Flavonoids from Anoectochilus annamensis and their Anti-inflammatory Activity

Abstract

One new flavonol diglycoside, 4',5-dihydroxy-3,3',7-trimethoxyflavone 4'-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside (1), and two known compounds (2-3) were isolated from the methanolic extract of Anoectochilus annamensis Aver. aerial parts. The effects were evaluated of all isolated compounds (1-3) on LPS-induced production of the inflammatory mediator nitric oxide (NO) by RAW264.7 cells. 4',5-Dihydroxy-3,3',7-trimethoxyflavone (2) was the most active while the addition of a rutinoside at C-4' (compound 1) decreased the inhibitory activity. This is the first report on the chemical composition and biological activity of A. annamensis.

Quassinoids and Alkaloids From the Roots of Eurycoma longifolia

“…The effects of compounds on the NO production in LPS-stimulated RAW264.7 cells were evaluated as previously described. 10 Cardamonin was used as a positive control (IC 50 = 2.80 µM). The MTT assay showed that all compounds had no significant toxicity at their effective doses for the NO inhibition (data not shown).…”

A New Noriridoid and Six Phenolic Compounds from Rhopalocnemis phalloides

“…The effects of compounds on the NO production in LPS-stimulated RAW264.7 cells were evaluated as previously described [9]. Cardamonin was used as a positive control.…”