Fingerprint analysis of Ligusticum chuanxiong using hydrophilic interaction chromatography and reversed-phase liquid chromatography

Preparation of PDMAEMAHydrophilic Interaction Chromatographic Stationary Phase by Atom Transfer Radical Polymerization and Its Chromatographic Properties

“…但在此过程中, 各溶质的出峰顺序没有变 化, 说明流动相比例改变, 对溶质的选择性影响不大. 水-水溶性有机溶剂作为流动相时, 亲水性固定相表面 存在一层"富水层", 溶质的保留是通过在"富水层" 与流动相之间的分配作用实现的, 随着溶质的极性增 大, 保留增强 [16] . 可用公式(2)表示: …”

Quality Control Method for Herbal Medicine - Chemical Fingerprint Analysis

“…These methods have their own characteristics and scope of application, which has be discussed in the reference (Nie L et al, 2005). Generally, congruence coefficient and correlation coefficient (Jin Y. et al, 2008)was the workhorse of chemometrics in the similarity analysis of data sets. However, it has been aware that the (dis)similarities of the herbal objects have shortcomings (Liang, Y.-Z., et al, 2004;Christophe Tistaert et al, 2011): a reference fingerprint is necessary, but in many instances, the herbal medicines, selected for producing reference fingerprints, often do not have the best efficacy, which might influence the outcome.…”

“…A binary chromatographic fingerprint analysis was developed using hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) to gain more chemical information about two parts of water-extracted fraction: the polar compounds and weakly polar compounds. The fingerprint of the polar compounds was analyzed with HILIC, whereas the fingerprint of the weakly polar compounds was analyzed with RPLC (Jin Y et al, 2008). Except availability of more sensible detectors for HPLC, the appearance of capillary HPLC and ultra-performance (UPLC) have increased the analysis efficiency, so that shorter analytical time and better separation have been achieved (Zheng X.T.…”

Development of orthogonal two‐dimensional hydrophilic interaction chromatography systems with the introduction of novel stationary phases

“…The high organic modifier content gives HILIC two additional advantages: high sensitivity in ESI-MS, and faster separations due to the lower viscosity of HILIC eluents compared to standard RPLC eluents. In the past decades, HILIC has been widely used in the separation and detection of very polar compounds, such as sugar [3], pharmaceuticals [4,5], peptides [6,7], and some small polar compounds in bio-samples [8,9].The separation of very polar compounds by HILIC revealed much chemical information neglected in the common RPLC analysis [10]; however, the complexity of polar compounds gave a great challenge to their separation, which exceeds the capability of uni-dimensional chromatography. One of the most promising approaches to achieve this requirement is the development of 2D-LC [11 -14].…”