First Report of Leaf Spot Disease Caused by <i>Exserohilum rostratum</i> on Bottle Gourd in India

Choudhary, Manoj; Sardana, H. R.; Bhat, M. Narayana; Gurjar, Malkhan Singh

doi:10.1094/pdis-02-18-0315-pdn

Cited by 8 publications

(1 citation statement)

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“…In reports from India, Choudhary et al, showed that E. rostratum was responsible for leaf spot disease of Lagenaria siceraria (a medicinal and nutritious domesticated cucurbit vegetable named as Bottle gourd) [47]. More recently E. rostratum was recorded as a cause of Petal Spot of Phalaenopsis Orchid in Taiwan [48].…”

Section: Effect Of Temperaturementioning

confidence: 99%

A First Record of Exserohilum rostratum as a New Pathogen Causing Bean Blight in Egypt.

Mf¹,

Attia²

2020

J Plant Pathol Microbiol

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Seedling blight of bean (Phaseolus vulgaris L.) was recorded in bean fields at five different localities in Beni Sweif Governorate, Egypt. Symptoms appeared as green dark to purplish-brown spots, with brown margins. The affected plant leaves were collected for mycological analysis. Percentage of disease incidence were 30%, 25%, 22%, 15% and 35% in El-Wasta, Nasser, Beni Sweif, Sumosta and Beba counties respectively. Leaf samples were surface sterilized and cultured on potato dextrose agar. The growing fungi were identified on morphological as well as on molecular basis. Microscopic examination revealed that the isolated organisms have the same characteristics of Exserohilum rostratum (Drechsler) Leonard & Suggs. Among the 30 fungal isolates collected from the five bean plantations, a representative isolate was grown for DNA extraction, PCR and rDNA sequencing. Universal primers targeting ITS regions of the rDNA were used for PCR and sequencing. Results confirmed that the sequences of these fungi showed close relationship with E. rostratum with 99.6% -100% similarity. The obtained sequences were deposited in the GenBank with accession numbers MT075801, MT071830, MT071831, MT071832, and MT071834. Pathogenicity tests confirmed that E. rostratum strains were pathogenic showing the same disease symptoms previously observed on bean plants in the study areas. The minimum temperature for spore germination was 5 °C, the optimum temperature was 35 °C, and the maximum temperature was 50 °C. On the other hand, conidial germination was stopped at both lower (3 °C) and higher (55 °C) temperatures. Studying the host range of the fungus showed that the pathogen was able to attack tomato, pepper, squash and potato beside common bean and watermelon.

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Section: Effect Of Temperaturementioning

confidence: 99%