Quantification of flavonoid glycosides in an aqueous extract from the traditional Mongolian medicinal plant Dianthus versicolorFISCH.

Abstract

An HPLC-diode array detection (DAD) method was established in order to investigate dried aerial parts of Dianthus versicolor FISCH. (Caryophyllaceae), a plant used in traditional Mongolian medicine against liver impairment. Aqueous extracts were separated on an Aquasil(®) C(18) column with a linear gradient of acetonitrile (ACN) and water (adjusted to pH 2.8 with formic acid) as the mobile phase. LC-IT-MS facilitated the assignment of 26 flavonoids, among them a series of rare C-glycosylated as well as O-glycosylated derivatives, which are assumed to be the active principles. Quantification was performed and validated using isovitexin-7-O-glucoside (saponarin) as the external standard. The method showed good linear behaviour (r(2) ≥0.9999) over the investigated concentration range (0.007-3.5 mg/mL). The good precision of the method allowed the successful qualitative and quantitative analysis of flavonoid-glycosides in the aqueous extracts prepared from five different D. versicolor samples. Depending on the origin of the samples, the total flavonoid content was found to vary considerably from 0.41 to 3.30% in the aqueous extracts and from 0.07 to 0.57% in the crude drug. In addition, the relative composition of the various flavonoids was found to differ strongly. These results highlight the need for proper quality control for this herbal drug.

HPLC Determination of Flavonoid Glycosides in Mongolian Dianthus versicolorFisch. (Caryophyllaceae) Compared with Quantification by UV Spectrophotometry

“…The correctness of this approach implies the selection of an appropriate detection wavelength at which all constituents are registered equally. The analytes in D. versicolor represent C ‐ and O ‐glycosides of apigenin, luteolin (3′‐hydroxyapigenin), and chrysoeriol (3′‐methoxyapigenin), whose absorption maxima range between 334 nm and 347 nm (Obmann et al , ). Therefore, the detection wavelength for quantification was set at 340 nm.…”

“…Therefore, we performed own investigations and identified C ‐ and O ‐glycosylated apigenin‐, luteolin‐ and chrysoeriol‐derivatives in an aqueous extract of D. versicolor (Obmann et al , ). For these flavonoids, a quantification method by HPLC and external standardisation was developed (Obmann et al , ). It was validated for the aqueous extract, but not for the crude drug, and aimed at the separation of flavones covering a wide polarity range.…”

Anti-inflammatory and uric acid lowering effects of Euodiae fructus on hyperuricemia and gout mice

“…The UPLC-MS analysis method has been described previously (Obmann et al, 2011). Briefly, the metabolites in the ERE were identified using a Waters ACQUITY UPLC I-Class (Waters, Milford, MA, United States), a Waters ACQUITY UPLC BEH-C18 column (2.1 × 100 mm, 1.8 μm), and a mass spectrometer (Waters XEVO G2-QTOF).…”

The Neuroprotective Effect of the Extract of Traditional Mongolian Medicine Eerdun Wurile in PC12 Cells

“…The extract was prepared by refereeing the method in literature [10,11]. The Eerdun Wurile pills were ground very fine powder.…”