Fibronectin coating of an experimental PTFE vascular prosthesis

Ramalanjaona, Georges; Kempczinski, Richard F.; Ogle, James D.; Silberstein, Edward B.

doi:10.1016/0022-4804(86)90165-4

Cited by 23 publications

(13 citation statements)

References 10 publications

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“…2). This was in accord with the findings of Ramalanjaona et al (9). However, the first-order curves of fibronectin binding in relation to incubation time observed by them were not seen in this study.…”

Section: Discussionsupporting

confidence: 93%

“…Fibronectin coated on ePTFE at a concentration of 50 kg/ml resisted shear stress of flow satisfactorily, retaining 70% after 120 min of exposure to flow. The loss in the first 30 rnin was 22%, compared to 69% observed by Ramalanjaona et al (9).…”

mentioning

confidence: 51%

“….0 x 10" molecules of fibronectin/cm* were bound to the graft ( Table 2), which was greater than that achieved using the perfusion pump (9).…”

mentioning

confidence: 78%

“…Manual precoating was employed in this study in contrast to the peristaltic pump used by Ramalanjaona et al (9). The number of molecules of fibronectin bound per squared centimeter of graft was estimated from the following factors: (a) molecular weight of fibronectin, 440,000 Da; (b) specific activity of fibronectin solution, 0.96 pCi/l pg; and (c) calibration factor of y counter, 1726 cpm/kCi.…”

Section: Discussionmentioning

confidence: 99%

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Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

et al. 1990

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Although fibronectin's role as a matrix to improve endothelial seeding has been demonstrated by other workers, the optimum concentration for use has never been described. Attachment of fibronectin to expanded polytetrafluoroethylene (ePTFE) was measured, using 125I‐radiolabeled protein, at different concentrations and for different time periods. The absolute amount of fibronectin bound to the graft increased with the concentrations used in coating (p < 0.001) and also with time (p < 0.01); e.g., at 50 μg/ml, 90 min of incubation produced a molecular attachment of 4.0 × 1011/cm2 of graft. However, its percentage attachment decreased with a rise in concentration (p < 0.001). After an initial loss of 22% in 30 min, the fibronectin‐graft bond was found to be stable when exposed to a shear stress produced by flow at 200 ml/min. No significant difference in the cell adherence could be found in grafts coated with fibronectin concentrations of 50, 150, and 250 μg/ml, although it was significantly less at 10 and 25 μg/ml (p < 0.05).

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 51%

“….0 x 10" molecules of fibronectin/cm* were bound to the graft ( Table 2), which was greater than that achieved using the perfusion pump (9).…”

mentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

et al. 1990

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“…13,14 They developed various coating methods to enhance the strength of endothelial cell attachment to the graft. Seeger and Klingman incubated and coated the luminal surface of the graft with fibronectin dissolved in a culture medium, 13 while Ramalanjaona et al diluted fibronectin with a physiologic solution and then perfused it into the interstices of the graft using a nonionic surfactant.…”

Section: Discussionmentioning

confidence: 99%

Enhanced graft healing of high-porosity expanded polytetrafluoroethylene grafts by covalent bonding of fibronectin

et al. 2000

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The effect of covalent bonding of fibronectin on the patency and graft healing of high-porosity expanded polytetrafluoroethylene (ePTFE) grafts was evaluated. Bilateral carotid grafting was performed in ten mongrel dogs using high-porosity (60 microm) ePTFE grafts, 4 cm in length and 4 mm in internal diameter, that either had been pretreated by the covalent bonding of fibronectin (fibronectin grafts) or were untreated (control grafts). The grafts were harvested 4 to 6 weeks after surgery and subjected to macroscopic and light-microscopic observations. There was no significant difference in patency between the fibronectin grafts and the control grafts with rates of 80% and 70%, respectively. The thrombus-free area score was significantly greater in the fibronectin grafts than in the control grafts, at 86.9% vs 34.0%. Furthermore, the pseudointima was better replaced by fibrous tissue in the fibronectin grafts than in the control grafts, being lined with a layer of endothelial-like cells. More transmural tissue ingrowth was evident in the fibronectin grafts than in the control grafts. The covalent bonding of fibronectin improves graft healing by stimulating transmural tissue ingrowth in high-porosity ePTFE grafts.

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Attachment of indium-111 labelled endothelial cells to pretreated polytetrafluoroethylene vascular grafts

Budd

Bell

James

1989

British Journal of Surgery

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This study investigated the effect of different surface coatings on endothelial cell attachment to polytetrafluoroethylene (PTFE) vascular grafts. Small segments of PTFE vascular grafts were precoated with one of a number of substances: gelatin, poly-l-lysin, fibronectin, or collagen type I, III or IV. Indium-111 labelled endothelial cells were then seeded on to the grafts and left for either 10, 30, 60 or 120 min. The unattached cells were removed and the degree of cell attachment was calculated. All coatings were significantly better at enhancing endothelial cell attachment at all times compared with controls, and fibronectin was significantly better as a coating material than any other material used. By pretreating with a substance such as fibronectin, the number of endothelial cells attaching to PTFE vascular grafts can be greatly increased, thereby enhancing the cell seeding process.

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Fibronectin coating of an experimental PTFE vascular prosthesis

Cited by 23 publications

References 10 publications

Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

Enhanced graft healing of high-porosity expanded polytetrafluoroethylene grafts by covalent bonding of fibronectin

Attachment of indium-111 labelled endothelial cells to pretreated polytetrafluoroethylene vascular grafts

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