Flagellin Enhances Saliva IgA Response and Protection of Anti-caries DNA Vaccine

Shi, Wanliang; Li, Y.H.; Liu, F.; Yang, Jingyi; Zhou, Demin; Chen, Y.Q.; Zhang, Yating; Yang, Ying; He, Benxia; Chen, Han; Fan, M.W.; Yan, Huimin

doi:10.1177/0022034511424283

Cited by 25 publications

(39 citation statements)

References 30 publications

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“…This group demonstrated chitosan could increase salivary IgA antibody responses as adjuvants to a DNA vaccine of pGJA-P/VAX [12]. We sought to further enhance salivary IgA and serum IgG antibody responses by including Salmonella-derived flagellin protein at the time of pGJA-P/VAX administration [11,20]. We found that recombinant flagellin protein as a mucosal adjuvant for anti-caries DNA vaccine pGJA-P/VAX via intranasal immunization can increase serum PAc-specific IgG and saliva PAc-specific IgA antibody responses as compared with pGJA-P/VAX alone and significantly decrease the colonization of S. mutans on rat teeth.…”

Section: Enhancement Of Specific Anti-caries Salivary Siga Antibodiesmentioning

confidence: 99%

“…More importantly, flagellin protein as a mucosal adjuvant promoted the ability of pGJA-P/VAX to reduce caries lesions, providing effective protection against dental caries. It is demonstrated that recombinant flagellin protein as a mucosal adjuvant for intranasal caries DNA vaccine enhanced saliva IgA response and conferred better protection against caries [20].…”

Section: Enhancement Of Specific Anti-caries Salivary Siga Antibodiesmentioning

confidence: 99%

“…We applied the recombinant flagellin as mucosal adjuvant in anti-caries vaccines by nasal immunization, either using flagellin directly by simple mixing with antigen or using fusion strategy to combine flagellin and the target antigen in a single fusion protein, proved the efficacy for enhancement of specific IgA response in oral fluids and better protection against caries [11,20,21]. Here, I review some of the main strategies that have been used for successful mucosal vaccination of caries vaccine and discuss the importance of the mucosal adjuvant choice for achieving protective immunity at oral mucosal membranes.…”

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confidence: 99%

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Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Yan

2013

Sci. China Life Sci.

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Dental caries remains one of the most common global chronic diseases caused by Streptococcus mutans, which is prevalent all over the world. The caries prevalence of children aged between 5-6 years old in China is still in very high rate. A potent and effective anti-caries vaccine has long been expected for caries prevention but no vaccines have been brought to market till now mainly due to the low ability to induce and maintain protective antibody in oral fluids. This review will give a brief historical retrospect on study of dental caries and pathogenesis, effective targets for anti-caries vaccines, oral immune system and immunization against dental caries. Then, salivary IgA antibodies and the protective responses are discussed in the context of the ontogeny of mucosal immunity to indigenous oral streptococcal. The methods and advancement for induction of specific anticaries salivary sIgA antibodies and enhancement of specific anti-caries salivary sIgA antibodies by intranasal immunization with a safe effective mucosal adjuvant are described. The progress in the enhancement of salivary sIgA antibodies and anticaries protection by intranasal immunization with flagellin-PAc fusion protein will be highlighted. Finally, some of the main strategies that have been used for successful mucosal vaccination of caries vaccine are reviewed, followed by discussion of the mucosal adjuvant choice for achieving protective immunity at oral mucosal membranes for development of a nasal-spray or nasal-drop anti-caries vaccine for human. mutans streptococci, secretory IgA, PAc, mucosal immunization, caries vaccine Citation:Yan H M. Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine.

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Section: Enhancement Of Specific Anti-caries Salivary Siga Antibodiesmentioning

confidence: 99%

Section: Enhancement Of Specific Anti-caries Salivary Siga Antibodiesmentioning

confidence: 99%

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confidence: 99%

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Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Yan

2013

Sci. China Life Sci.

