Flavonoid profile, antioxidant and cytotoxic activity of different extracts from Algerian Rhamnus alaternus L. bark

Boussahel, Soulef; Speciale, Antonio; Dahamna, Saliha; Amar, Yacine; Bonaccorsi, Irene; Cacciola, Francesco; Cimino, Francesco; Donato, Paola; Ferlazzo, Guido; Harzallah, Daoud; Cristani, Mariateresa

doi:10.4103/0973-1296.157707

Cited by 25 publications

(17 citation statements)

References 25 publications

(26 reference statements)

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“…Based on the IC50 value of compounds 2 showed stronger activity, suggesting that the presence of an additional hydroxyl group at C-14 and opening of D-ring of triterpenoid structure seems to increase the cytotoxic activity. These results are similar to those of previously reported that the presence of additional of the hydroxyl group and the opening of D-ring in flavonoid structure can increase the cytotoxic activity (Boussahel et al, 2015).…”

Section: Figure 2 1 H-1 H and Hmbc Correlation Of 1 Andsupporting

confidence: 92%

Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

et al. 2019

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The Garcinia genus is a well known tropical plant in the Indo-Malesiana region and mainly distributed in tropical countries including Indonesia, Thailand, and Malaysia. Previous phytochemical studies on Garcinia species have led to the identification and isolation of mainly prenylated xanthones. This research describes the isolation and structure elucidation of isolated triterpenoids compounds from the bark of Garcinia porecta. Dried powder bark of G. porecta was extracted with methanol and then partitioned with n-hexane, ethyl acetate, and n-butanol. The n-hexane extract then was separated and purified with chromatography techniques to obtain isolated compounds 1 and 2. The chemical structure of isolated compounds was elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis and identified as lanosterol (1) dan arabidiol (2), respectively. These triterpenoids were isolated from this plant for the first time. Compound 1 and 2 showed weak cytotoxic activity against MCF-7 breast cancer cells with IC50 values of 60.09 dan 46.17 µM, respectively.

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Section: Figure 2 1 H-1 H and Hmbc Correlation Of 1 Andsupporting

confidence: 92%

Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

et al. 2019

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“…While ORAC and Folin–Ciocalteu tests are not suitable for measuring liposoluble antioxidants, ABTS can measure the activity of both water-soluble antioxidants and liposoluble ones and DPPH in turn is soluble only in organic solvents [27, 28]. …”

Section: Resultsmentioning

confidence: 99%

Bioactivity and Toxicity of Senna cana and Senna pendula Extracts

Monteiro

Júnior

Oliveira

et al. 2018

Biochemistry Research International

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This work investigated the content of total polyphenolic compounds and flavonoids as well as their toxicity and larvicidal and acetylcholinesterase inhibitory activities. The antioxidant activities of two medicinal Senna species extracts (Senna cana and Senna pendula) were also investigated. The ethanol extract of the leaves of S. cana and the ethanol extract of the branches of S. pendula presented the best performance in the DPPH/FRAP and ABTS/ORAC assays, respectively. For the inhibition of acetylcholinesterase, the hexane extract of the flowers of S. pendula presented the lowest IC50 value among the ethanol extracts of the leaves of S. cana and showed the best performance in some assays. The hexane extract of the leaves of S. pendula and the hexane extract of the branches of S. cana were moderate to Artemia salina Leach. In the quantification of phenols and flavonoids, the ethanol extract of the leaves of S. cana presented the best results. The ethanol extracts of the leaves of S. cana were found to be rich in antioxidants, phenolic compounds, and flavonoids. These results indicate the antioxidant potential of the extracts of Senna species and can be responsible for some of the therapeutic uses of these plants.

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“…[ 19 ] and adapted in our laboratory. [ 20 ] This assay is based on the effect of peroxyl radicals generated from the thermal decomposition of 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) on the signal intensity from the fluorescent probe FL in the presence of an oxygen radical absorbing substance. The reaction was carried out in 75 mM phosphate buffer (pH 7.4), and the final reaction mixture was 2 ml.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and anti-inflammatory properties of Algerian Thymelaea microphylla coss. and dur. extracts

Dehimi¹,

Speciale²,

Saija³

et al. 2016

Phcog Mag

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Background:Thymelaea microphylla Coss. et Dur. (Thymelaeaceae) (TM) is a rare medicinal plant endemic to Algeria. Leaves decoction is used in folk medicine for anticancer, anti-inflammatory, and antidiabetic properties.Objective:Herein, the antioxidant and anti-inflammatory properties of different extracts from leaves and flowers of Algerian TM were evaluated.Materials and Methods:The study was carried out by in vitro cell-free assays (antioxidant/radical properties), ex vivo experiments (inhibition of prostaglandin E2 and thromboxane B2 release in human whole blood) and in vitro experiments on cell systems (cytotoxicity on peripheral blood mononuclear cells, and protective effects on human vein endothelial cells exposed to TNF-α).Results:The acetone TM extract showed significant antioxidant properties and excellent anti-inflammatory and cyclooxygenase-inhibitory activity, together with lack of toxicity on normal human blood cells; furthermore, it was able to protect endothelial cells against dysfunction induced by TNF-α, as shown by decrease in cell death, e-selectin expression and leukocyte adhesion.Conclusion:On these bases, TM leaves and flowers appear to be a good source of bioactive compounds with significant antioxidant and antiinflammatory capability, and potentially effective in prevention and treatment of pathological conditions related to oxidative stress and inflammation, such as endothelial dysfunction.SUMMARY Thymelaea microphylla leaves and flowers are a good source of bioactive compounds with significant antioxidant/free radical scavenger and antiinflammatory activity.The acetone extract from leaves and flowers of Algerian Thymelaea microphylla possesses excellent cyclooxygenase-inhibitory activity.This extract is able to protect against endothelial dysfunction, an early event in development of atherosclerosis and vascular diseases. Abbreviations used: TM: Thymelaea microphylla; BCB: Beta-carotene bleaching; AcE: Acetone extract; PGE2: Prostaglandin E2; TxB2: Thromboxane B2; FL: Fluorescein; Cat: Catechin; DPPH: 2,2-diphenyl-1-picrylhydrazyl; ABTS: 2,2'-azinobis-(3-ethyl-benzothiazolin-6-sulfonic acid)+; Que: Quercetin; ORAC: Oxygen radical absorbance capacity; AAPH: 2,2'-azobis (2-methylpropionamidine)dihydrochloride; PMS/NADH: Phenazine methosulfate/nicotinamide adenine dinucleotide; HUVECs: Human umbilical vein endothelial cells.

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Flavonoid profile, antioxidant and cytotoxic activity of different extracts from Algerian Rhamnus alaternus L. bark

Cited by 25 publications

References 25 publications

Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

Bioactivity and Toxicity of Senna cana and Senna pendula Extracts

Antioxidant and anti-inflammatory properties of Algerian Thymelaea microphylla coss. and dur. extracts

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