Flame Response in the Quantitative Determination of High Molecular Weight Paraffins and Alcohols by Gas Chromatography.

Perkins, Gerald; Laramy, R. E.; Lively, L. D.

doi:10.1021/ac60196a028

Cited by 53 publications

(23 citation statements)

References 3 publications

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“…Furthermore, high sensitivity, a broad linear range (typically 2000-0.4 μg/mL), and the expectation that compounds of similar structure (carbon percentage and functionality) will elicit a similar response, making the FID the detector of choice [3][4][5][6][7][8]. In our experience, the majority of samples have impurities eluting within ±1 min of the main analyte, supporting the assumption of 'like structure' which is in line with our original working model [9].…”

Section: Gas Chromatography With Flame Ionisation Detectionmentioning

confidence: 99%

“…Gas chromatography (GC) and highperformance liquid chromatography (HPLC) provide a means of separating the impurities from the main component (usually the analyte of interest), and the use of a suitable detector facilitates their quantification. Flame ionisation detection (FID) interfaced with GC provides a measure of carbon, making it ideal for the analysis of volatile organic compounds [3][4][5][6][7][8]. Analytes more suited to liquid chromatography are detected and quantified by way of UV/Vis spectroscopy (UV), and for those not possessing a suitable chromophore, evaporative light scattering detector (ELSD) and charged aerosol detector (CAD) are applicable.…”

Section: Introductionmentioning

confidence: 99%

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The development of an efficient mass balance approach for the purity assignment of organic calibration standards

et al. 2015

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The purity determination of organic calibration standards using the traditional mass balance approach is described. Demonstrated examples highlight the potential for bias in each measurement and the need to implement an approach that provides a cross-check for each result, affording fit for purpose purity values in a timely and cost-effective manner. Chromatographic techniques such as gas chromatography with flame ionisation detection (GC-FID) and high-performance liquid chromatography with UV detection (HPLC-UV), combined with mass and NMR spectroscopy, provide a detailed impurity profile allowing an efficient conversion of chromatographic peak areas into relative mass fractions, generally avoiding the need to calibrate each impurity present. For samples analysed by GC-FID, a conservative measurement uncertainty budget is described, including a component to cover potential variations in the response of each unidentified impurity. An alternative approach is also detailed in which extensive purification eliminates the detector response factor issue, facilitating the certification of a super-pure calibration standard which can be used to quantify the main component in less-pure candidate materials. This latter approach is particularly useful when applying HPLC analysis with UV detection. Key to the success of this approach is the application of both qualitative and quantitative (1)H NMR spectroscopy.

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Section: Gas Chromatography With Flame Ionisation Detectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The development of an efficient mass balance approach for the purity assignment of organic calibration standards

et al. 2015

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“…Nonanoic acid methyl ester (3.5 mM) was added to the assay mixture before acidification from a 100 mM stock solution in DMSO to serve as an internal standard. Quantitation of the products, for which no authentic standards were available, was achieved by comparison with the internal standard and correction for differences in FID detector response (Perkins et al, 1963). Rates were calculated from the amount of products (sum of 12-, 13-, and 14-hydroxypentadecanoic acids) formed per unit time.…”

Section: Gas Chromatography Of Fatty Acid Metabolitesmentioning

confidence: 99%

Controlled regioselectivity of fatty acid oxidation by whole cells producing cytochrome P450BM-3 monooxygenase under varied dissolved oxygen concentrations

Schneider

Wubbolts

Oesterhelt

et al. 1999

Biotechnol. Bioeng.

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“…On one hand, some studies have considered the effect of heteroatoms and functional groups on the response factor and have shown that the effective carbon number for these substituted compounds may be different than the unsubstituted hydrocarbon (Perkins et al, 1962(Perkins et al, , 1963Mockel, 1976;Jorgensen et al, 1990;Huang et al, 1990;Kallai et al, 2001). In other words, a hydrocarbon containing heteroatoms or functional groups may exhibit a different FID response factor than the unsubstituted hydrocarbon.…”

Section: Introductionmentioning

confidence: 99%

Simplified Analysis of Chlorinated Ethenes in Water Samples by Gas Chromatography with Flame Ionization Detection

Ticknor¹,

Elsayed-Ali²,

Kim³

et al. 2013

Environmental Engineering Science

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Chlorinated ethenes are among the most common environmental contaminants and are known or suspected carcinogens. This class of compounds includes perchloroethene (PCE), trichloroethene (TCE), and their breakdown products, including dichloroethene (DCE) isomers and vinyl chloride (VC). Engineers and scientists must be able to measure concentrations of these chemicals in water samples to assess site contamination, monitor clean-up progress, and test possible remediation technologies. Gas chromatography with flame ionization detection (GC/ FID) is a common method for measuring these contaminants in environmental samples. In this study, we tested the hypothesis that FID response factors are equal for all chlorinated ethene compounds. The rationale for the investigation was that if the hypothesis is correct, a single calibration curve can be used for GC/FID analysis of all chlorinated ethene compounds, saving time and money during sample analysis. Based on our measurements, a single calibration curve (FID response versus mass of analyte injected) is applicable to analysis of PCE, TCE, and all three DCE isomers (r 2 = 0.990, n = 50 measurements), allowing for simplified quantification of those chemicals. However, the apparent FID response factor for VC was lower by *40%, indicating that a separate calibration curve would need to be used to accurately estimate the VC concentration in water samples. The difference in the apparent VC response factor is caused predominantly by losses of VC to volatilization during the analysis.

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Flame Response in the Quantitative Determination of High Molecular Weight Paraffins and Alcohols by Gas Chromatography.

Cited by 53 publications

References 3 publications

The development of an efficient mass balance approach for the purity assignment of organic calibration standards

The development of an efficient mass balance approach for the purity assignment of organic calibration standards

Controlled regioselectivity of fatty acid oxidation by whole cells producing cytochrome P450BM-3 monooxygenase under varied dissolved oxygen concentrations

Simplified Analysis of Chlorinated Ethenes in Water Samples by Gas Chromatography with Flame Ionization Detection

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