First Report of Molecular Identification of Prototheca zopfii Genotype 2 as Causative Agent of Bovine Mastitis in Navsari, South Gujarat, India

Abstract

Prototheca species is emerged as an important causative agent for bovine mastitis worldwide, and is responsible for great economic losses for dairy industry. A 5-year-old HF cow was referred to the Veterinary Clinical Complex of the College, Navsari (India) with a complaint of watery milk with flakes, anorexia and drop in milk production. On physical examination, udder appeared warm and swollen, while milk consistency was thin, watery with flakes. Milk sample obtained aseptically from the cow was inoculated aerobically on Sabouraud dextrose agar and blood agar plates. Prototheca colonies grew in pure and luxuriant form from mastitic milk on both the plates after 48 h of incubation. Prototheca zopfii was identified on the basis of colony morphology, staining characters and biochemical reactions. Molecular confirmation of Prototheca zopfii genotype 2 was performed by employing polymerase chain reaction (PCR). This report presents first isolation and molecular identification of Prototheca zopfii genotype 2 from a case of bovine mastitis from Navsari region, South Gujarat, India.

Prevalence of canine infectious diseases in Gujarat, India: A brief review

“…Canine coronavirus disease, also known as CCoV, is a very contagious intestinal infection that mostly affects puppies (Desai et al, 2020 a ) [7] . Human and animal gastrointestinal pathogens include group A rotaviruses (Tumlam et [35,19,20] . For collecting epidemiological information and determining the origin of unusual rotavirus strains, sequence analysis of the genes encoding the two outer capsid proteins VP7 and VP4, the inner capsid protein VP6, and the nonstructural protein NSP4 is helpful (Makwana et al, 2020 a ; Makwana et al, 2020 b ) [19][20] .…”

“…Human and animal gastrointestinal pathogens include group A rotaviruses (Tumlam et [35,19,20] . For collecting epidemiological information and determining the origin of unusual rotavirus strains, sequence analysis of the genes encoding the two outer capsid proteins VP7 and VP4, the inner capsid protein VP6, and the nonstructural protein NSP4 is helpful (Makwana et al, 2020 a ; Makwana et al, 2020 b ) [19][20] . There is a high probabilities of disease occurrence when animal transported from one place to other, movement of animals, or confine them together at one place as organized farm (Sakhare et al, 2019;Sharma et al, 2019) [31,33] .…”