Flavonoid C- and O-glycosides from the Mongolian medicinal plant Dianthus versicolor Fisch.

Identification of flavonoid glycosides in Rosa chinensis flowers by liquid chromatography–tandem mass spectrometry in combination with 13C nuclear magnetic resonance

“…Although it was shown that HPLC–NMR technique provided more structural information [18–19] compared with HPLC-MS, the HPLC-MNR instrument is not readily available [20]. Actually, as we noted, structural elucidation for some flavonoid glycosides detected often become tentative due to the lack of support from either NMR data or authentic standards [4, 15–17]. In the case of R. chinensis , given the facts that only limited phytochemical investigation has been carried out so far and many standard compounds are not commercially available, to unequivocally identify the compounds of interest remains a challenge.…”

Two Unusual Methylidenecyclopropane Glucosides from Metaxya rostrata C.Presl

“…3)) The absolute configuration of the glucose was established after hydrolysis, reaction of the sugar with chiral (−)-( R )-butan-2-ol, trimethylsilylation, and GC/MS analysis of the resulting derivative [5] [6], and, consequently, the absolute configuration of the aglycone was deduced as ( R ).…”

“…The determination of the absolute configuration of the sugar was achieved after enzymatic hydrolysis of 1 mg of compound 1 with β-glucuronidase (No. G0751 from Helix pomatia; Sigma-Aldrich, A-Vienna), butylation with (−)-(R)-butan-2-ol, and derivatization with N-methyl-N- (trimethylsilyl)trifluoracetamide according to [5] [6]. GC/MS Analyses were performed on a GCMS-QP 2010 (Shimadzu Corporation, Kyoto, Japan) according to [6].…”

Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

“…Based on the HPLC fingerprint, 26 major flavonoid constituents were selected for the quantitative analysis. The structures of 13 compounds have been elucidated following bioactivity‐guided fractionation in a previous study 7, and are shown in Fig. 2.…”

“…Our investigations have shown that an aqueous extract and a flavonoid‐enriched fraction of D. versicolor cause an increase in bile flow in the isolated perfused rat liver 5. Bioactivity‐guided fractionation led to the isolation and structure elucidation of a number of flavonoids 7. However, to date neither methods for qualitative analysis of these active constituents nor methods to quantify the flavonoids in D. versicolor have been reported.…”