressing cells instead generate IRS lineages and their traced progeny does not survive catagen (i.e. is not the origin of the secondary hair germ) (Mesler et al, 2017;Panteleyev, 2018;Xin et al, 2018). The transcriptomes of the OID--forming cells did not however cluster together with the IRS populations in our data.…”

Section: Six Different Cell Populations With Outer--layer Signatures contrasting

confidence: 65%

The molecular anatomy of mouse skin during hair growth and rest

Joost

Annusver

Jacob

et al. 2019

Preprint

View full text Add to dashboard Cite

HIGHLIGHTS--Comprehensive single--cell transcriptome atlas of full--thickness skin --Outer root sheath (ORS) is composed of two distinct cell types --Companion layer transcriptionally resembles ORS --Transcriptional reconstruction of the internal hair follicle (HF) lineages --Molecular identification of an asymmetric HF--bulb structure --Spatial map of fibroblast subtypes in the skin --Online tool. http://kasperlab.org/tools 2 SUMMARYSkin homeostasis is orchestrated by dozens of cell types that together direct stem cell renewal, lineage commitment and differentiation. However, a systematic molecular atlas of full--thickness skin is lacking. Here, we used single--cell RNA--sequencing and mRNA--FISH to determine gene-expression identity and spatial location of skin cells during hair growth and rest. We defined 55 cell populations and made striking discoveries about the outer root sheath (ORS) and inner hair follicle layers that together coordinate hair production. The ORS is composed of two distinct cell types, companion layer cells resemble ORS and not inner layer cells, and we identified an asymmetric inner--layer structure with ORS cell identity. Moreover, the inner layers branch from transcriptionally uncommitted progenitors, and each lineage differentiation passes through an intermediate state. Altogether, we generated a comprehensive atlas with molecular and spatial information on epithelial and stromal cells, including fibroblasts, vascular and immune cells, that will spur new discoveries in skin biology.3

show abstract

Section: Six Different Cell Populations With Outer--layer Signatures contrasting

confidence: 65%

The molecular anatomy of mouse skin during hair growth and rest

Joost

Annusver

Jacob

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…[29,30] The presence of SCs or cells with SC-like properties in the lower segment of anagen HFs is supported in part by the fact that cells from this part exhibit colony forming ability, a property of SCs. [42] Recent work in AHFR and physiological anagen unveils the high plasticity of extra-bulge epithelial cells in the outer root sheath and the lower proximal cup, [18,23,53] supporting Chase's hypothesis.…”

Section: Con Clus Ionmentioning

confidence: 91%

“…Since the experiments involved surgical damage and cell transplantation, it is unclear whether HF epithelial cells do exhibit such a “flowing” nature in the normal physiological state. Recently, through lineage tracing and intravital imaging in mouse pelage HFs, it was shown that germinative cells are constantly lost and replenished by cells from the lower proximal cup . These two models, “static” vs “flowing,” are seemingly contradictory.…”

Section: Static Vs Flowing Dynamics In the Lower Segment Of Anagen Hamentioning

confidence: 99%

“…This will be the shortest route for the cells to travel to the zones of cell loss. Alternatively, these cells move along the basement membrane to the germinative zone in a process similar to the “flowing” model, but at a faster rate. Since these extra‐bulge basal cells abut dermal sheath across basement membrane, damaged dermal sheath cells might promote their movement through epithelial‐mesenchymal interaction.…”

Section: Questions To Be Solved In Ahfrmentioning

confidence: 99%

“…Recently, through lineage tracing and intravital imaging in mouse pelage HFs, it was shown that germinative cells are constantly lost and replenished by cells from the lower proximal cup. [53] These two models, "static" vs "flowing," are seemingly contradictory.…”

Section: S Tati C Vs Flowing Dynami C S In the Lower S Eg Ment Of Amentioning

confidence: 99%

See 2 more Smart Citations

Anagen hair follicle repair: Timely regenerative attempts from plastic extra‐bulge epithelial cells

Huang

Lin

Hsu

et al. 2019

Experimental Dermatology

View full text Add to dashboard Cite

Anagen hair follicle repair (AHFR) is the regenerative scheme activated to restore the structure and hair growth following injuries to anagen hair follicles. Compared with telogen‐to‐anagen regeneration and hair follicle neogenesis, AHFR is a clinically important, yet relatively unexplored regenerative feature of hair follicles. Due to their highly proliferative character, germinative cells and matrix cells within hair bulbs are highly susceptible to injuries, such as chemotherapy and radiotherapy. Clinical and experimental observations suggest that damaged anagen hair follicles are able to repair themselves to resume anagen growth, bypassing premature catagen/telogen entry. Mechanistically, extra‐bulge epithelial cells in the outer root sheath and the lower proximal cup are quickly mobilized for regeneration. These cells acquire stem cell‐like properties, exhibiting high plasticity by breaking lineage restriction to regenerate all cell types in the lower segment of anagen hair follicles. Facilitating extra‐bulge epithelial cells’ mobilization ameliorates hair loss from chemo‐ and radiotherapy. On the other hand, quiescent bulge stem cells can also be activated, but only after more severe injuries and with slower activation dynamics. They show limited plasticity and regenerate part of the outer root sheath only. The dysrhythmic activation might render bulge stem cells susceptible to concomitant injuries due to their exit from quiescence.

show abstract

The Hair Follicle as an Interdisciplinary Model for Biomedical Research: An Eclectic Literature Synthesis

Haslam

Paus

2020

BioEssays

View full text Add to dashboard Cite

Skin is a comparatively accessible organ possessing many conserved regulatory and signaling pathways, drawing researchers from varied fields toward its study. Hair follicle (HF) biology in particular has expanded rapidly over the preceding decade, helping to shape and develop scientific knowledge across diverse areas of biomedical research, beyond the skin. The hope in compiling this review is to inspire more researchers to utilize the HF as an instructive biological model, bringing with them fresh perspectives and experience from differing fields of study. The authors also wish to further motivate seasoned hair researchers to explore the further reaches of their understanding and the discoveries yet to be made. For this reason, the authors have endeavored to collate an eclectic mix of some of the most thought-provoking and scientifically intriguing articles associated with the field of HF research, published in the preceding two years.

show abstract

Flexible fate determination ensures robust differentiation in the hair follicle

Cited by 40 publications

References 47 publications

The molecular anatomy of mouse skin during hair growth and rest

The molecular anatomy of mouse skin during hair growth and rest

Anagen hair follicle repair: Timely regenerative attempts from plastic extra‐bulge epithelial cells

The Hair Follicle as an Interdisciplinary Model for Biomedical Research: An Eclectic Literature Synthesis

Contact Info

Product

Resources

About