“…Similarly, the arginine requirement for flounder larvae seemed to be slightly higher than 3.2% of the CP54 diet due to estimated protein requirement of 55.4% in this study. The nutritional requirements of marine fish larvae have focused primarily on the fatty acid requirements since a few decades ago (Izquierdo, Socorro, Arantzamendi & Hernadez-Cruz, 2000;Kanazawa, 2003), but the AA requirements in recent years (Hamre et al, 2013;Li et al, 2013;Saavedra et al, 2015;Canada et al, 2016). In particular, histidine appeared to be the limiting AA in live food (enriched rotifers and Artemia nauplii) and in dry feed tested for meagre (Agryrosomus regius) larvae when essential AA profiles of fish carcass and diet were compared (Saavedra et al, 2015) or in enriched rotifers when they were fed to Diplodus puntazzo larvae at 4 DAH (Saavedra et al, 2007).…”