HPLC Determination of Flavonoid Glycosides in Mongolian Dianthus versicolorFisch. (Caryophyllaceae) Compared with Quantification by UV Spectrophotometry

Abstract

The study has indicated that the newly developed HPLC method represents a powerful technique for the quality control of D. versicolor. Ultraviolet spectrophotometry may be used alternatively provided that the less polar flavonoids are removed by purification.

Evaluation of 5-fluorouracil released from a foldable capsular vitreous body in vitro and in vivo

“…As there is a lack of qualitative measurement in the UV spec, adjuvants which exist both in 5-Fu injection and sterile water result in a higher dosage of released 5-Fu. The UPLC method represents a powerful technique for qualitative measurement, and shows high accuracy, precision and selectivity for the determination without any interference from the excipients [30]. In addition, we attempted to mimic the in-vivo conditions; however, there was a lack of the constant circulation of aqueous humour and blood that would, in in-vivo conditions, rapidly clear the contents released from the FCVB, which restricted the total release rate and resulted in the lower concentration of released drug.…”

Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

“…According to the available literature, virtually all mobile phases used for analysis of luteolin derivatives consisted either of ACN/H 2 O, e.g., [26,66,67,78] or, less often, MeOH/H 2 O mixtures [72,79,80,81]. Additionally, a modifier such as FA, e.g., [79,82,83,84], AcOH, e.g., [44,78,85,86,87], or trifluoroacetic acid (TFA) [88] was added to avoid peak tailing [89]. Analyses performed without acidic modifiers are rare [90].…”

“…By far, the most common method for detection of luteolin derivatives is using diode array detectors (DADs) (also called photodiode array detectors, PDA), which were used in as many as 46 instances (e.g., for the analysis of luteolin derivatives and other flavonoids in Cymbopogon citratus [79], Dianthus versicolor [88], Clinacanthus nutans [44], Thymus alternans and others [93]). More often than not, however, DADs were combined with other detectors for additional structure determination or confirmation, with or without prior isolation.…”

Determination of eleven flavonoids in chamomile and linden extracts by capillary electrophoresis

“…So far, analysis of avonoids has been accomplished predominately by high performance liquid chromatography (HPLC) [11][12][13][14] and gas chromatography (GC). 15,16 There have been capillary electrophoretic and micellar electrokinetic chromatography methods for the separation of selected avonoids reported.…”