Attachment of indium-111 labelled endothelial cells to pretreated polytetrafluoroethylene vascular grafts

Abstract

This study investigated the effect of different surface coatings on endothelial cell attachment to polytetrafluoroethylene (PTFE) vascular grafts. Small segments of PTFE vascular grafts were precoated with one of a number of substances: gelatin, poly-l-lysin, fibronectin, or collagen type I, III or IV. Indium-111 labelled endothelial cells were then seeded on to the grafts and left for either 10, 30, 60 or 120 min. The unattached cells were removed and the degree of cell attachment was calculated. All coatings were significantly better at enhancing endothelial cell attachment at all times compared with controls, and fibronectin was significantly better as a coating material than any other material used. By pretreating with a substance such as fibronectin, the number of endothelial cells attaching to PTFE vascular grafts can be greatly increased, thereby enhancing the cell seeding process.

Cellular engineering of vascular bypass grafts: Role of chemical coatings for enhancing endothelial cell attachment

“…Collagen: Tissue engineering with collagen shows up to three times more cellular attachment than native non-coated grafts (BUDD et al, 1989;HAEGERSTRAND et al, 1993;THOMSON et al, 1989;VOHRA et al, 1991). This result was not confirmed by others (SANK et al, 1992;STANSBY et al, 1994).…”

“…in comparative studies, FN shows enhanced cell attachment in comparison with collagen (BUDDet al, 1989;THOMSON et al, 1989), gelatin (BUDDet aL, 1989;IVARSSON et al, 1989;THOMSON et aL, 1989), poly-l-lysin (BUDD et al, 1989), laminin, albumin and gelatin (HASSON et aL, 1986;PRONK et al, 1994;THOMSON et al, 1989).…”

In vitro Attachment of Endothelial Cells to Different Graft Materials

“…It is known that an untreated ePTFE sur face has a low attachment of cells [7,8], Coat ing with laminin or fibronectin has altered this [7,8,11,12], but a preclotting process also enhances attachment [7,9] and is simple to accomplish. Fibronectin has the disadvan tage of being a favorite protein for staphylo coccus adherence [13,14], In spite of the pre clotting procedure, the attachment is much lower than that of preclotted Dacron.…”

Coating PTFE vascular prostheses with a fibroblastic matrix improves cell retention when subjected to blood flow

“…The vein repeatedly was flushed with Minimal Essential Medium (MEM, GIBCO BRL, Izasa, Madrid) under sterile conditions to remove red blood cells. It then was filled to distention with a 0.1% CLS collagenase type I solution in 15 mmol/L CaCl 2 (Lorne Laboratories Ltd., Twyford, U.K.) following the technique described by Budd et al 14 The resulting cell suspension was centrifuged at 200 g for 7 min and the supernatant discarded. The cell pellet was resuspended in complete M-199 medium consisting of standard M-199 (GIBCO BRL, Izasa, Madrid), 20% fetal bovine serum (GIBCO BRL, Izasa, Madrid), 90 g/mL heparin-porcine intestinal mucosa (SIGMA, Madrid), 2 mmol L-glutamine (GIBCO BRL, Izasa, Madrid), 20 g/mL endothelial growth factor (SIGMA, Madrid, Spain), 20 nmol Hepes buffer (GIBCO BRL, Izasa, Madrid), and 10,000 IU/ mL penicillin plus 10,000 g/mL streptomycin (GIBCO BRL, Izasa, Madrid).…”