Films based on bacteriorhodopsin in sol-gel matrices

Korposh, Sergiy; Sichka, M. Y.; Трикур, Іван Іванович; Sharkan, Y.P.; Yang, Do-Hyeon; Lee, SeungWoo; Ramsden, Jeremy J.

doi:10.1117/12.622631

Cited by 13 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Embedded into a gelatin matrix BR-containing PM fragments are durable (10 4 h) and resistant to solar light, the effects of oxygen, temperatures greater than 80 ºC (in water) and up to 140 ºC ( in air), pH = 112, and action of most proteases [32]. The dried PM are stacked on top of each other, focusing in the plane of the matrix, so that a layer with 1 m thickness contains about 200 monolayers [33]. When being illuminated such nanofilms exert the electric potential at 100200 mV, which coincides with the membrane potential of living cells [34].…”

Section: Resultsmentioning

confidence: 99%

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Mosin¹,

Ignatov²

2015

European Journal of Molecular Biotechnology

View full text Add to dashboard Cite

Halophiles (lat. "salt-loving") is the taxonomic group of extreme aerobic obligate Gramnegative microorganisms that live in conditions of high salinity -in the seas, salt lakes, saline soils etc. These microorganisms are known to reddish patina on products, preserved with using large quantities of salt (NaCl). Halophiles were isolated for the first time at the beginning of the XX century from the marine flora estuary mud, but their systematic study was started only at the end of the second decade of the XX century. The internal environment of the human body is not suitable for existence of halobacteria, since none of them are known to have pathogenic forms. Halobacteria have great practical potential for using in molecular bioelectronics and bionanotechnology due to their unique ability to convert the energy of sunlight into electrochemical energy of protons H + due to the presence in their cells a special photo transforming retinal containing integral protein -bacteriorhodopsin, the mechanism of action of which has been currently studied in detail. This article describes the characteristics of the metabolism and physiology of halophilic bacteria, as well as a method of biosynthesis and preparation of bacteriorhodopsin from purple membranes of cells of the extreme photoorganotrophic halobacterium Halobacterium halobium.

show abstract

Section: Resultsmentioning

confidence: 99%

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Mosin¹,

Ignatov²

2015

European Journal of Molecular Biotechnology

View full text Add to dashboard Cite

show abstract

“…Embedded in a gelatin matrix PM fragments are durable (10 4 h) and resistant to solar light, the effects of oxygen, temperatures greater than 80 0 C (in water) and up to 140 0 C (in air), pH = 112, and action of most proteases [24]. Dried PM are stacked on top of each other, focusing in the plane of the matrix, so that a layer with 1 m thickness contains about 200 monolayers [25]. When illuminated such nanofilms exert the electric potential 100200 mV, which coincides with the membrane potential of living cells [26].…”

Section: Resultsmentioning

confidence: 99%

Studying of Phototransformation of Light Signal by Photoreceptor Pigments - Rhodopsin, Iodopsin and Bacteriorhodopsin

Ignatov¹,

Mosin²

2014

Nanotechnology Research and Practice

View full text Add to dashboard Cite

This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano-and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color.Keywords: vision; rhodopsin; iodopsin; bacteriorhodopsin; additive color mixing. IntroductionVision (visual perception) is a process of psycho-physiological processing of the images of surrounding objects, carried out by the visual system, which allows to get an idea of the size, shape and color of surrounding objects, their relative position and distance between them. By means of this animals can receive 90 % of all incoming information to the brain.The function of the visual system is carried out through various interrelated complex structures designated as visual analyzer, consisting of a peripheral part (retina, optic nerve, optic tract) and the central department of combining stem and subcortical centers of the midbrain, as

show abstract

“…Як видно з отриманих даних, в результаті механічного шліфування та полірування кварцових оптичних волокон шорсткість поверхні відполірованого торця не перевищує 10 нм, що порівняно з шорсткістю поверхні покривних стекол, які використовуються як підкладки для нанесення плівок [3], та шорсткістю поверхні чистих желатинових або золь-гельних плівок, які використовуються в якості матриці [4]. Враховуючи отримані результати, можна зробити висновок про цілковиту придатність запропонованої технології механічної обробки поверхні торців волокон для їх підготовки до нанесення плівок бактеріородопсину.…”

Section: вступunclassified

“…При зростанні товщини Анологічно до плівок у желатинових матрицях наносилися плівки з використанням в якості матриці неорганічного SiO2-золь-гельного скла. Методики підготовки плівкоутворюючої суміші та отримання плівок описані в [2,4].…”

Section: вступunclassified