First Documentation of In Vivo and In Vitro Ivermectin Resistance in Sarcoptes scabiei

Currie, Bart J.; Harumal, Pearly; McKinnon, Melita; Walton, Shelley F.

doi:10.1086/421776

Cited by 259 publications

(163 citation statements)

References 32 publications

(48 reference statements)

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“…Intensive use of ivermectin can lead to resistance, it has been documented for some intestinal helminthes (Soutello et al, 2007) . The resistance also noted against mites (Clark et al, 1995;Currie et al, 2004). From this point of view, it seems that ivermectin need synergistic product for rapid treatment of animals ecto-parasites.…”

Section: Introductionmentioning

confidence: 95%

Clinical Studies on the Efficacy of Different Topical Drugs with Ivermectin Injection in the Treatment of Egyptian Water Buffalo Calves Infested with Psoroptes Spp. Mites

Sobhy¹,

El-Damaty²,

El-Shazly³

2018

AJVS

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Key words:Psoroptes spp. mites, Ivermectin, Permethrin, Benzyl benzoate, Crotamiton A total of 30 water buffalo calves at smallholder farm suffering from skin lesions and clinical signs compatible with mange were investigated clinically and parasitologically. The efficacy of three topical drugs with ivermectin in the treatment of psoroptes mites was inspected. Infested calves were randomly allocated into four groups (6 calves each) in addition to positive untreated control group. The four un-control groups were administered ivermectin 1mL/50kg body weight by a single subcutaneous injection. Three groups were treated with different topical agents including benzyl benzoate 25%, crotamiton 10% and permethrin 5% once daily for two consecutive days. The number of live mites in skin scrapings was determined prior to treatment and at weekly intervals for eight weeks post treatment. Severity of skin lesions was evaluated and scored. The synergistic effect of each topical agent with ivermectin were compared for eliminate mites, aiming to obtain the ideal treatment model. Mite counts of the treated animals with Ivermectin plus permethrin were significantly (p < 0.05) lower than those of the Ivermectin plus benzyl benzoate at 2 weeks post-treatment. The efficiency of ivermectin plus permethrin was found to be significantly more effective in the treatment of mange even in the first days after application compared with other groups. Permethrin is a good and easily applied topical drug for treatment of psoroptes mites infestation in buffalo calves when used with ivermectin injection.

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Section: Introductionmentioning

confidence: 95%

Clinical Studies on the Efficacy of Different Topical Drugs with Ivermectin Injection in the Treatment of Egyptian Water Buffalo Calves Infested with Psoroptes Spp. Mites

Sobhy¹,

El-Damaty²,

El-Shazly³

2018

AJVS

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“…It has been reported that Sarcoptes scabiei has developed at least partial resistance to IV, 19 and IV has previously been used for the mass treatment of onchocerciasis in Senegal. 20 In addition, some authors have suggested low effectiveness in children, who constituted the majority of our sample, because excretion of IV is reduced in subjects with a low physiological rate of sebum production.…”

Section: Introductionmentioning

confidence: 99%

Ivermectin versus benzyl benzoate applied once or twice to treat human scabies in Dakar, Senegal: a randomized controlled trial

Caumes

Ndaw

et al. 2009

Bull World Health Org

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Objective To compare the effectiveness of oral ivermectin (IV) and two different modalities of topical benzyl benzoate (BB) for treating scabies in a community setting. Methods The trial included patients aged 5-65 years with scabies who attended the dermatology department at the Institut d'Hygiène Sociale in Dakar, Senegal. The randomized, open trial considered three treatments: a single application of 12.5% BB over 24 hours (BB1 group), two applications of BB, each over 24 hours (BB2 group), and oral IV, 150-200 µg/kg (IV group). The primary endpoint was the disappearance of skin lesions and itching at day 14. If necessary, treatment was repeated and patients were evaluated until cured. Results were analysed on an intention-to-treat basis. A pre-planned intermediate analysis was carried out after the BB1, BB2 and IV groups had recruited 68, 48 and 65 patients, respectively. Findings At day 14, 33 patients (68.8%) in the BB2 group were cured versus 37 (54.4%) in the BB1 group and 16 (24.6%) in the IV group (P < 10 -6 ). Bacterial superinfection occurred more often in the IV group than in the BB1 and BB2 groups combined (28% versus 7.8%, respectively; P = 0.006). At day 28, 46 patients (95.8%) in the BB2 group were cured versus 52 (76.5%) in the BB1 group and 28 (43.1%) in the IV group (P < 10 -5 ). These clear findings prompted early study cessation. Conclusion Topical BB was clearly more effective than oral IV for treating scabies in a Senegalese community.Une traduction en français de ce résumé figure à la fin de l'article. Al final del artículo se facilita una traducción al español. ‫املقالة.‬ ‫لهذه‬ ‫الكامل‬ ‫النص‬ ‫نهاية‬ ‫يف‬ ‫الخالصة‬ ‫لهذه‬ ‫العربية‬ ‫الرتجمة‬

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“…These secondary infections cause significant sequelae (cellulitis, septicemia, and post-streptococcal glomerulonephritis), and the increased community streptococcal burden contributes to extreme levels of acute rheumatic fever and rheumatic heart disease (4). Recently, it has been reported that treatment efficacy for scabies is decreasing (5,6), indicating that the development of novel control strategies is necessary.…”

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confidence: 99%

Characterization of a Serine Protease Homologous to House Dust Mite Group 3 Allergens from the Scabies Mite Sarcoptes scabiei

Beckham

Boyd

Reynolds

et al. 2009

Journal of Biological Chemistry

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The scabies mite, Sarcoptes scabiei var. hominis, infests human skin, causing allergic reactions and facilitating bacterial infection by Streptococcus sp., with serious consequences such as rheumatic fever and rheumatic heart disease. To identify a possible drug target or vaccine candidate protein, we searched for homologues of the group 3 allergen of house dust mites, which we subsequently identified in a cDNA library. The native protein, designated Sar s 3, was shown to be present in the mite gut and excreted in fecal pellets into mite burrows within the upper epidermis. The substrate specificity of proteolytically active recombinant rSar s 3 was elucidated by screening a bacteriophage library. A preference for substrates containing a RS(G/A) sequence at the P1-P2 positions was revealed. A series of peptides synthesized as internally quenched fluorescent substrates validated the phage display data and high performance liquid chromatography/mass spectrometry analysis of the preferred cleaved substrate and confirmed the predicted cleavage site. Searches of the human proteome using sequence data from the phage display allowed the in silico prediction of putative physiological substrates. Among these were numerous epidermal proteins, with filaggrin being a particularly likely candidate substrate. We showed that recombinant rSar s 3 cleaves human filaggrin in vitro and obtained immunohistological evidence that the filaggrin protein is ingested by the mite. This is the first report elucidating the substrate specificity of Sar s 3 and its potential role in scabies mite biology.

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First Documentation of In Vivo and In Vitro Ivermectin Resistance in Sarcoptes scabiei

Cited by 259 publications

References 32 publications

Clinical Studies on the Efficacy of Different Topical Drugs with Ivermectin Injection in the Treatment of Egyptian Water Buffalo Calves Infested with Psoroptes Spp. Mites

Clinical Studies on the Efficacy of Different Topical Drugs with Ivermectin Injection in the Treatment of Egyptian Water Buffalo Calves Infested with Psoroptes Spp. Mites

Ivermectin versus benzyl benzoate applied once or twice to treat human scabies in Dakar, Senegal: a randomized controlled trial

Characterization of a Serine Protease Homologous to House Dust Mite Group 3 Allergens from the Scabies Mite Sarcoptes scabiei

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