Flavonoids from Dalbergia louvelii and Their Antiplasmodial Activity

Beldjoudi, Naïma; Mambu, Lengo; Labaïed, Mehdi; Grellier, Philippe; Ramanitrahasimbola, David; Rasoanaivo, Philippe; Martin, Marie Thérèse; Frappier, François

doi:10.1021/np030008x

Cited by 58 publications

(38 citation statements)

References 15 publications

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“…The monomer of compound 1 is liquiritigenin which has no antiplasmodial activity, while a chalcone (could be related to 1/2), namely isoliquiritigenin, has weaker in vitro antimalarial activity than the present biflavanone (1) [12]. Finally, biflavanone 1 could be a promising compound for investigation of in vivo antimalarial activity in animal models.…”

Section: Resultsmentioning

confidence: 99%

Antimalarial Activity of Biflavonoids from Ochna integerrima

Ichino¹,

Kiyohara²,

Soonthornchareonnon³

et al. 2006

Planta med

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IntroductionMalaria is the major parasitic infection in many tropical and subtropical regions, leading to more than one million deaths (principally among African children) out of 400 million cases each year [1] and to major consequent impacts on economic productivity and livelihood [2]. The incidence of malaria is now increasing because of the appearance of multi-drug resistant Plasmodium falcipalum, therefore new and more effective antimalarial drugs are urgently required.Ochna integerrima Merr. (Ochnaceae) is a tree that is widely distributed in Thailand [3], and the bark of O. integerrima Merr. has been used for digestive disorders as a folk medicine in Thailand [4]. In phytochemical studies on O. integerrima Merr, many flavonoids have been isolated [5], [6], [7]. During a screening for antimalarial active sources among Thai plants, it was found that the 80 % EtOH extract from the outer bark of O. integerrima Merr.showed significant anti-malarial activity. The active ingredients in the outer bark were clarified in the present study. Materials and Methods ApparatusOptical rotations were measured on a JASCO polarimeter. 1 H-and 13 C-NMR spectra were determined on a Varian Mercury-300. Mass spectra (MS) were obtained on JEOL MXA-AM505HA and JMS-700 MStation spectrometers. Chromatographic separations were carried out by column chromatography on Wakogel C-200 (75 ± 150 mm, Wako; Osaka, Japan). Preparative reverse phase HPLC was carried out on PEGASIL (250 20 mm i. d., Senshu Co. Ltd.; Tokyo, Japan). Antimalarial Activity of Biflavonoids from Ochna integerrimaChikara AbstractDuring the screening of antimalarial substances, the 80 % EtOH extract from the outer bark of Ochna integerrima Merr. (Ochnaceae) was shown to have a good anti-malarial activity (IC 50 value: 6.5 mg/mL) whereas extracts from the inner barks of O. integerrima showed no antimalarial activity. Biflavanone (1), which had not been found previously from a natural plant source, was isolated as a potent antimalarial active ingredient (IC 50 value: 80 ng/mL) from the extract of the outer barks. The stereoisomer of 1 ( = compound 2) was also isolated from this plant; however, its activity was significantly lower than that of 1. Key wordsAntimalarial´Ochnaceae´Ochna integerrima Merr.´biflavanones Original Paper 611This document was downloaded for personal use only. Unauthorized distribution is strictly prohibited. Analytical methodsAnalytical HPLC experiments were performed on an Agilent 1100 series HPLC instrument (Agilent Technologies Japan, Ltd.; Tokyo, Japan) equipped with a column of PEGASIL (250 4.6 mm i. d., Senshu Co. Ltd.; Tokyo, Japan). The solvent system used was a linear gradient of acetonitrile from 30 % to 60 % during 30 minutes in 10 mM phosphoric acid. Flow rate was 1 mL/min. Injection volume was 10 mL for MeOH solution of all the samples. Concentrations of the samples were 10 mg/mL for extracts and 1 mg/ mL for the isolated compounds. Plant material Extraction and isolationThe outer barks of O. integerrima (485 g) were extracted with 80...

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Section: Resultsmentioning

confidence: 99%

Antimalarial Activity of Biflavonoids from Ochna integerrima

Ichino¹,

Kiyohara²,

Soonthornchareonnon³

et al. 2006

Planta med

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“…In Brazil, most people in rural areas rely on traditional medicine for the treatment of many infectious diseases, and in some malaria endemic regions treatment is only provided by traditional herbal remedies. Natural molecules are structurally diverse, which makes them a valuable source of novel compounds (Beldjoudi et al, 2003;Napolitano et al, 2005), and may provide innovative strategies towards malarial control, such as the antimalarial drug artemisinin, initially isolated from Artemisia annua (Klayman, 1985;Li and Rieckmann, 1992). The present study investigates the in vitro antiplasmodial activity of hexanic and ethanolic extracts of plants used by the Brazilian Cerrado population to treat malaria, and other health problems, as well as their toxicity against two mammalian cell lines.…”

Section: Introductionmentioning

confidence: 99%

In vitro antiplasmodial activity of Brazilian Cerrado plants used as traditional remedies

Mesquita¹,

Grellier²,

Mambu³

et al. 2007

Journal of Ethnopharmacology

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“…1. These flavonoids showed various in vitro benefits to human health, such as anti-inflammatory, anticoagulant, antitumor, antihyperlipidic, antinephritic, antioxidant and vasodilative effects [22][23][24][25][26][27][28]. However, to what extent in vitro effects produced by these flavonoids could be extrapolated to the in vivo situation was poorly understood.…”

Section: Introductionmentioning

confidence: 99%