First Report of OXA-181-Producing Escherichia coli in China and Characterization of the Isolate Using Whole-Genome Sequencing

Abstract

eWe report the first OXA-181-producing strain in China. bla OXA-181 was found in sequence type 410 (ST410) Escherichia coli strain WCHEC14828 from a Chinese patient without recent travel history. Genome sequencing and conjugation experiments were performed. bla OXA-181 was carried on a 51-kb self-transmissible IncX3 plasmid and was linked with qnrS1, a quinolone resistance gene. bla OXA-181 was introduced onto the IncX3 plasmid from a ColE2-type plasmid, and IncX3 plasmids have the potential to mediate the dissemination of bla OXA-181 .

High Prevalence of Carbapenemase-Producing Enterobacteriaceae among Hospitalized Children in Luanda, Angola

“…Interestingly, the genetic environment of the bla OXA-181 gene was identical to that identified recently by Liu et al (20) in China, also on an IncX3-type plasmid, with the bla OXA-181 gene flanked upstream by ISEcp1 truncated by IS3000 and downstream by ISKpn19 followed by the qnrS1 gene. In other reports from different parts of the world, the bla OXA-181 gene was identified on different plasmid backbones, including IncN and IncT plasmids (19,21).…”

“…PCR mapping performed on all the positive isolates revealed that a remnant of the ISEcp1 element was located upstream of the bla OXA-181 gene, as previously reported on the IncX3-type plasmid pOXA-181 from a Chinese E. coli isolate (20), with ISEcp1 being truncated by the insertion of IS3000. Downstream of bla OXA-181 , and similar to what was observed on pOXA-181, ISKpn19 was identified.…”

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla NDM-7 in Enterobacteriaceae from Calgary, Canada

“…However, we were unable to establish such a link. pKpN01-NDM7 is an IncX3 plasmid; IncX3 plasmids are usually self-transferable and have been associated with the spread of several antimicrobial-resistant genes, including carbapenemase genes, such as bla NDM (33)(34)(35)(36)(37), bla KPC (38), and bla OXA-181 (39,40). A BLAST search against the GenBank sequence database revealed that pKpN01-NDM7 is highly similar to other bla NDMharboring IncX3 plasmids, including pNDM-HN380 (bla NDM-1 ) (36), p112298-NDM (bla NDM-1 ; GenBank accession number KP987216), pEc1929 (bla NDM-5 ; GenBank accession number KT824791), pNDM_MGR194 (bla NDM-5 ) (34), pJEG027 (bla NDM-4 ) (35), and pOM26-1 (bla NDM-7 ; GenBank accession number KP776609) (Fig.…”

Molecular Characterization of OXA-48-Like-Producing Enterobacteriaceae in the Czech Republic and Evidence for Horizontal Transfer of pOXA-48-Like Plasmids

“…1), encoding OXA-181, was an IncX3-type plasmid that was identical to pOXA181_EC14828 (100% coverage, 100% identity), which to date has been described only in China from an ST410, E. coli strain (WCHEC14828), isolated in 2014 (28). Similar to pOXA181_EC14828, the qnrS1 gene, conferring low-level resistance to fluoroquinolones, was identified in the sequence of pOXA-181_29144.…”