Flavonoids and Isoflavonoids from Sophorae Flos Improve Glucose Uptake<i>in Vitro</i>

Chen, Quan Cheng; Zhang, Wei Yun; Jin, Wenyi; Lee, Ik Soo; Min, Byung‐Sun; Jung, Hyun‐Ju; Na, MinKyun; Lee, Sang-Myung; Bae, KiHwan

doi:10.1055/s-0029-1185944

Cited by 44 publications

(32 citation statements)

References 22 publications

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“…The same effects were also observed for the HepG2-cells. Kaempferol has been previously reported to increase glucose uptake in porcine myotubes, 3T3-L1 adipocytes and HepG2-cells [13,122,123]. Quercetin has been found to increase the glucose uptake in 3T3-L1 adipocytes, but was ineffective in the HepG2-cell lines [123].…”

Section: Glucose-and Oleic Acid Uptake In Human Skeletal Muscle Cellsmentioning

confidence: 99%

Structure–activity relationship of immunomodulating pectins from elderberries

Ahmed

Zou

et al. 2015

Carbohydrate Polymers

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Section: Glucose-and Oleic Acid Uptake In Human Skeletal Muscle Cellsmentioning

confidence: 99%

Structure–activity relationship of immunomodulating pectins from elderberries

Ahmed

Zou

et al. 2015

Carbohydrate Polymers

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“…Glucose uptake assay using 2-NBDG Glucose uptake assay was performed in differentiated L6 cell lines following the method of Chen et al (2010). Cells were pretreated with different concentration of extracts for 24 h. Media was removed, and cells were washed twice with precooled phosphate buffered saline (PBS) replaced with the fluorescent analogue of glucose, 2-NBDG (100 lM) and incubated for 30 min.…”

Section: Glucose Uptake Studymentioning

confidence: 99%

Nutraceutical properties of cumin residue generated from Ayurvedic industries using cell line models

et al. 2016

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Spent cumin (SC), generated from Ayurvedic industry, was evaluated for its nutraceutical potential in terms of antioxidant, antidiabetic and anticancer properties, and compared with that of the raw cumin (RC). SC and RC seeds were extracted with ethyl acetate (E) and methanol (M). SCM (methanol extract) were rich in p-coumaric acid, ferulic acid, ellagic acid and cinnamic acid (6.4445, 5.8286, 2.1519, 4.3085 mg/g dry extract). SCM reduced Fe 2? ion (89.68 lM AA/g dry weight), scavenged DPPH radical (IC 50 -238.6 lg/mL), better a-amylase inhibition (IC 50 -337.22 lg/mL) and glucose uptake activity in 30.7% of L6 cells. SCM inhibited viability, retarded migration area up to 41.02%, arrested cell cycle at S phase and induced apoptosis in 2.45% of HT29 colon cancer cells. The results indicated that dietary interventions using nutraceutical food formulation made out of SC can play a significant role in the prevention and management of degenerative diseases.

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“…Glucose uptake assay by 2-NBDG Glucose uptake assay was performed according to the method of Chen et al (2010). L6 rat skeletal muscle cells were cultured in DMEM with 10 % fetal bovine serum.…”

Section: Mtt Assaymentioning

confidence: 99%

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

2014

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Piper longum L. (Family: Piperaceae), is a widely used herb in several Ayurvedic formulations prescribed for various diseases. Potential of the plant material as an antidiabetic and cardio protective agent has not been evaluated so far. In the study, we designed experiments to evaluate antioxidant, glucose uptake potential and lipid content regulating potential of extracts and compound from P. longum fruits. Solvent extracts from Piper longum fruits using hexane, ethyl acetate, methanol, 70 % methanol-water were taken and apigenin 7, 4'-dimethyl ether (ADE) was isolated from ethyl acetate extract. Antioxidant activity, glucose uptake potential and adipocyte differentiation assay was performed with extract and pure compound. Antioxidant activity in terms of TRP (196.03 μg/mg GAE), DPPH assay (IC 50 -173.09 μg/mL), hydroxyl radical scavenging assay (IC 50 -20.42 μg/mL), inhibiting LDL oxidation (IC 50 -51.99 μg/mL) and to enhance SOD activity (25.3 %) was higher in ethyl acetate extract (EAP). Phenolic and flavonoid content was measured and showed a positive correlation with antioxidant activity. Presence of apigenin 7, 4'-dimethyl ether (ADE) and piperine (Pip) in EAP was determined by HPTLC analysis and was isolated. ADE inhibited α-glucosidase and α-amylase enzymes and enhanced 2-NBDG uptake in L6 cells. Hypolipidemic effect of ADE on mouse pre-adipocyte (3T3L1) cell lines also showed a dose dependent reduction on lipid droplet content and effective concentration range was determined as 1-2.5 μg/ mL. The results suggested that Piper longum fruits can provide a natural source of antioxidants with antidiabetic and anti obesity potential.

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Flavonoids and Isoflavonoids from Sophorae Flos Improve Glucose Uptakein Vitro

Cited by 44 publications

References 22 publications

Structure–activity relationship of immunomodulating pectins from elderberries

Structure–activity relationship of immunomodulating pectins from elderberries

Nutraceutical properties of cumin residue generated from Ayurvedic industries using cell line models

Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4'-dimethyl ether isolated from Piper longum L

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