Neospora caninum is a parasite of the phylum Apicomplexa that causes significant economic losses for cattle husbandry worldwide. Despite its relevance, information regarding infection prevalence in endemic areas of Colombia is scarce. Previous studies have reported a high seroprevalence in dairy cattle from Boyacá, which suggests a significant risk factor for active transmission of neosporosis. However, there is no available data concerning the infection stage or the presence of said parasite in the peripheral blood of dairy cattle. In this study, genetic detection of N. caninum was carried out using a nested PCR with an Nc-5 marker on peripheral blood samples from dairy cows in the municipalities of Paipa, Toca, and Tuta. Four positive samples were sequenced through the Sanger method, which were then edited, aligned, and compared to sequences available in the GenBank database. The parasite’s DNA was detected in 23 out of 170 analyzed blood samples. A qualitative detection limit was estimated (~64 parasites per volume of blood sampled). This is the first report of DNA detection of N. caninum through PCR in blood from Colombian dairy cattle. Thereupon, further studies about N. caninum molecular detection and population genetics in cattle peripheral blood could be very useful for the early diagnosis of neosporosis and creating more effective epidemiological surveillance strategies.
Tomato cultivation ranks fourth in vegetable production in Boyaca. The largest production of Chonto tomato in greenhouses is supplied by the Alto Chicamocha irrigation and drainage district since it is the main agricultural production unit for the department. To investigate the physicochemical conditions of the soil and irrigation water in the crop and bacterial communities associated with the greenhouse Chonto tomato crop, a total of 15 crops of 50×50 m located within the irrigation and drainage district of Alto Chicamocha were analyzed. Soil and irrigation water samples were analyzed and the bacteriome associated with the crop rhizosphere was characterized. From the physicochemical results obtained, we observed a significant relationship between crop attributes, the physicochemical properties of soil, and irrigation water in the different study zones. On the other hand, in the bacterial composition associated with the crop (soil and irrigation water), a relationship was found between the genera Sphingomonas spp. and Sphingobium spp. with crop quality for the different crops evaluated.
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