Fish oil, contained in eicosapentaenoic acid and docosahexaenoic acid, attenuates testicular and spermatological damage induced by cisplatin in rats

Abstract

The aim of this study was to investigate the beneficial effects of the fish oil (FO) supplementation on oxidative stress, sperm characteristics and histological alterations in the male reproductive system of rats against cisplatin (CP) toxicity. The rats were divided randomly into 4 equal groups (control, FO, CP and FO + CP). FO was orally administered at the dose of 1 softgel per rat per day for 14 days and CP was intraperitoneally given at the dose of 7 mg kg(-1) with a single injection. In CP + FO group, they were applicated at the same doses and times. The results showed that CP caused a significant oxidative damage via induction of lipid peroxidation and reduction in the antioxidant defence system potency in the testis tissue. In addition, sperm motility and sperm concentration significantly decreased but the abnormal sperm rate and histopathological testicular damage increased with CP treatment. On the other hand, FO treatment prevented oxidative, histopathological and spermatological effects of CP and reversed side effects of CP. In conclusion, FO supplementation had significant beneficial effects against CP toxicity on male reproductive system and toxic effects of CP can be prevented by FO treatment. Therefore, it appears that fish oil may be useful for the prevention and treatment of cisplatin-induced reproductive system toxicity.

Combinational treatment of all‐trans retinoic acid (ATRA) and bisdemethoxycurcumin (BDMC)‐induced apoptosis in liver cancer Hep3B cells

“…AB means ATRA combined with BDMC Aydin, Kaya, & Oguz, 2014;Ilbey et al, 2009;Tuglu et al, 2015). Hep3B cells (2 × 10 5 cells/well) were treated with 50 μM of ATRA or with 15 μM of BDMC or their combination for 48 hr.…”

“…# p < .05 was significant difference compared with ATRA only or BDMC only group. AB means ATRA combined with BDMC Aydin, Kaya, & Oguz, 2014;Ilbey et al, 2009;Tuglu et al, 2015).…”

Favourable effect of β‐glucan treatment against cisplatin‐induced reproductive system damage in male rats

“…The high polyunsaturated membrane lipid content of the testes makes the testes more susceptible to the effects of oxidative agents such as CP (Gevrek & Erdemir, ). The protective effect of various natural substances, including fish oil (Ciftci et al, ), curcumin (Gevrek & Erdemir, ), resveratrol (Reddy et al, ), hesperidin (Kaya et al, ) and rutin (Aksu et al, ), against CP‐induced reproductive system damage has also been demonstrated in many studies. To our knowledge, the potential beneficial effects of β‐glucans against CP‐induced reproductive toxicity have not been studied previously.…”

“…Cisplatin (CP) is a chemotherapeutic agent that widely used for many different types of cancer, such as lung, stomach, ovarian and testicular (Ciftci, Cetin, Aydin, Kaya, & Oguz, ; Kaya, Ciftci, Cetin, Doğan, & Başak, ). The clinical usage of CP is restricted due to its severe dose‐dependent side effects (Bayindir et al, ; Eid, Abdelkader, Abd El‐Raouf, Fawzy, & El‐Denshary, ).…”

“…Previous research showed that CP treatment caused damage to many tissues and organ systems, such as reproductive toxicity (Kaya et al, ), nephrotoxicity (Masuda, Inoue, Miyata, Mizuno, & Nanba, ), hepatotoxicity (Ciftci, Onat, & Cetin, ), ototoxicity (Bayindir et al, ), ocular toxicity (Taşlı et al, ) and neurotoxicity (Kaya, Ciftci, Cetin, Tecellioğlu, & Başak, ). Many previous studies on CP‐induced male reproductive system toxicity demonstrated that CP administration led to oxidative damage and apoptosis in testicular tissue, a decline in testes' weight, an increase in abnormal sperm rate and a decrease in sperm concentrations and motility (Aksu et al, ; Ciftci et al, ; Kaya et al, ; Saral et al, ). An imbalance between the reactive oxygen species and the antioxidant defence system of a tissue is defined as oxidative stress that cause lipid peroxidation and the inactivation of many enzymes (Bentli, Ciftci, Cetin, & Otlu, ).…”