First description of blaNDM-1, blaOXA-48, blaOXA-181 producing Enterobacteriaceae strains in Romania

Székely, Edit; Damjanova, Ivelina; Jánvári, Laura; Vas, Krisztina; Molnár, Szabolcs; Bilca, Doina; Lörinczi, Lilla; Tóth, Ákos

doi:10.1016/j.ijmm.2013.10.001

Cited by 49 publications

(44 citation statements)

References 24 publications

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“…In addition, specific PCRs for the detection of IncN2 (IncN2-for: 5=-GTCTAACGAGCTTACCGA AG-3=, IncN2-rev: 5=-GTTTCAACTCTGCCAAGTTC-3=) and IncHI1B (IncHI1B-for: 5=-GGAGCGATGGATTACTTCAGTA C-3=, IncHI1B-rev: 5=-TGCCGTTTCACCTCGTGAGTA-3=) that were previously described to be prevalent in bla NDM-1 -carrying plasmids also remained negative (14,19). As previously observed, the rmtC gene, encoding a 16S RNA methylase responsible for resistance to all aminoglycosides, was located on the bla NDM-1 -carrying plasmid (11). The IncL/M-type specific to the archetypal 61.8-kb self-conjugative plasmid harboring the bla OXA-48 gene was detected in all OXA-48 transconjugants by using primers previously described (20).…”

supporting

confidence: 66%

“…The OXA-48-producing K. pneumoniae isolates belonged to ST101 that had been prevalent among OXA-48-producing K. pneumoniae isolated in Europe (10), including Romania (11). The NDM-1-producing E. coli isolates belonged to the successful and virulent clone ST131, as previously observed with a strain that originated from India (12, 13), Thailand (14), and Târgu-Mureş, a town located in the central part of Romania (11). The NDM-1-producing E. cloacae isolates were of three different sequence types (STs) (ST93, ST171, ST255).…”

supporting

confidence: 65%

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Dissemination of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania

Dortet

Flonta²,

Boudehen

et al. 2015

Antimicrob Agents Chemother

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dFifteen carbapenemase-producing Enterobacteriaceae isolates and 12 carbapenemase-producing Pseudomonas aeruginosa isolates were recovered from patients hospitalized between August 2011 and March 2013 at the Hospital of Infectious Disease, ClujNapoca, Romania. One KPC-, nine NDM-1-, four OXA-48-, and one VIM-4-producing Enterobacteriaceae isolates along with 11 VIM-2-producing and one IMP-13-producing P. aeruginosa isolates were recovered from clinical samples. All carbapenemase genes were located on self-conjugative plasmids and were associated with other resistance determinants, including extendedspectrum ␤-lactamases and RmtC methylases.

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supporting

confidence: 66%

supporting

confidence: 65%

Dissemination of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania

Dortet

Flonta²,

Boudehen

et al. 2015

Antimicrob Agents Chemother

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“…Since the identification of OXA-181 in India in 2007, OXA-181-producing Enterobacteriaceae has been reported from several other countries in the Indian subcontinent, i.e., Bangladesh (9) and Sri Lanka (10) and potentially Nepal, as a Nepalese patient hospitalized in New Zealand was found to be carrying an OXA-181-producing K. pneumoniae (11). Outside the subcontinent, Enterobacteriaceae isolates producing OXA-181 have been found in Canada (12), France (13), the Netherlands (14), New Zealand (in a patient from Nepal) (11), Norway (in a patient from Romania) (15), Oman (16), Romania (17), Singapore (9), South Africa (18), and United Kingdom (in a patient from India) (19). In most cases (9, 11-14, 19, 20), the patients from whom OXA-181-producing Enterobacteriaceae were recovered had a recent travel history to the Indian subcontinent.…”

mentioning

confidence: 99%

“…The only exception was in a Citrobacter freundii strain; bla NDM-1 was not detected on the plasmid carrying bla OXA-181 , suggesting that bla NDM-1 is likely located on a different plasmid. It would be interesting to explore whether bla NDM-1 was located on an IncX3 plasmid within those strains carrying bla NDM-1 and bla OXA-181 (9,10,12,(15)(16)(17)29). On the other hand, as WCHEC14828 has no bla NDM-1 , it may also be possible that an IncX3 plasmid carrying bla OXA-181 has replaced the IncX3 plasmid carrying bla NDM-1 due to plasmid incompatibility.…”

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confidence: 99%

First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing

Liu

Feng

et al. 2015

Antimicrob Agents Chemother

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eWe report the first OXA-181-producing strain in China. bla OXA-181 was found in sequence type 410 (ST410) Escherichia coli strain WCHEC14828 from a Chinese patient without recent travel history. Genome sequencing and conjugation experiments were performed. bla OXA-181 was carried on a 51-kb self-transmissible IncX3 plasmid and was linked with qnrS1, a quinolone resistance gene. bla OXA-181 was introduced onto the IncX3 plasmid from a ColE2-type plasmid, and IncX3 plasmids have the potential to mediate the dissemination of bla OXA-181 .

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“…Previous epidemiological surveys in Romania have focused on the prevalence of Gram positive bacterial infections without molecular screening (Simon et al, 2010;Stoian et al, 2013;Pîrvănescu et al, 2014), which was performed often in Gram negative pathogens (Crăciunaş et al, 2010;Flonta et al, 2011, Székely et al, 2013.…”

Section: Introductionmentioning

confidence: 99%

Screening for phenotypic and genotypic resistance to antibiotics in Gram positive pathogens

Farkas¹,

Tarco²,

Crăciunaş³

et al. 2017

Studia UBB Biologia

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SUMMARY.Gram positive bacteria such as methicillin resistant Staphylococcus aureus, vancomycin resistant enterococci or multidrug resistant Streptococcus spp. are increasingly involved in severe infections with serious clinical consequences. The aim of this study is to investigate phenotypic and genotypic resistance traits in Gram positive pathogens isolated from clinical specimens in Cluj-Napoca, Romania. A total number of 31 Enterococcus spp., Staphylococcus spp. and Streptococcus spp. strains were subjected to antimicrobial susceptibility testing by disc diffusion, while the carriage of 26 antibiotic resistance genes and of class 1 integron was assessed by PCR. Bacterial pathogens included in this study were mostly susceptible to folate pathway inhibitors (100%), oxazolidinones (97%), fosfomycins (93%) and glycopeptides (92%). Enterococci, staphylococci and streptococci displayed high levels of phenotypic resistance to penicillins, tetracyclines and macrolides, a percentage of 42% being multidrug resistant. The strains under this study proved to be able to produce β-lactamase enzymes encoded by the TEM-1 gene and aminoglycoside modifying enzymes due to the carriage of aac(6')-Ie-aph(2") gene, to possess ribosomal protection mechanisms for macrolide and tetracycline resistance associated with ermB, ermC and tet(M) genes and to bear efflux genes tet(A), tet(B), tet(C) ant tet(L). Class 1 integron integrase was detected in 16% of the isolates, but no significant correlations were found between the carriage of intI1 gene and the phenotypic or genotypic resistance among the Gram positive pathogens investigated.

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First description of blaNDM-1, blaOXA-48, blaOXA-181 producing Enterobacteriaceae strains in Romania

Cited by 49 publications

References 24 publications

Dissemination of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania

Dissemination of Carbapenemase-Producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania

First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing

Screening for phenotypic and genotypic resistance to antibiotics in Gram positive pathogens

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