“…Dzul-Puc et al (2005), in a study with P. chrysosporium in sugarcane husk and pine seraglio estimated the influence of physiological age and substrate on benzo(α)pyrene degradation, concluding that the removal of the contaminant was most efficient when the inoculum age was 5-days, which was similar for L. crinitus in this work. Some authors have suggested against the use of a too young inoculum in field applications, as the colonization of the contaminated soil would be much slower and hinder the colonization because of the little mycelium biomass present in inoculum (Schmidt et al 2005;Walter et al 2005). Similar results were found in this study, which showed 10-days inoculum as the best.…”