Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats

Kobo, Patricia Ishaku; Ayo, Joseph Olusegun; Aluwong, Tagang; Zezi, Abdulkadir Umar; Va, Maikai; Ambali, Suleiman Folorunsho

doi:10.1016/j.rvsc.2013.10.005

Cited by 24 publications

(17 citation statements)

References 23 publications

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“…[33] This has been further buttressed by a significant increase in erythrocyte osmotic fragility and MDA concentration in T. brucei brucei infected Wistar rats. [16] This demonstrates the chemoprotective effects of A. indica on oxidative stress via decrease in lipid peroxidation and enhancement of the antioxidant status. [34] MDA is an end product of the oxidative degradation of lipids.…”

Section: Discussionmentioning

confidence: 92%

“…[131415] However, oxidative stress is due to an imbalance between reactive oxygen species (ROS) and antioxidant defense system activities with a concomitant increase in the formation of oxidative products. [161718] Furthermore, infection caused by T. brucei group of parasites have been shown to alter the antioxidant defense of the host and that pathological processes are initiated through the release of cytokines and nitric oxide. [192021]…”

Section: Introductionmentioning

confidence: 99%

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Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs

et al. 2015

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Introduction:The medicinal properties of Azadirachta indica have been harnessed for many years in the treatment of many diseases in both humans and animals.Materials and Methods:Twenty-five apparently healthy dogs weighing between 3 and 8 kg were randomly divided into five groups with five dogs in each group. Ameliorative effect of A. indica on erythrocyte antioxidant status and markers of oxidative stress were assessed. Liver and kidney function tests were also performed.Results:Pre-treatment with methanolic extract of Azadirachta indica (MEAI) at different doses did not significantly alter the values of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activity in Trypanosoma brucei infection. Although, serum creatinine significantly (P < 0.05) decreased with pre-treatment with 50 mg/kg A. indica, after 2 weeks of T. brucei infection. However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively. Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals. Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei.Conclusion:From this study, MEAI showed significant ability to attenuate oxidative stress and inflammation during experimental T. brucei infection.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs

et al. 2015

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“…The technique is used in the assessment of membrane integrity of animals having erythrocyte membrane abnormalities associated with hereditary spherocytosis, elliptocytosis and stomatocytosis [2], blood infections such as babesiosis [3], trypanosomosis [4][5][6], and anaplasmosis [7], and autoimmune haemolytic diseases [8]. Osmotic fragility of erythrocytes is also affected by xenobiotic, chemical and drug actions [9][10][11][12][13], and physiological factors such as transport stress, nutritional status, hormonal balance, species, breed and sex [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

Igbokwe

2014

Journal of Basic and Clinical Physiology and Pharmacology

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“…Flavonoids found in higher plants have been reported to possess antimicrobial and antitrypanosomal activities (Cordell et al, 2001). Kobo et al (2014) reported protective effect of flavonoids on erythrocytes of rats infected with T. brucei brucei. Since ethyl acetate and aqueous methanol extracts devoid of flavonoids exhibited slightly reduced motility, flavonoid could be said to be responsible for trypanocidal activity in this study.…”

Section: Concentrations (Mg/ml) Pre-patent Period (Days)mentioning

confidence: 99%

Ex vivo trypanostatic effect of stem-bark extracts of Securidaca longipedunculata (Fres. Holl) against Trypanosoma brucei brucei

Tauheed¹,

Сулейман²,

Mamman³

et al. 2016

Afr. J. Biotechnol.

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Current treatment of trypanosomosis in sub-Saharan Africa is associated with widespread inefficiency.There is therefore the need to find more effective drugs against the disease from promising traditional medicinal herbs. This work is aimed at evaluating ex vivo anti-trypanosomal effect of stem-bark extracts of S. longipedunculata against T. brucei brucei. One hundred microlitre of crude methanol, ethyl acetate and aqueous methanol extracts of S. longipedunculata at concentrations of 3 and 6 mg/ml each were mixed with 50 µl of blood containing 8.1 × 10 6 trypanosomes and incubated at 37°C for 90 min. Similarly, diminazene aceturate (10 µg/ml), physiological saline solution (50 µl) and blood (100 µl) containing trypanosomes only served as treated, negative and untreated controls, respectively. Motility of the parasite was monitored under light microscope (×400) at 5 min interval throughout the 90 min observation period. All experiments were done in duplicate. The mixtures were subsequently inoculated into rats that were not previously infected with trypanosomes. Phytochemical screening of the extracts revealed the presence of carbohydrates, cardiac glycosides, saponins, steroid, triterpenes, flavonoids and tannins. However, aqueous and ethyl acetate fractions were devoid of flavonoids. The crude methanol immobilized the parasites within 75 min, while ethyl acetate and aqueous extracts induced slight reduction in motility of the parasite at 90 min of incubation. However, inoculated rats developed infection and succumbed to the infection. It is concluded that the stem-bark of the plant possesses trypanostatic, but not trypanocidal, activity against the parasite.

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Flavonoid mixture ameliorates increase in erythrocyte osmotic fragility and malondialdehyde concentration induced by Trypanosoma brucei brucei-infection in Wistar rats

Cited by 24 publications

References 23 publications

Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs

Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs

Influence of extracellular media’s ionic strength on the osmotic stability of Sahel goat erythrocytes

Ex vivo trypanostatic effect of stem-bark extracts of Securidaca longipedunculata (Fres. Holl) against Trypanosoma brucei brucei

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