Fine mapping of qhir8 affecting in vivo haploid induction in maize

Liu, Chenxu; Li, Wei; Zhong, Yu; Dong, Xin; Hu, Haixiao; Tian, Xiaolong; Wang, Lele; Chen, Baojian; Chen, Chen; Melchinger, Albrecht E.; Chen, Shaojiang

doi:10.1007/s00122-015-2605-y

Cited by 53 publications

(55 citation statements)

References 26 publications

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“…In a first step, the PK6 NLD locus was introgressed over four generations into A188 to create a near‐isogenic inducer line (A188 NLD–PK6 ). The haploid induction rate of the successive backcrosses dropped from 3.59% in BC0S1 to 0.50% in BC3S1 (Table ), confirming the need of other favorable loci for enhanced haploid induction capacity (Prigge et al , ; Liu et al , ). This rather low induction rate remained significant because A188 was a non‐inducer line with an extremely low spontaneous haploid induction rate (0 haploids out of 4,487 seedlings tested, Table ).…”

Section: Resultsmentioning

confidence: 64%

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

et al. 2017

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Gynogenesis is an asexual mode of reproduction common to animals and plants, in which stimuli from the sperm cell trigger the development of the unfertilized egg cell into a haploid embryo. Fine mapping restricted a major maize QTL (quantitative trait locus) responsible for the aptitude of inducer lines to trigger gynogenesis to a zone containing a single gene () coding for a patatin-like phospholipase A. In all surveyed inducer lines, carries a 4-bp insertion leading to a predicted truncated protein. This frameshift mutation is responsible for haploid induction because complementation with wild-type abolishes the haploid induction capacity. Activity of the promoter is restricted to mature pollen and pollen tube. The translational NLD::citrine fusion protein likely localizes to the sperm cell plasma membrane. In roots, the truncated protein is no longer localized to the plasma membrane, contrary to the wild-type NLD protein. In conclusion, an intact pollen-specific phospholipase is required for successful sexual reproduction and its targeted disruption may allow establishing powerful haploid breeding tools in numerous crops.

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Section: Resultsmentioning

confidence: 64%

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

et al. 2017

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“…There have been various mapping and mechanism studies for haploid induction in maize (Dong et al, ; Li, Xu, Jin, & Chen, ; Liu et al, ; Prigge et al, ; Wu, Li et al, ; Wu, Ren et al, ; Zhao, Xu, Xie, & Jin, ), but only few investigated SHGD. Wu, Li et al () and Wu, Ren et al () reported a particular type of DHs, named “early DHs”, which were directly generated by in vivo haploid induction.…”

Section: Discussionmentioning

confidence: 99%

Genetic dissection of haploid male fertility in maize (Zea mays L.)

Yang

Chen

et al. 2019

Plant Breeding

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Haploid genome doubling is a key limiting step of haploid breeding in maize. Spontaneous restoration of haploid male fertility (HMF) provides a more promising method than the artificial doubling process. To reveal the genetic basis of HMF, haploids were obtained from the offspring of 285 F2:3 families, derived from the cross Zheng58 × K22. The F2:3 families were used as the female donor and Yu high inducer No. 1 (YHI‐1) as the male inducer line. The rates of HMF from each family line were evaluated at two field sites over two planting seasons. HMF displayed incomplete dominance. Transgressive segregation of haploids from F2:3 families was observed relative to haploids derived from the two parents of the mapping population. A total of nine quantitative trait loci (QTL) were detected, which were distributed on chromosomes 1, 3, 4, 7 and 8. Three major QTL, qHMF3b, qHMF7a and qHMF7b were detected in both locations, respectively. These QTL could be useful to predict the ability of spontaneous haploid genome doubling, and to accelerate the haploid breeding process by introgression or aggregation of those QTL.

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“…To delineate the map position and to identify closely linked markers more useful for marker-assisted selection in development of new inducers, qhir1 was fine-mapped to a 243 kb region (Dong et al 2013) and qhir8 to a 789 kb region (Liu et al 2015). Considering the confirmation of qhir1 in multiple studies, qhir1 may be considered mandatory for HI ability (Prigge et al 2012b), while other loci like qhir8 may enhance the function of qhir1 to increase the HIR (Liu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Considering the confirmation of qhir1 in multiple studies, qhir1 may be considered mandatory for HI ability (Prigge et al 2012b), while other loci like qhir8 may enhance the function of qhir1 to increase the HIR (Liu et al 2015). …”

Section: Introductionmentioning

confidence: 99%

Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

et al. 2017

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Key messageAmong the qhir11 and qhir12 sub-regions of a major QTL qhir1, only qhir11 has significant effect on maternal haploid induction, segregation distortion and kernel abortion.AbstractIn vivo haploid induction in maize can be triggered in high frequencies by pollination with special genetic stocks called haploid inducers. Several genetic studies with segregating populations from non-inducer x inducer crosses identified a major QTL, qhir1, on chromosome 1.04 contributing to in vivo haploid induction. A recent Genome Wide Association Study using 51 inducers and 1482 non-inducers also identified two sub-regions within the qhir1 QTL region, named qhir11 and qhir12; qhir12 was proposed to be mandatory for haploid induction because the haplotype of qhir11 was also present in some non-inducers and putative candidate genes coding for DNA and amino acid binding proteins were identified in the qhir12 region. To characterize the effects of each sub-region of qhir1 on haploid induction rate, F2 recombinants segregating for one of the sub-regions and fixed for the other were identified in a cross between CML269 (non-inducer) and a tropicalized haploid inducer TAIL8. To quantify the haploid induction effects of qhir11 and qhir12, selfed progenies of recombinants between these sub-regions were genotyped. F3 plants homozygous for qhir11 and/or qhir12 were identified, and crossed to a ligueless tester to determine their haploid induction rates. The study revealed that only the qhir11 sub-region has a significant effect on haploid induction ability, besides causing significant segregation distortion and kernel abortion, traits that are strongly associated with maternal haploid induction. The results presented in this study can guide fine mapping efforts of qhir1 and in developing new inducers efficiently using marker assisted selection.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-017-2873-9) contains supplementary material, which is available to authorized users.

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Fine mapping of qhir8 affecting in vivo haploid induction in maize

Cited by 53 publications

References 26 publications

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

Loss of pollen‐specific phospholipase NOT LIKE DAD triggers gynogenesis in maize

Genetic dissection of haploid male fertility in maize (Zea mays L.)

Dissection of a major QTL qhir1 conferring maternal haploid induction ability in maize

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