Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake

Guo, Weiling; Cao, Ying-Jia; You, Shi-Ze; Wu, Qi; Zhang, Fang; Han, Jun; Lv, Xu-Cong; Rao, Pingfan; Ai, Lianzhong; Ni, Li

doi:10.1016/j.crfs.2022.02.013

Cited by 35 publications

(30 citation statements)

References 78 publications

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“…Moreover, lipid peroxidation induces the production of peroxides and reactive oxygen species. The function of SOD is the depressor of reactive oxygen species and promoting the formation of hydrogen peroxide in vivo, which was further decomposed to the non-toxic substance (oxygen and water) by up-regulating GSH-Px activity [15]. Our study displayed that L. paracasei CCFM1222 pretreatment obviously suppressed the MDA concentration, and up-regulated the SOD and GSH-Px activities in LPS-treated mice.…”

Section: Discussionmentioning

confidence: 62%

“…The appropriate concentration of cecal SCFAs is help to keep the stable intestinal function, thus preventing the injury of liver function [30]. For example, acetic acid controls the host glucose metabolism by affecting the sensitivity of insulin [15]. Butyric acid not only supplied energy to intestinal epithelial cells, and promoted the mitosis of the immune cell, which is help to increase protection for the host [31].…”

Section: Discussionmentioning

confidence: 99%

“…L. plantarum ZY08 attenuates the long-time alcohol consumption-induced hepatic steatosis and liver damage by regulate the intestinal microbiota composition [14]. In addition, recently report also suggested that the "gut-liver" axis takes a vital role in the pathological process of some liver diseases [15]. Metabolites from intestinal ora are regarded as the vital medium in the "gut-liver" axis, such as short-chain fatty acids (SCFAs), amino acids, and bile acids.…”

Section: Introductionmentioning

confidence: 98%

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Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice

Guo

Cui

Tang

et al. 2022

Probiotics & Antimicro. Prot.

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In recent years, acute liver injury (ALI) has received wide-range attention in the world due to its relatively high morbidity and mortality. This study aimed to explore the hepatoprotective effect of Lactobacillus paracasei CCFM1222 against lipopolysaccharide (LPS)-induced ALI mice, and further elaborate its mechanism of action from the perspective of intestinal microbiomics and metabolomics. The results displayed that L. paracasei CCFM1222 pretreatment signi cantly decreased the serum ALT, and AST levels, inhibited the releases of hepatic TNF-α, IL-1β, and IL-6 levels, and activated the SOD, CAT, and GSH-Px activities in LPS-treated mice. The cecal short-chain fatty acid (SCFAs) levels were increased in LPStreated mice with L. paracasei CCFM1222 pretreatment. In addition, L. paracasei CCFM1222 pretreatment remarkably shifted the intestinal microbiota composition, including the higher abundance of Faecalibaculum, Bi dobacterium, and lower abundance of Prevotellaceae NK3B31 group, which is positively associated with the cecal propionic, butyric, valeric, isobutyric, and isovaleric acids. The metabolomics based on UPLC-QTOF/MS revealed that L. paracasei CCFM1222 pretreatment signi cantly regulated the composition of feces metabolites in LPS-treated mice, especially the potential biomarkersrelated butanoate metabolism, vitamin B6 metabolism, D-glutamine and D-glutamate metabolism, tryptophan metabolism, caffeine metabolism, arginine biosynthesis, arginine, and proline metabolism. Moreover, L. paracasei CCFM1222 pretreatment remarkably regulated the expression of genes-associated ALI (including Tlr4, Myd88, Nf-kβ, iNOS, Cox2, Iκ-Bα, Nrf2, and Sirt-1). In conclusion, these results suggest the possibility that L. paracasei CCFM1222 supplementation has bene cial effects on preventing the occurrence and development of ALI by inhibiting the in ammatory responses, altering intestinal microbiota composition and their metabolites, and mediating the Tlr4/Nf-kβ pathway.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice

Guo

Cui

Tang

et al. 2022

Probiotics & Antimicro. Prot.

