First detection and molecular identification of <i>Neospora caninum</i>
 from naturally infected cattle and sheep in North Africa

Amdouni, Yosra; Rjeibi, Mohamed Ridha; Awadi, Sofia; Rekik, Mourad; Gharbi, Mohamed

doi:10.1111/tbed.12828

Cited by 21 publications

(17 citation statements)

References 36 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our result provided molecular evidence that N. caninum infection is present in pigs in China, although the prevalence is significantly lower than that in cattle (22%), goats (19%) and sheep (10.6%) (Amdouni, Amairia, Said, Awadi, & Gharbi., ; Amdouni, Rjeibi, Awadi, Rekik, & Gharbi., ). Domestic pigs probably become infected with N. caninum mainly through ingestion of feedstuffs or drinking water contaminated by sporulated N. caninum oocysts (Dubey et al., ).…”

Section: Resultsmentioning

confidence: 97%

First report of Neospora caninum infection in pigs in China

Gui

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Neospora caninum is a protozoan parasite which can infect many mammals and birds with a worldwide distribution. However, no molecular data are available about the occurrence of N. caninum in pigs. In this study, the serological and molecular prevalence of N. caninum infection in farmed pigs were investigated in Hunan province, China, between January 2017 and December 2018. A total of 1,500 serum samples collected from 10 herds in Hunan province were evaluated using a competitive‐inhibition enzyme‐linked immunoassay assay (cELISA). The overall seroprevalence of N. caninum in the examined pigs was 1.9%. The seroprevalence of N. caninum ranged from 0.3% to 4.6% among different regions in Hunan province of China (p < .05). DNA was extracted from brain samples, and the Nc‐5 gene and ITS‐1 region were amplified and then sequenced. Three (0.5%) of the examined 600 brain tissues were found to contain N. caninum DNA. Our phylogenetic analyses indicated that N. caninum samples were classified into two distinct groups. Although the prevalence is low within the pig groups investigated, our results revealed the emergence of N. caninum infection in pigs in China. The finding of the present study provides molecular evidence that the pigs are the natural intermediate host of N. caninum and may have major epidemiological importance.

show abstract

Section: Resultsmentioning

confidence: 97%

First report of Neospora caninum infection in pigs in China

Gui

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…For neosporosis, the overall prevalence was 41.9%. However, in Africa, fewer studies have been conducted on sheep neosporosis even if molecular data have confirmed the infection in sheep in North Africa [27]. Kamga-Waladjo [10] found a prevalence of 0% and 62%, respectively, in sheep of the same production system and sheep from the extensive production system in Senegal.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Toxoplasma gondii and Neospora caninum infections in households sheep "Elevage en case" in Dakar, Senegal

Dahourou

Gbati²,

Savadogo

et al. 2019

Vet World

View full text Add to dashboard Cite

Aim: The study aimed to evaluate the occurrence of anti-Toxoplasma gondii and anti-Neospora caninum antibodies in sheep breeding in a particular husbandry system called "Elevage en case" in Dakar, Senegal. Materials and Methods: Blood samples were collected from 278 sheep. Serum was harvested and used for analysis. For the detection of T. gondii antibodies, 278 sera were analyzed using the modified agglutination test, while the enzyme linked-immunosorbent assay was used on 174 sheep sera to look for N. caninum antibodies. Results: This study showed that 29.4±6.8% of sheep carried both T. gondii and N. caninum antibodies. The overall prevalence was 60.1±5.7% and 41.9±7.3% for toxoplasmosis and neosporosis, respectively. For toxoplasmosis, the prevalence was higher in Gueule Tapée (63.3%) than in Medina (58.9%), but the variation was not significant (p=0.45). Regarding the age of animals, the prevalence was significantly higher in animals over 2 years old compared to those under 2 years old (p=0.002). For neosporosis, the prevalence was significantly higher in Medina (48.67%) than Gueule Tapée (16.7%) (p=0.001), but non-significant variation was noted according to animal age. Conclusion: The study showed that sheep reared in households have carried antibodies of T. gondii and N. caninum. The prevalence was high and it means that consumption of meat from these animals is risky if the meat is eaten undercooked.

show abstract

“…Serological-based methods and immunohistochemical staining are the most common techniques for detecting N. caninum infections in cattle and several hosts, as evidenced by an increasing amount of studies in countries from Africa (Amdouni et al, 2018), Asia (Pagmadulam et al, 2018;Yao et al, 2009;Yu et al, 2007) Nardoni et al, 2019;Rocchigiani et al, 2017). It has been used for the direct demonstration of parasite DNA in tissues and body fluids such as peripheral blood (Okeoma et al, 2004;Yao et al, 2009), serum (Bărburaș et al, 2019;McInnes et al, 2006), and semen (Amdouni et al, 2019;Ferre et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia

2021

View full text Add to dashboard Cite

Neospora caninum is a parasite of the phylum Apicomplexa that causes significant economic losses for cattle husbandry worldwide. Despite its relevance, information regarding infection prevalence in endemic areas of Colombia is scarce. Previous studies have reported a high seroprevalence in dairy cattle from Boyacá, which suggests a significant risk factor for active transmission of neosporosis. However, there is no available data concerning the infection stage or the presence of said parasite in the peripheral blood of dairy cattle. In this study, genetic detection of N. caninum was carried out using a nested PCR with an Nc-5 marker on peripheral blood samples from dairy cows in the municipalities of Paipa, Toca, and Tuta. Four positive samples were sequenced through the Sanger method, which were then edited, aligned, and compared to sequences available in the GenBank database. The parasite’s DNA was detected in 23 out of 170 analyzed blood samples. A qualitative detection limit was estimated (~64 parasites per volume of blood sampled). This is the first report of DNA detection of N. caninum through PCR in blood from Colombian dairy cattle. Thereupon, further studies about N. caninum molecular detection and population genetics in cattle peripheral blood could be very useful for the early diagnosis of neosporosis and creating more effective epidemiological surveillance strategies.

show abstract

First detection and molecular identification of Neospora caninum from naturally infected cattle and sheep in North Africa

Cited by 21 publications

References 36 publications

First report of Neospora caninum infection in pigs in China

First report of Neospora caninum infection in pigs in China

Prevalence of Toxoplasma gondii and Neospora caninum infections in households sheep "Elevage en case" in Dakar, Senegal

Genetic Detection of Neospora caninum in the Blood of Dairy Cattle from Boyacá, Colombia

Contact Info

Product

Resources

About