Flavobacterium ustbae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia

Liu, Yang; Han, Ho Le; Zou, Yuanyuan; Kim, Song‐Gun

doi:10.1099/ijsem.0.003717

“…To determine the fatty acid composition, cells of strain PL0132 T and the reference strains were collected from R2A agar at the same physiological age, by using the method of Sasser 42 . Fatty acid analyses were analyzed by the Sherlock Microbial Identification System (TSBA; library version 6.0) 43 . The isolated strain was cultured in R2A broth (Difco, BD) for 48 h to collect cell mass for isoprenoid quinone and polar lipid analysis.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique

Han,

Nurcahyanto,

Muhammad

et al. 2023

Sci Rep

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In the effort of isolating novel microbial species, the strain PL0132T was isolated from a fallen leaf under fresh water at a stream, which glided when grown on a tap water medium (without nutrients). The strain was determined to be Gram-negative, strictly aerobic, and rod-shaped, which grew optimally at 25 °C, pH 6–7, and the strain tolerates 1% (w/v) NaCl concentration. The complete genome of strain PL0132T comprises one contig with a sequencing depth of 76×, consisting of 8,853,064 base pairs and the genomic DNA G + C content was 46.7% (genome). 16S rRNA gene sequence analysis revealed that strain PL0132T represents a member of the phylum Bacteroidetes and is affiliated with the genus Spirosoma. Based on genomic, phenotypic, and chemotaxonomic characteristics, the strain PL0132T represents a novel species of the genus Spirosoma, for which the name Spirosoma foliorum sp. nov. is proposed (= KCTC 72228 T = InaCC B1447T).

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“…In general, the cells in genus Flavobacterium are Gram-stain-negative, rod-shaped, aerobic and non-spore-forming. Additionally, their chemotaxonomic characteristics consist of phosphatidylethanolamine as a major polar lipid and MK-6 as the only respiratory quinone [7, 11]. The DNA G+C contents of the genus Flavobacterium are between 30–52 % [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium litorale sp. nov., isolated from red alga

Muhammad

¹

,

Han

²

,

Lee

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative and rod-shaped bacterial strain (WSW3-B6T) was isolated from red alga collected from the West Sea, Republic of Korea. Cells of strain WSW3-B6T were non-motile, aerobic and produced slightly yellow and mucoid colonies on marine agar. The strain grew optimally at 23–30 °C, with 0.5–4 % NaCl (w/v) and at pH 6.5–8.5. A phylogenetic analysis of the 16S rRNA gene revealed that strain WSW3-B6T belongs to the genus Flavobacterium within the family Flavobacteriaceae , having the highest sequence similarity to Flavobacterium arcticum SM1502T (96.7%), followed by Flavobacterium salilacus subsp. altitudinum LaA7.5T (96.2%) and Flavobacterium salilacus subsp. salilacus SaA2.12T (96.2%). The complete sequence of a circular chromosome of strain WSW3-B6T determined by combination of Oxford Nanopore and Illumina platforms comprised a total 2 725 095 bp with G+C content of 37.1 mol%. A comparative analysis based on the whole genome also showed the distinctiveness of strain WSW3-B6T. The average nucleotide identity (ANI) values between strain WSW3-B6T and the closest strains F. arcticum SM1502T, F. salilacus subsp. altitudinum LaA7.5T and F. salilacus subsp. salilacus SaA2.12T were 78.3, 77.8 and 77.7 %, respectively, while the digital DNA–DNA hybridization (dDDH) values between strain WSW3-B6T and the above closely related strains were 21.0, 20.4 and 20.3 %, respectively. Both the ANI and dDDH values supported the creation of a new species in the genus Flavobacterium . The major fatty acids (>10 %) were iso-C15 : 0 (19.3 %), C16 : 0 (14.0 %), iso-C17 : 0 3-OH (13.1 %) and C18 : 0 (10.7 %). The polar lipids of strain WSW3-B6T included phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. Moreover, MK-6 was the only respiratory quinone. A comparison of the phylogenetic distinctiveness and the unique phenotypic and chemotaxonomic characteristics among strain WSW3-B6T and closely related type strains supported that strain WSW3-B6T (=KCTC 82708T=GDMCC 1.2627T) represents a novel species of the genus Flavobacterium , for which the name Flavobacterium litorale sp. nov. is proposed.

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“…The species of this genus are characterized by the presence of phosphatidylethanolamine, menaquinone-6 (MK-6) and iso-C 15 : 0 alongside summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω7c) as the principal polar lipid, predominant respiratory quinone and common major fatty acids, respectively, and DNA G+C contents of 29.9-48.9 mol%. They have been isolated from various habitats, such as glacier ice, seawater, freshwater and plant rhizospheres [4][5][6][7][8], as well as algal [9,10] or cyanobacterial cultures [11,12]. Results from many studies have revealed that species of the genus Flavobacterium become abundant during cyanobacterial blooms, particularly within cyanobacteria aggregates [13,14].…”

Section: Introductionmentioning

confidence: 99%

Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa

Kim

¹

,

Lee

²

,

Lee

³

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A novel aerobic bacterial strain, designated MK012T, was isolated from the phycosphere of Microcystis aeruginosa . MK012T appears to be Gram-stain-negative, non-motile and rod-shaped and has yellow pigments. Phylogenetic analysis using 16S rRNA revealed that this bacterium was most closely related to Flavobacterium buctense T7T (=JCM 30750T; 97.5 %), Flavobacterium dankookense ARSA-19T (=KCTC 23179T; 97.5 %) and Flavobacterium macrobrachii an-8T (=DSM 22219T; 97.3 %). The genome size and genomic DNA G+C content of MK012T were estimated at 3.3 Mbp and 37.6 mol%, respectively. The average nucleotide identity (ANI), average amino acid identity (AAI) and in silico DNA–DNA hybridization (dDDH) values of MK012T and the members of the genus Flavobacterium were found to be 71.8–78.3 %, 65.1–79.6% and 19.1–21.5 %, respectively. MK012T exhibited oxidase but no catalase activity. The cells grew at 14–36 °C (optimum, 25 °C), pH 5–10 (optimum, pH 7) and 0–0.4 % (w/v) NaCl (optimum, 0 % NaCl) in R2A medium. MK012T did not produce flexirubin-type pigments. The predominant cellular fatty acids of MK012T were determined to be iso-C15 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, and summed feature 9 (10-methyl C16 : 0 and/or iso-C17 : 1ω9c); MK-6 was identified as its only respiratory quinone. Its major polar lipids were determined to be phosphatidylethanolamine, two unidentified aminolipids, and two unidentified polar lipids. The genotypic and phenotypic characteristics indicate that MK012T (=KACC 21509T=JCM 33879T) represents a novel species of the genus Flavobacterium ; therefore, the name Flavobacterium phycosphaerae sp. nov. is proposed.

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Flavobacterium ustbae sp. nov., isolated from rhizosphere soil of Alhagi sparsifolia

Cited by 10 publications

References 27 publications

Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique

Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique

Flavobacterium litorale sp. nov., isolated from red alga

Flavobacterium phycosphaerae sp. nov. isolated from the phycosphere of Microcystis aeruginosa

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