First report of canine bufavirus in India

Abstract

Canine bufavirus (CBuV), a novel protoparvovirus of dogs that is associated with enteric and respiratory symptoms, has been reported only in Italy and China. The enteric prevalence of CBuV in India was investigated, and the nearly complete genome sequence (4292 bp) was amplified and reconstructed for one strain. A nucleotide sequence alignment indicated 93.42–98.81% identity to the other available CBuV sequences and 70.88–73.39% and 54.4–54.8% identity to human bufavirus and canine parvovirus 2 (CPV-2), respectively. The current strain is most closely related to Chinese CBuV strains, which together form an Asian lineage. This first report of the prevalence of CBuV in India emphasizes the need for further epidemiological surveillance. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-022-05398-7.

Investigation of canine chaphamaparvovirus, canine bufavirus, and canine adenovirus in dogs with diarrhea: First report of novel canine bufavirus in Turkey

“…We detected CBuV in two (3.22%) of 62 dogs. The low prevalence (1.74%–4.3%) of CBuV in the fecal samples of symptomatic dogs was consistent with previous reports in China and India [ 13 , 17 , 19 , 33 ]. These rates also match with that of human bufavirus (HuBuV, 0.3%–4.1%) [ 40 ].…”

“…According to our results, in one of CBuV and CaChPV positive dog, CPV-2b was also detected. While the striking finding in the other studies on CaChPV and CBuV prevalence was that the predominant virus in co-infections was CPV-2, here, we determined lower (1/48) [ 13 , 19 , 23 32 , 33 ]. Co-infections may cause severe gastroenteric signs in dogs [ 41 ].…”

“…The CBuV-2 can have predictable differences in antigenic and other biological properties [ 32 ]. We found that TR/27/CBuV/2021 had a 3-nucleotide (-CCA-) insertion at nucleotide position 427–429 of VP1, unlike other CBuVs [ 13 , 15 – 19 ]. Therefore, the VP1 protein of TR/27/CBuV/2021 contained 711 aa.…”

“…In China, the positivity rates of CBuV in Guangxi, Henan, and Anhui provinces ranged from 1.74% to 2.5% [ 13 , 16 , 17 , 33 ]. In a study in India, the positivity rate of CBuV was 4.3% [ 19 ]. Contrary to our results, some reports in Italy, Hungary, and China had a relatively higher prevalence of CBuV, ranging from 7.7% to 42.5% [ 15 , 16 , 32 ].…”

“…Accordingly, the virus was prevalent in dogs with respiratory signs and also a common component of the canine fecal virome [ 15 ]. To date, canine bufavirus (CBuV; species Carnivore protoparvovirus 3 , genus Protoparvovirus , and subfamily Parvovirinae ) was reported in Italy, Hungary, China, Canada, and India [ 13 , 15 – 19 ]. However, since the first discovery of CBuV in 2018, the virus has not yet been reported in Turkey.…”