Abstract-Biological processes have been proven to be efficient in treating landfill leachates. In this study, treatment of landfill leachate by Ecomat-immobilized mycelia of Ganoderma australe packed in a column was investigated. Continuous recycling of leachate at a constant flow (20 ml/min) was operated for 10 cycles to facilitate biological reactions. Diluted leachate (50%) and raw leachate (100%) were tested for comparison of efficiency of treatment. The results showed that biological oxygen demand (BOD 5 ) was not removed for diluted leachate and a slight removal of 0.14 and 1.72% for raw leachate after cycles 4 and 10, respectively. Chemical oxygen demand (COD) removal occurred after each cycle with diluted leachate demonstrating higher removal compared to raw leachate. The highest percentage of COD removal of 51.62% for diluted leachate and 22.79% for raw leachate were achieved after the tenth cycle. Ecomat-immobilized mycelium of Ganoderma australe could also reduce ammoniacal nitrogen (NH 3 -N) exhibiting highest reduction of 45.95% and 30.90% after cycle 8 for diluted and raw leachate, respectively. These findings suggested that a white rot fungus, G. australe has the ability to be considered as potential candidate in landfill leachate treatment.Index Terms-Fungal enzymes, ammoniacal nitrogen, chemical oxygen demand, biological treatment, white-rot fungi.
Landfill leachate is a complex environmental problem. Here, we used immobilized Ganoderma australe and crude enzyme to treat landfill leachate. The treatment by immobilized G. australe achieved 50% and 32% removals for leachate BOD5 and COD, respectively. Totals of 58%, 57%, and 62% of BOD, COD, and NH3-N percentage removal were obtained when the leachate previously treated with immobilized G. australe was continuously treated with crude enzymes. Using a continuous treatment with crude enzymes achieves higher percentages of COD and NH3-N removal.
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