Evaluation of in vitro cytotoxicity and in vivo potential toxicity of the extract from in vitro cultivated Anoectochilus roxburghii Lindl

Chung, Đoàn Chính; Long, Lê Thanh; Ho, Nguyen Quynh Chi; Nguyen, Thi Thuy Nga; Giap, Dang; Thang, Duc; Hoang, Nghia Son

doi:10.1080/15287394.2021.1963363

Cited by 7 publications

(8 citation statements)

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“…BAX gene expression was signi cantly increased in A.roxburgii treated JURKAT and MM1S cells whereas BAX and BIM gene expression was signi cantly increased in A.roxburgii treated U266 cells compared with controls. The only study in the literature analyzing gene expression using this plant extract was Chung et al In this study, BAX expression was increased in MCF7 cells treated with cultured A. roxburghii in vitro (iARE), which is consistent with our results [2].…”

Section: Discussionsupporting

confidence: 91%

“…Another study has shown that cultured A. roxburghii can destroy cancer cells in vitro, including breast cancer MCF7 cells. In addition, the activation of the caspase-dependent intrinsic apoptotic pathway contributed to the majority of A. roxburghii-induced cytotoxicity in MCF7 cells [2].…”

Section: Discussionmentioning

confidence: 99%

“…[1]. It is estimated that 80% of people worldwide still use herbal therapies to treat various illnesses, including cancer, according to the World Health Organization (WHO) [2]. The widely recognized medicinal orchid Anoectochilus species, which is grown in Asian countries, contains a wide variety of substances, including avonoids, polysaccharides, glycosides and alkaloids, all of which have effective pharmacological uses [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Few studies have analyzed the in vitro biological activities of Anoectochilus species [2]. Owing to its antioxidant, antihyperglycemic, hepatoprotective, and vascular protective properties, Anoectochilus roxburghii and Anoectochilus formosanus are two species that are frequently used in traditional medicine in Vietnam, Taiwan, and China [2]. In previous studies, Shyur et al (2004), Ho et al (2018) demonstrated the anti-proliferative activity of A. formosanus or A. roxburghii, which is largely associated with the induction of apoptosis [6,7].…”

Section: Introductionmentioning

confidence: 99%

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The evaluation of the anti-cancer effects of Anoectochilus roxburghii on hematological cancers in vitro

Gunes,

Ozkan,

GONULKIRMAZ

et al. 2023

Preprint

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The cause of hematological cancers is the uncontrolled proliferation of hematopoietic and lymphoid tissues, and chemotherapy is used to treat cancer. However, adverse side effects of chemotherapy are common. Therefore, the use of plant extracts as a method for treating cancer is becoming increasingly popular. Anoectochilus roxburghii (wall.) Lindl. (A. roxburghii) is one of the original sources of the valuable medicinal plants known as the king medicine and the golden grass. This study investigated the potential anticancer effect of A. roxburghi (AR) on JURKAT, MM1S, THP1 and U266 cells. To test the cytotoxic and apoptotic effects of AR, hematological cancer cells were exposed to increasing doses of AR (0.1–0.5 µg/µl). The spectrophotometric MTT assay and the flow cytometric Annexin V staining were used to examine the viability and apoptosis of the cells, respectively. qRT-PCR was used to determine the expression levels of the apoptosis-related genes BAD, BAX, BIM and BCL-2. Our results show that AR treatment decreased cell viability and induced apoptosis in each cell line. Our RT-PCR data showed that AR significantly increased the expression levels of the pro-apoptotic BAX gene in JURKAT and MM1S cells, whereas it significantly increased the expression levels of both BAX and BIM in U266 cells. This is the first study to investigate how AR modulates apoptosis in hematological cancer cells. As a result, AR therapy may be a promising treatment modality for the treatment of cancer.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%