Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

Vohra, Rajiv; Thompson, Gjl; Sharma, Hemant; Carr, H.M.H.; Walker, Michael G.

doi:10.1111/j.1525-1594.1990.tb01590.x

Cited by 36 publications

(27 citation statements)

References 12 publications

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“…At a concentration of 250 pglml at 90 min, 36 x 10" molecules of fibronectin/cm' were bound to the graft. This was three times greater than that reported by us for ePTFE (10) and may be attributed to the greater surface area of the knitted Dacron available for binding as well as to the presence of gelatin.…”

Section: Discussioncontrasting

confidence: 61%

“…Dissociation kinetics revealed early losses in the first 30 min, the bond between fibronectin and Gelseal being stable thereafter for up to 2 h. Fibronectin retention on Gelseal after 2 h of flow was only 22% compared to 70% with ePTFE (10). Retention of fibronectin to Gelseal was expected to be better than for ePTFE owing to the presence of binding sites on fibronectin for gelatin.…”

Section: Discussionmentioning

confidence: 97%

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Fibronectin Binding to Gelatin‐Impregnated Dacron (Gelseal) Prostheses

et al. 1992

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Endothelial cell adherence to uncoated gelatin-impregnated Dacron (Gelseal) is poor but can be significantly improved by precoating with a suitable basement membrane such as fibronectin. To assess the suitability of fibronectin-coated Gelseal for endothelial cell seeding, fibronectin binding to Gelseal and its dissociation kinetics were investigated. Fibronectin binding was quantified by radiolabeling human fibronectin with iodine 125, concentrations of 10, 25, SO, 150, and 250 pgiml being used to coat Gelseal at 30, 60, and 90 min of incubation. The amount of fibronectin bound was directly proportional ~ ~ ~~~ to the concentration used and increased with time of incubation ( p < 0.05). However, the percentage attachment decreased with increasing concentration (p < 0.001). The number of molecules bound per centimeter squared of graft was calculated. In the first 30 min, 75% of bciund fibronectin was lost after exposure to a flow rate of 200 mlimin in a pulsatile artificial circulation; thereafter, the fibronectin-Gelseal bond was stable for up to 2 h.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 97%

Fibronectin Binding to Gelatin‐Impregnated Dacron (Gelseal) Prostheses

et al. 1992

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“…To that end, we investigated the presence of three potential adhesion enhancing proteins, fibronectin, laminin, and type I collagen. Fibronectin has been shown to enhance EC retention under flow in numerous studies; 2,27,29,42,50,57,66 however, EC retentions were much less than 100%. Laminin is a major component of the basal lamina 1 and has been used with very limited success as a graft coating.…”

Section: Discussionmentioning

confidence: 98%

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

2006

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It is generally accepted that endothelialization and subsequent development of a functional endothelium are of paramount importance to the success of any bioartificial artery. In this study, we aimed to assess the ability of smooth muscle cell-remodeled, fibrin-based media-equivalents (MEs) to be endothelialized, examine the morphological changes of endothelial cells (ECs) associated with exposure to physiologically-relevant shear stress in a custom-built bioreactor, and determine if adherent ECs are capable of withstanding average physiological shear stresses. It was found that MEs could be readily endothelialized with surface coverages of 98.8 +/- 0.9% after two days, and the ECs expressed von Willebrand factor. Furthermore, EC retention remained high (steady: 96.5 +/- 4.4%, pulsatile: 94.3 +/- 4.3%) under exposure to physiologically relevant shear stresses for 48 h. The results indicate that these MEs are conducive to generating an EC monolayer, with the ECs possessing adhesion strength sufficient to withstand physiological shear stress and maintain a normal phenotype.

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“…For example, Rotmans and colleagues successfully captured endothelial progenitor cells on ePTFE grafts by immobilizing anti‐CD34 antibodies to the surface . Other groups have used laminin, extracellular matrix (ECM) protein, or cell‐adhesive peptide modification of ePTFE to improve endothelialization . While these strategies have shown some success in adhering and retaining ECs to an ePTFE graft surface, they do not directly address the second issue of IH, and in some cases even enhance intimal thickening …”

Section: Introductionmentioning

confidence: 99%

“…6 Other groups have used laminin, extracellular matrix (ECM) protein, or cell-adhesive peptide modification of ePTFE to improve endothelialization. [7][8][9][10][11] While these strategies have shown some success in adhering and retaining ECs to an ePTFE graft surface, they do not directly address the second issue of IH, and in some cases even enhance intimal thickening. 6,7 Intimal hyperplasia in ePTFE grafts develops over the course of a few weeks to 2 years, usually at the anastomotic sites.…”

Section: Introductionmentioning

confidence: 99%

Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts

Bastijanic

Kligman

Marchant

et al. 2015

J Biomedical Materials Res

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Expanded polytetrafluoroethylene (ePTFE) grafts were coated on the luminal surface with a cell-adhesive fluorosurfactant (FSP) polymer to promote endothelialization, followed by ethanol hydration to degas the pores and subsequent cell-adhesive, enzymatically degradable poly(ethylene glycol)-based hydrogel incorporation into the graft interstices to accommodate potential smooth muscle cell integration in the graft wall. The FSP coating on ePTFE was stable as demonstrated by a significantly reduced receding water contact angle on FSP-coated ePTFE (14.5 ± 6.4°) compared to uncoated ePTFE (105.3 ± 4.5°, P < 0.05) after ethanol exposure. X-ray photoelectron spectroscopy analysis of the same surfaces confirmed FSP presence. Localization of the FSP and hydrogel within the ePTFE graft construct was assessed using fluorescently labeled polymers, and demonstrated hydrogel infiltration throughout the thickness of the graft wall, with FSP coating limited to the lumen and adventitial surfaces. FSP at the luminal surface on dual-coated grafts was able to bind endothelial cells (EC) (98.7 ± 23.1 cells/mm(2) ) similar to fibronectin controls (129.4 ± 40.7 cells/mm(2) ), and significantly higher than uncoated ePTFE (31.6 ± 19 cells/mm(2,) P < 0.05). These results indicate that ePTFE grafts can be simultaneously modified with two different polymers that have potential to directly address both endothelialization and intimal hyperplasia. Such a construct is a promising candidate for an off-the-shelf synthetic graft for small-diameter graft applications.

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Fibronectin Coating of Expanded Polytetrafluoroethylene (ePTFE) Grafts and Its Role in Endothelial Seeding

Cited by 36 publications

References 12 publications

Fibronectin Binding to Gelatin‐Impregnated Dacron (Gelseal) Prostheses

Fibronectin Binding to Gelatin‐Impregnated Dacron (Gelseal) Prostheses

Endothelialization and Flow Conditioning of Fibrin-Based Media-Equivalents

Dual biofunctional polymer modifications to address endothelialization and smooth muscle cell integration of ePTFE vascular grafts

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