Effect of collagen sponge and fibrin glue on bone repair

Santos, Thiago de Santana; Abuna, Rodrigo Paolo Flores; Almeida, Ana Carolina de; Beloti, Márcio Mateus; Rosa, Adalberto Luiz

doi:10.1590/1678-775720150374

Cited by 25 publications

(23 citation statements)

References 30 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The healing of calvarial bone defects usually begins by the margins of the surgical wound, from the periosteum or the dura mater . This minimal presence of neoformed bone tissue confirms the permanent character of the defect and is in agreement with the results of previous research, in which the filling of critical bone defects with collagen sponge alone resulted in minimal bone formation and persistence of the bone defect . Histological analyses also confirmed the data obtained through micro‐CT quantitative analysis, which showed that 5 mM Sr 2+ SR was inefficient in stimulating the regeneration of the bone defects.…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, this material is biodegradable and promotes cell migration due to the presence of (Arg‐Gly‐Asp) domains that bind to cell surface integrins . The collagen sponge adopted in this study, also used as a carrier by other research groups, exhibits expansion capacity in addition to allowing adequate soaking of the test solution. Consequently, the sponge adapts to the size of the defect, promotes the efficient tamponade of the lesion and favors the formation of a stable clot, being reabsorbed after four to six weeks .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Local delivery of strontium ranelate promotes regeneration of critical size bone defects filled with collagen sponge

Masalskas

Júnior

Leoni

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

The effect of local delivery of strontium ranelate (SR) on bone regeneration of critical size bone defects filled with collagen sponge was evaluated. Bone defects of 5 mm diameter created in rat calvaria were filled with collagen sponge (C); collagen sponge with 5 mM Sr SR (C5SR) or collagen sponge with 50 mM Sr SR (C50SR). After 2, 4, and 6 weeks, bone volume (BV), bone surface (BS), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were evaluated by computed microtomography. At 6 weeks, histological analysis was performed. Intragroup comparisons were made by the Friedman test, while comparisons between groups were made by Kruskal-Wallis test (α = 5%). All groups showed increased BV, BS, Tb.Th, and Tb.N over time, but only C50SR promoted the reduction of Tb.Sp (p < 0.05). No significant differences between groups were detected at weeks 2 and 4. However, C50SR showed the highest values of BV, BS, and Tb.Th at 6 weeks (p < 0.05). Histological analysis revealed connective tissue in C and C5SR and immature bone tissue in C50SR. Local delivery of SR 50 mM Sr associated with collagen sponge increased and accelerated bone regeneration in critical bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 333-341, 2018.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Local delivery of strontium ranelate promotes regeneration of critical size bone defects filled with collagen sponge

Masalskas

Júnior

Leoni

et al. 2017

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Santos Tde et al . () have proved that the collagen sponge can promote bone repair through the repair of calvarial defect on mice. However, due to the low porosity of stent and collagen sponge, it is hardly to provide enough space to ensure homogenous cells and extracellular matrix distribution (Ng et al .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…Till now, the collagen fibres have been widely used in the manufacture of biodegradable scaffolds and haemostatic sponges (Miyoshi et al 2001). Santos Tde et al (2015) have proved that the collagen sponge can promote bone repair through the repair of calvarial defect on mice. However, due to the low porosity of stent and collagen sponge, it is hardly to provide enough space to ensure homogenous cells and extracellular matrix distribution (Ng et al 2004).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

C-terminal domains of bacterial proteases: structure, function and the biotechnological applications

Huang

Liú

et al. 2016

J Appl Microbiol

View full text Add to dashboard Cite

Summary C‐terminal domains widely exist in the C‐terminal region of multidomain proteases. As a β‐sandwich domain in multidomain protease, the C‐terminal domain plays an important role in proteolysis including regulation of the secretory process, anchoring and swelling the substrate molecule, presenting as an inhibitor for the preprotease and adapting the protein structural flexibility and stability. In this review, the diversity, structural characteristics and biological function of C‐terminal protease domains are described. Furthermore, the application prospects of C‐terminal domains, including polycystic kidney disease, prepeptidase C‐terminal and collagen‐binding domain, in the area of medicine and biological artificial materials are also discussed.

show abstract

“…Site-directed mutagenesis showed that the conserved polar and aromatic residues in PPC domains might play key roles in the binding and swelling of collagen. Collagen fibers have been widely used to manufacture biodegradable scaffolds and hemostatic sponges; however, the low porosity of stents and collagen sponges has limited the distribution of the extracellular matrix and the growth of homogenous cells (40,41). The collagen-binding and swelling capacities of PPC can be used to modify collagen fibers and create novel stents or sponges with high porosity ratios and good physical properties, which might be useful for facilitating cell proliferation and promoting tissue repair.…”

mentioning

confidence: 99%

Mechanistic Insight into the Binding and Swelling Functions of Prepeptidase C-Terminal (PPC) Domains from Various Bacterial Proteases

Huang

Wü

Liú

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

The bacterial prepeptidase C-terminal (PPC) domain can be found in the C termini of a wide variety of proteases that are secreted by marine bacteria. However, the functions of these PPC domains remain unknown due to a lack of systematic research. Here, the binding and swelling abilities of eight PPC domains from six different proteases were compared systematically via scanning electron microscopy (SEM), enzyme assays, and fluorescence spectroscopy. These PPC domains all possess the ability to bind and swell insoluble collagen. PPC domains can expose collagen monomers but cannot disrupt the pyridinoline cross-links or unwind the collagen triple helix. This ability can play a synergistic role alongside collagenase in collagen hydrolysis. Site-directed mutagenesis of the PPC domain from Vibrio anguillarum showed that the conserved polar and aromatic residues Y6, D26, D28, Y30, W42, E53, C55, and Y65 and the hydrophobic residues V10, V18, and I57 played key roles in substrate binding. Molecular dynamic simulations were conducted to investigate the interactions between PPC domains and collagen. Most PPC domains have a similar mechanism for binding collagen, and the hydrophobic binding pocket of PPC domains may play an important role in collagen binding. This study sheds light on the substrate binding mechanisms of PPC domains and reveals a new function for the PPC domains of bacterial proteases in substrate degradation. IMPORTANCE Prepeptidase C-terminal (PPC) domains commonly exist in the C termini of marine bacterial proteases. Reports examining PPC have been limited, and its functions remain unclear. In this study, eight PPCs from six different bacteria were examined. Most of the PPCs possessed the ability to bind collagen, feathers, and chitin, and all PPCs could significantly swell insoluble collagen. PPCs can expose collagen monomers but cannot disrupt pyridinoline cross-links or unwind the collagen triple helix. This swelling ability may also play synergistic roles in collagen hydrolysis. Comparative structural analyses and the examination of PPC mutants revealed that the hydrophobic binding pockets of PPCs may play important roles in collagen binding. This study provides new insights into the functions and ecological significance of PPCs, and the molecular mechanism of the collagen binding of PPCs was clarified, which is beneficial for the protein engineering of highly active PPCs and collagenase in the pharmaceutical industry and of artificial biological materials.

show abstract

Effect of collagen sponge and fibrin glue on bone repair

Cited by 25 publications

References 30 publications

Local delivery of strontium ranelate promotes regeneration of critical size bone defects filled with collagen sponge

Local delivery of strontium ranelate promotes regeneration of critical size bone defects filled with collagen sponge

C-terminal domains of bacterial proteases: structure, function and the biotechnological applications

Mechanistic Insight into the Binding and Swelling Functions of Prepeptidase C-Terminal (PPC) Domains from Various Bacterial Proteases

Contact Info

Product

Resources

About