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“…16,17 Recently, alternatives that might offer the possibility for efficient reduction of cariogenic microorganisms and, consequently, make a feasible control of dental caries are being sought. [18][19][20][21] Under these circumstances, photodynamic therapy (PDT) may be a possible new approach for caries treatment.…”

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confidence: 99%

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Araújo

Fontana

Bagnato

et al. 2012

Photomedicine and Laser Surgery

100

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Background data: The presence of Streptococcus mutans and Lactobacillus acidophilus in dental structure is an indicator of a cariogenic biofilm. Photodynamic therapy is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals capable of inducing cell death. Reduction of bacteria levels can provide additional means of preventing dental caries. Objective: The present study evaluated the susceptibility of planktonic cultures of S. mutans (ATCC 25175) and L. acidophilus (ATCC-IAL-523) from the Adolfo Lutz Institute (IAL) to photodynamic therapy after sensitization with curcumin and exposure to blue light at 450 nm. Methods: Bacterial suspensions of S. mutans and L. acidophilus isolated (as single species) and combined (multspecies) were prepared and then evaluated. Four different groups were analyzed: L-D-(control group), L-D + (drug group), L + D-(light group), and L + D + (photodynamic therapy group). Two different concentrations of curcumin were tested (0.75 and 1.5 g/L) associated with a 5.7 J/cm 2 light emission diode. Results: Significant decreases ( p < 0.05) in the viability of S. mutans were only observed when the bacterial suspensions were exposed to both curcumin and light. Then, reductions in viability of up to 99.99% were observed when using 1.5 g/L of the photosensitizer. The susceptibility of L. acidophilus was considerably lower (21% and 37.6%) for both curcumin concentrations. Conclusions: Photodynamic therapy was found to be effective in reducing S. mutans and L. acidophilus on planktonic cultures. No significant reduction was found for L-D + , proving the absence of dark toxicity of the drug.

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“…Efforts have been made to develop antibacterial dental materials to combat bacteria and secondary caries. Several bioactive agents have been synthesized and incorporated into dental materials [Cheng et al, , 2013Huang et al, 2012;Shi et al, 2012], including various quaternary ammonium monomers (QAMs) that demonstrate favorable antibacterial properties.…”

Section: Evaluation Of Novel Anticaries Adhesive In a Secondary Cariementioning

confidence: 99%

Evaluation of Novel Anticaries Adhesive in a Secondary Caries Animal Model

et al. 2017

Caries Res

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(1) secondary caries was successfully produced in rats; (2) there was a correlation between the modified Keyes scoring method and micro-CT in the evaluation of the secondary caries; (3) the adhesive containing DMADDM significantly reduced both LD and ML (according to micro-CT), and also lowered the scores (based on the modified Keyes scoring method). This suggests that the novel DMADDM adhesive could perform an anticaries function in vivo via the secondary caries animal model which was also developed and testified in research. Secondary caries is one of the major reasons leading to the failure of caries restoration treatment. As a solution, anticaries adhesives perform well in biofilm inhibition in vitro. However, the lack of secondary caries animal models limits the evaluation of anticaries adhesives in vivo. Secondary caries is one of the main reasons for restoration failure with a heavy economic burden [Sakaguchi, 2005;Kasraei et al., 2017]. While many factors facilitate the development of secondary caries, oral bacteria and acid production are the initiators of dental caries [Mjor Keywords Antibacterial material · Bonding system · Dimethylaminododecyl methacrylate · Keyes animal model · Micro-CT · Tooth restoration AbstractWe investigated the anticaries properties of an adhesive containing dimethylaminododecyl methacrylate (DMADDM) in vivo via a secondary caries animal model. Cavities were prepared in the maxillary first molars of Wistar rats. DMADDM-containing adhesives were applied on one side and commercial adhesives on the opposite side as a control. After a 3-week feeding period to induce secondary caries, the molars were harvested for the evaluation of the secondary caries. Lesion depth (LD) and mineral loss (ML) were measured via a micro-CT method, and a modified Keyes scoring method yielded scores for the caries lesions. Statistical analysis was divided into 2 parts: a correlation analysis between 2 evaluations with one-way ANOVA and a least-significant differences (LSD) test, and an evaluation of anticaries adhesives with a paired samples t test. The results showed that:

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Flagellin Enhances Saliva IgA Response and Protection of Anti-caries DNA Vaccine

Cited by 25 publications

References 30 publications

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Salivary IgA enhancement strategy for development of a nasal-spray anti-caries mucosal vaccine

Photodynamic Effects of Curcumin Against Cariogenic Pathogens

Evaluation of Novel Anticaries Adhesive in a Secondary Caries Animal Model

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