Fimbriated stationary phases for proteins

Várady, László; Mu, Ning; Yang, Yun; Cook, Steve; Afeyan, Noubar B.; Regnier, Fred E.

doi:10.1016/0021-9673(93)80510-f

Cited by 39 publications

(21 citation statements)

References 27 publications

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“…Polymeric modification procedures seem to be the prerequisite for making high capacity resins [49]. Later it will be shown that also alternative possibilities exist.…”

Section: The Hydrogel-effect ± the Reason For High Protein Capacity?mentioning

confidence: 99%

“…4. One additional benefit of the polymeric modification is the possibility to change the more hydrophobic character of base materials to a more hydrophilic form [49].…”

Section: Connecting Polymers To the Surface Of The Poresmentioning

confidence: 99%

“…According to the definition proposed by Varady et.al. [49] some of this materials should be regarded as ªfimbriatedº matrices, if long hydrophilic segments, located between two adsorbing units, extend in solution as a loop or a queue and take the high flexibility of polar filaments. A commercial available material with immobilized polyether is Toyopearl PPG 600M.…”

Section: Reviewmentioning

confidence: 99%

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Properties and Characterization of High Capacity Resins for Biochromatography

Müller¹

2005

Chem Eng & Technol

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At the moment ion exchanger resins display the highest binding capacities for proteins. Polymeric surface modification methods utilize the full pore space and allow to guarantee high capacities for small as well as for large proteins. Capacity maximization is also possible by pore size optimization fitting the pore size to the molecule size without loosing a lot of dynamic binding capacity under current process conditions. Further improvements of resins can be achieved by optimizing the pore structure and pore volume, which could enhance the mass transfer and so the dynamic binding capacity.

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“…Polymeric modification procedures seem to be the prerequisite for making high capacity resins [49]. Later it will be shown that also alternative possibilities exist.…”

Section: The Hydrogel-effect ± the Reason For High Protein Capacity?mentioning

confidence: 99%

“…4. One additional benefit of the polymeric modification is the possibility to change the more hydrophobic character of base materials to a more hydrophilic form [49].…”

Section: Connecting Polymers To the Surface Of The Poresmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

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Properties and Characterization of High Capacity Resins for Biochromatography

Müller¹

2005

Chem Eng & Technol

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“…Compared to the silica-based packings, the advantages of the synthesized WCX packings are (1) it can be used in a wider pH range and also has a higher column loading, as shown in Fig. 3, a comparable resolution [21]; (2) compared to dextran and agarose, it has better chemical and mechanical stabilities; and (3) compared to PS-DVS-based WCX packings, it has better resolution and a stronger hydrophilicity, resulting in a high mass recovery of proteins [13,24,25]. The WCX column was also used for the fast separation and purification of lysozyme from egg to obtain a satisfactory result.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of monodisperse poly(chloromethyl-styrene-co-divinylbenzene) beads and their application in separation of biopolymers

Gong

Zhu

et al. 2005

J. Sep. Science

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The monodisperse, porous poly(chloromethylstyrene-co-divinylbenzene) beads of 7.9 microm were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. Based on this media, a weak cation-exchange (WCX) stationary phase for HPLC was synthesized by a new chemically modified method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery, and good resolution for proteins. The dynamic protein-loading capacity of the synthesized WCX packings was 18.2 mg/g. Five proteins were separated in 3.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 5.0 min with only one step. The purity and specific bioactivity of the purified lysozyme were found to be more than 93% and 70 245 U/mg, respectively.

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“…For spherical solutes with radius r, in cylindrical pores of radius R p , K(r) is given by Eq. [4] (18):…”

Section: Size Exclusion Chromatographymentioning

confidence: 99%