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“…Tenacissoside G, Tenacissimoside H and Tenacissoside I possess promising potential in anti-inflammatory, cancer chemopreventive, and neuroprotective medicines dietary or supplements ( Liu et al, 2008 ; Wang et al, 2021 ). Ganoderic acids protect against alcoholic liver injury and modulate intestinal microbiota in mice with excessive alcohol intake ( Guo et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Nontargeted metabolomics study and pharmacodynamic evaluation of bidirectional fermentation for Ganoderma lucidum with Marsdenia tenacissima

Zhang²,

Su³

et al. 2022

Front. Pharmacol.

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Lung cancer is one of the malignant tumors with the fastest incidence rate and mortality growth and the greatest threat to human health and life. Marsdenia tenacissima is an antitumor of Chinese medicine. However, Marsdenia tenacissima has low bioavailability in the human body and most of its main active substances are aglycones, such as Tenacigenin A, Tenacigenin B. This study aims to produce biotransformation products rich in pungent saponins by using Marsdenia tenacissima as a fermentation medium of Ganoderma lucidum. Non-targeted metabolomics analysis was carried out on the fermentation products after the optimization process. A total of 249 differential metabolites were detected, and the content of saponins increased from 0.1% to 0.41% and most of them were tenacigenin. Furthermore, the biotransformation of C21 steroidal glycosides in Marsdenia tenacissima was the central reaction in this fermentation process. Pharmacodynamics resewed that the anticancer effect of Marsdenia tenacissima was significantly enhanced after fermentation, mainly through inhibiting the growth and apoptosis of cancer cells.

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“…Here, we observed that L. paracasei CCFM1223 pretreatment against LPS treatment induced the elevation of hepatic MDA level and the reduction of antioxidative enzyme activities (T-AOC, SOD, GSH-Px, and CAT). The action of SOD is the inhibition of reactive oxygen species (ROS) and the boost of hydrogen peroxide formation in vivo, which was further decomposed to non-toxic substances (oxygen and water) under the higher activity of GSH-Px [ 23 ]. LPS intraperitoneal injection accelerates the production of ROS from inflammatory cells, which further aggravates ALI and leads to chronic inflammation.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

et al. 2022

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Background: Lactobacillus paracasei CCFM1223, a probiotic previously isolated from the healthy people’s intestine, exerts the beneficial influence of preventing the development of inflammation. Methods: The aim of this research was to explore the beneficial effects of L. paracasei CCFM1223 to prevent lipopolysaccharide (LPS)-induced acute liver injury (ALI) and elaborate on its hepatoprotective mechanisms. Results: L. paracasei CCFM1223 pretreatment remarkably decreased the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in mice with LPS treatment and remarkably recovered LPS-induced the changes in inflammatory cytokines (tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), interleukin (IL)-1β, IL-6, IL-17, IL-10, and LPS) and antioxidative enzymes activities (total antioxidant capacity (T-AOC), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT)). Metagenomic analysis showed that L. paracasei CCFM1223 pretreatment remarkably increased the relative abundance of Catabacter compared with the LPS group but remarkably reduced the relative abundance of [Eubacterium] xylanophilumgroup, ASF356, LachnospiraceaeNK4A136group, and Lachnoclostridium, which is closely associated with the inflammation cytokines and antioxidative enzymes. Furthermore, L. paracasei CCFM1223 pretreatment remarkably increased the colonic, serum, and hepatic IL-22 levels in ALI mice. In addition, L. paracasei CCFM1223 pretreatment remarkably down-regulated the hepatic Tlr4 and Nf-kβ transcriptions and significantly up-regulated the hepatic Tlr9, Tak1, Iκ-Bα, and Nrf2 transcriptions in ALI mice. Conclusions: L. paracasei CCFM1223 has a hepatoprotective function in ameliorating LPS-induced ALI by regulating the “gut–liver” axis.

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Ganoderic acids-rich ethanol extract from Ganoderma lucidum protects against alcoholic liver injury and modulates intestinal microbiota in mice with excessive alcohol intake

Cited by 35 publications

References 78 publications

Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice

Intestinal Microbiomics and Metabolomics Insights into the Hepatoprotective Effects of Lactobacillus paracasei CCFM1222 Against the Acute Liver Injury in Mice

Nontargeted metabolomics study and pharmacodynamic evaluation of bidirectional fermentation for Ganoderma lucidum with Marsdenia tenacissima

Lactobacillus paracasei CCFM1223 Protects against Lipopolysaccharide-Induced Acute Liver Injury in Mice by Regulating the “Gut–Liver” Axis

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