Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants

Batista, Diego Silva; Dias, Leonardo Lucas Carnevalli; Rêgo, Maílson Monteiro do; Saldanha, Cleber Witt; Otoni, Wagner Campos

doi:10.1590/0103-8478cr20150245

Cited by 33 publications

(10 citation statements)

References 22 publications

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“…Considering the susceptibility of peppers to diseases and pests, the improvement of tissue An Acad Bras Cienc (2021) 93(3) e20190061 2 | 15 culture techniques is very important. However, some authors report recalcitrance of the genus Capsicum for in vitro regeneration, which may hinder the applicability of genetic improvement techniques and molecular biology (Kothari et al 2010, Mohamed & Alsadon 2011, Batista et al 2017, Gammoudi et al 2018). In addition, some authors report that this recalcitrance has a positive correlation to ethylene levels produced during in vitro growth and development, making it a limiting factor for species such as pepper (Santana-Buzzy et al 2006, Batista et al 2013, Gammoudi et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars

Gris

Pinheiro

Thiesen

et al. 2021

An. Acad. Bras. Ciênc.

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The aim of this work was to evaluate the effect of fi ve light qualities [fl uorescent lamps (FL); white (WL), red (RL), blue (BL), or red/blue LEDs (RBL)] and two types of fl ask sealing (with or without porous membranes) in the in vitro germination and growth of two Capsicum frutescens cultivars (Espaguetinho and Cayenne). For this, two seeds were inoculated per fl ask (350 mL) containing 50 mL of MS medium with 100 mg L -1 myo-inositol, 30 g L -1 sucrose, 6.0 g L -1 agar, and pH 5.8 ± 0.1. The plants were kept under culture room conditions (16-h photoperiod, 25 ± 2°C and irradiance of 40 μmol m -2 s -1 ).The experiment was arranged in CRD, 2x5x2 factorial scheme (Cultivar X Light qualities X Sealing type). At 42 days, the following traits were evaluated: percentage of germination; root and shoot length; number of green leaves, senescent leaves and nodal segments; and fresh and dry weight. Plants grown under RL and BRL, showed greater number of green leaves, and longer shoots and roots. The use of porous membranes remarkably reduced the number of senescent leaves. Thus, the use of porous membranes, RL and BRL are recommended since they improve growth and development of pepper in vitro.

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Section: Introductionmentioning

confidence: 99%

Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars

Gris

Pinheiro

Thiesen

et al. 2021

An. Acad. Bras. Ciênc.

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“…However, in vitro propagation with the use of porous membranes allows the exchange of gases between the external and internal atmosphere of the flasks through natural ventilation, provided that the concentration of CO 2 is adequate, resulting in increased growth (Kozai, 2010;Martins et al, 2015). In Capsicum annuum, Batista et al (2017) verified higher shoot length and number of leaves per explant in membrane system when compared to conventional system. For Vaccinium ashei Reade, the membrane-based system provided higher shoot length, number of shoots, leaf area and chlorophyll content (Hung et al, 2016).…”

Section: Mixotrophism Effect On In Vitro Elongation and Adventitious mentioning

confidence: 88%

“…Controlling environmental factors such as the use of mixotrophism is considered important for in vitro morphogenetic response (Heringer et al, 2017). Generally, a positive response to micropropagation among the various factors is due to the reduction or exclusion of the sucrose source, as well as the use of porous membranes, in which the relative humidity of the bottles, characterized by the presence of water and nutrients in the plant, is reduced (Bacillus et al, 2005), thus improving its ex vitro acclimatization process (Saldanha et al, 2012;Jiménez et al, 2015;Batista et al, 2017;Tisarum et al, 2018). Silva et al (2017) reported its positive effect in different species, besides the possibility of variations in the concentration of sucrose, which benefits the cultivation and reduces expenses.…”

Section: Introductionmentioning

confidence: 99%

MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Souza

Fernandes

Avelar

et al. 2019

CERNE

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“…The results obtained highlight the importance of the type of flask used and, therefore, the internal atmosphere of the same on the performance of the cultures and their relationship with the disinfestation effectiveness by CB. At the tissue culture of several plants species the morphogennic responses have been associated to the quality of the internal culture and the free volume of air (head space) in the flasks, the type and size of flasks and the type of seal (Demeester et al 1995;Chen 2004;Marino and Berardi 2004;Zobayed 2006;Gonç alves et al 2008;Ribeiro et al 2009;Rodrigues et al 2012;Pinheiro et al 2013;Srivastava et al 2016;Batista et al 2017). …”

Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

Fontes¹,

Álvarez²,

Novais³

et al. 2017

JAS

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The use of a commercial bleach solution (CB) for in vitro propagation of orchids has proven to be an efficient technique and an alternative to the use of the autoclave and laminar flow chamber (LFC). The aim of this study was to evaluate the use of CB in disinfestation of culture media (CM) for seeding and for re-cultivation of orchid plantlets and its effect in different types of flasks and on the nutritional state of the plantlets. In the seeding experiment, seeds of Laelia tenebrosa Rolfe were used, with evaluation of three types of flasks; five concentrations of CB applied to the CM of 2, 5, 10, 20 and 40 mL L -1 and the control treatment. In the re-cultivation experiment, Cattleya kerri Brieger & Bicalho plantlets were used. The rates of CB added to the CM were 2, 5, 10, 20, 30, 35 and 38 mL L -1 in polypropylene containers, and the rates sprayed on the plantlets at re-cultivation were 5, 12.5, 25, 50, 75, 87.5 and 95 mL L -1 . The proportion of germinated seeds and the dry matter weight of the protocorms were greater in the control treatment. The increasing rates of CB added to the CM had worse results for germination in glass flasks. In re-cultivation, there was no significant difference in the growth of the plantlets between the autoclaved flasks and those disinfested in CB.Keymessage: Both orchid seeding and re-cultivation of seedlings can be carried out entirely and efficiently without the use of an autoclave and laminar flow chamber, using chemical disinfection with commercial bleach.

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Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants

Abstract: Flask sealing on in vitro seed germination and morphogenesis of two types of ornamental pepper explants.Ciência Rural, v.47, n.3, 2017.

Cited by 33 publications

References 22 publications

Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars

Light quality and sealing type affect in vitro growth and development of Capsicum frutescens cultivars

MIXOTROPHISM EFFECT ON IN VITRO ELONGATION AND ADVENTITIOUS ROOTING OF Eucalyptus dunnii

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

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