Flavonoids from Whole-Grain Oat Alleviated High-Fat Diet-Induced Hyperlipidemia <i>via</i> Regulating Bile Acid Metabolism and Gut Microbiota in Mice

Duan, Ruiqian; Guan, Xiao; Huang, Kai; Zhang, Yu; Li, Sen; Xia, Jian Chuan; Shen, Meng

doi:10.1021/acs.jafc.1c01813

Cited by 126 publications

(96 citation statements)

References 52 publications

(93 reference statements)

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“…In this study, negative correlation between Lachnospiraceae_ND3007_Group with BMI and fat deposition was identified for the first time. In previous reports, Colidextribacter and Alloprevotella were positively correlated with hyperlipidemia in rats and daily gain in pigs, respectively [ 49 , 50 ], and we found them in the cecum of high-BMI Hu sheep. In particular, Saccharivibrio belonging to Proteobacteria presented high relative abundance in the cecum of high-BMI Hu sheep.…”

Section: Discussionsupporting

confidence: 71%

Distribution and Difference of Gastrointestinal Flora in Sheep with Different Body Mass Index

Cheng

Wang

Zhang

et al. 2022

Animals

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Fat deposition is the key factor affecting the efficiency of animal husbandry production. There are many factors affecting fat deposition, in which the gastrointestinal microbiota plays an important role. Therefore, the body mass index (BMI) was introduced into the evaluation of sheep fat deposition, and the different microbiota and functional pathways of the sheep gastrointestinal tract in different BMI groups were analyzed. We selected 5% of individuals with the highest and lowest BMI from a feed test population (357 in whole group). Microorganisms in 10 sites of the gastrointestinal tract in 36 individuals (18 in each group) were evaluated by 16S rRNA V3–V4 region sequencing. There were differences (p < 0.05) in fat deposition traits between different BMI groups. In the 10 parts of the gastrointestinal tract, the diversity and richness of cecal microflora in the high-BMI group were higher than those in low-BMI Hu sheep (p < 0.05). Principal coordinate analysis (PCoA) showed that there was separation of the cecum between groups, and there were differences in the cecal microbial community. Linear discriminant analysis effect size (LEfSe) showed that most biomarkers were in the cecum. On the basis of an indepth study of cecal microorganisms, 26 different bacterial genera were obtained (p < 0.05). Correlation analysis between them and the characteristics of fat deposition in sheep showed that Colidextribacter, Alloprevotella, and Succenivibrio were positively correlated with fat deposition, while Lachnospiraceae_ND3007_Group was negatively correlated (p < 0.05). The above results show that the cecum may be an important part leading to the difference of BMI in sheep, and its microorganisms may affect the level of fat deposition.

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Section: Discussionsupporting

confidence: 71%

Distribution and Difference of Gastrointestinal Flora in Sheep with Different Body Mass Index

Cheng

Wang

Zhang

et al. 2022

Animals

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“…In addition, the mRNA expressions of key genes including cholesterol-7α-hydroxylase ( CYP7A1 ), bile salt export pump ( BSEP ) and Na+/taurocholate cotransporting polypeptide ( NTCP ) related to bile acid biosynthesis and metabolism were analyzed ( Duan et al, 2021 ). Compared with the Control group, the transcription levels of CYP7A1 , BSEP and NTCP were obviously down-regulated in mice with excessive alcohol intake (the Model group) ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake

Guo

Cao

You

et al. 2022

Current Research in Food Science

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“…Additionally, the fundamental effect of P. gingivalis on the gut microbiota was evident after the change in diet (TP3 in Figure 4B ). Although the role of these bacteria in liver pathology has not been clarified, Colidextribacter has recently been reported to be positively correlated with serum levels of oxidative markers ( 50 ) and hyperlipidemia-related parameters ( 51 ). In addition, functional enrichment analysis of the expression profiles of genes in the liver demonstrated that the genes involved in the response to oxidative stress were enriched (Cluster 4) in Pg mice.…”

Section: Discussionmentioning

confidence: 99%

Oral Pathobiont-Induced Changes in Gut Microbiota Aggravate the Pathology of Nonalcoholic Fatty Liver Disease in Mice

et al. 2021

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Background & AimsPeriodontitis increases the risk of nonalcoholic fatty liver disease (NAFLD); however, the underlying mechanisms are unclear. Here, we show that gut dysbiosis induced by oral administration of Porphyromonas gingivalis, a representative periodontopathic bacterium, is involved in the aggravation of NAFLD pathology.MethodsC57BL/6N mice were administered either vehicle, P. gingivalis, or Prevotella intermedia, another periodontopathic bacterium with weaker periodontal pathogenicity, followed by feeding on a choline-deficient, l-amino acid-defined, high-fat diet with 60 kcal% fat and 0.1% methionine (CDAHFD60). The gut microbial communities were analyzed by pyrosequencing the 16S ribosomal RNA genes. Metagenomic analysis was used to determine the relative abundance of the Kyoto Encyclopedia of Genes and Genomes pathways encoded in the gut microbiota. Serum metabolites were analyzed using nuclear magnetic resonance-based metabolomics coupled with multivariate statistical analyses. Hepatic gene expression profiles were analyzed via DNA microarray and quantitative polymerase chain reaction.ResultsCDAHFD60 feeding induced hepatic steatosis, and in combination with bacterial administration, it further aggravated NAFLD pathology, thereby increasing fibrosis. Gene expression analysis of liver samples revealed that genes involved in NAFLD pathology were perturbed, and the two bacteria induced distinct expression profiles. This might be due to quantitative and qualitative differences in the influx of bacterial products in the gut because the serum endotoxin levels, compositions of the gut microbiota, and serum metabolite profiles induced by the ingested P. intermedia and P. gingivalis were different.ConclusionsSwallowed periodontopathic bacteria aggravate NAFLD pathology, likely due to dysregulation of gene expression by inducing gut dysbiosis and subsequent influx of gut bacteria and/or bacterial products.

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Flavonoids from Whole-Grain Oat Alleviated High-Fat Diet-Induced Hyperlipidemia via Regulating Bile Acid Metabolism and Gut Microbiota in Mice

Cited by 126 publications

References 52 publications

Distribution and Difference of Gastrointestinal Flora in Sheep with Different Body Mass Index

Distribution and Difference of Gastrointestinal Flora in Sheep with Different Body Mass Index

Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake

Oral Pathobiont-Induced Changes in Gut Microbiota Aggravate the Pathology of Nonalcoholic Fatty Liver Disease in Mice

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