Fibrin Glue with Autogenic Bone Marrow Mesenchymal Stem Cells for Urethral Injury Repair in Rabbit Model

Wang, Kai; Guan, Yong; Liu, Yi; Zhu, Meifeng; Li, Ting; An, Di; Ou, Lailiang; Che, Yongzhe; Zhang, Guorong; Zhang, Jun; Zheng, Xi-Long

doi:10.1089/ten.tea.2011.0359

Cited by 24 publications

(11 citation statements)

References 31 publications

(26 reference statements)

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“…To date, many attempts have been made to determine appropriate substitutes for urethral reconstruction, and in general, it is believed that seeding cells is necessary for constructing an artificial biomaterial scaffold that is suitable for reconstruction of extensive urethral defects. Various types of cells have been adopted in animal and clinical experiments, such as bone marrow mesenchymal stem cells [19][20][21], urethral epithelial cells [22] and smooth muscle cells [6,7,[23][24][25], urine-derived stem cells [26], mesothelial cells [27], adipose-derived stem Cells [28,29], and oral keratinocytes [30], and other cell types.…”

Section: Discussionmentioning

confidence: 99%

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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Section: Discussionmentioning

confidence: 99%

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia

Tang

et al. 2015

Biomaterials

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“…Recently, several approaches have been employed to improve vascularization of tissue engineering scaffolds, including optimization of 3D structures [ 5 , 6 ]; preseeding of mesenchymal stem cells (MSCs) [ 7 ], endothelial cells (ECs) [ 2 ], and endothelial progenitor cells (EPCs) [ 8 ]; and the loading of bioactive molecules, such as NO donor [ 9 ], angiogenic factors [ 10 , 11 ], or peptides [ 12 ] to the scaffold. However, until now none of them have been proven fully successful in development of long-lasting vasculature (blood vessels).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Vascularization in Hybrid PCL/Gelatin Fibrous Scaffolds with Sustained Release of VEGF

Wang

Chen

Pan

et al. 2015

BioMed Research International

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Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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“…Hemostatic agents have been used to control hemorrhage during surgical procedures for harvesting bone grafts from donor sites. Collagen sponge and fibrin glue are employed as hemostatic agents and as scaffolds for bone and cartilage tissue engineering 2 , 16 , 19 , 23 , 29 . Additionally, collagen sponge acts as wound burn dressing material and as intravaginal contraceptive diaphragm 2 , 14 .…”

Section: Introductionmentioning

confidence: 99%

Effect of collagen sponge and fibrin glue on bone repair

et al. 2015

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The ability of hemostatic agents to promote bone repair has been investigated using in vitro and in vivo models but, up to now, the results are inconclusive. Objective In this context, the aim of this study was to compare the potential of bone repair of collagen sponge with fibrin glue in a rat calvarial defect model.Material and Methods Defects of 5 mm in diameter were created in rat calvariae and treated with either collagen sponge or fibrin glue; untreated defects were used as control. At 4 and 8 weeks, histological analysis and micro-CT-based histomorphometry were carried out and data were compared by two-way ANOVA followed by Student-Newman-Keuls test when appropriated (p≤0.05).Results Three-dimensional reconstructions showed increased bone formation in defects treated with either collagen sponge or fibrin glue compared with untreated defects, which was confirmed by the histological analysis. Morphometric parameters indicated the progression of bone formation from 4 to 8 weeks. Additionally, fibrin glue displayed slightly higher bone formation rate when compared with collagen sponge.Conclusion Our results have shown the benefits of using collagen sponge and fibrin glue to promote new bone formation in rat calvarial bone defects, the latter being discreetly more advantageous.

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Fibrin Glue with Autogenic Bone Marrow Mesenchymal Stem Cells for Urethral Injury Repair in Rabbit Model

Cited by 24 publications

References 31 publications

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Enhanced Vascularization in Hybrid PCL/Gelatin Fibrous Scaffolds with Sustained Release of VEGF

Effect of collagen sponge and fibrin glue on bone repair

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