Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Jia, Wenyu; Tang, He; Wu, Jianjian; Hou, Xun; Chen, Bing; Chen, Wei; Zhao, Yannan; Shi, Chunying; Zhou, Feng; Yu, Wei; Huang, Shu‐Zhen; Ye, Gang; Dai, Jianwu

doi:10.1016/j.biomaterials.2015.08.009

Cited by 62 publications

(44 citation statements)

References 41 publications

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“…(151, [168][169][170] implantation of a collagen membrane functionalized with CBD-VEGF 1 or injection of this protein 2 in a rat infarcted myocardium  1 better neovascularization of the myocardium  1 reduction of the scar size  1 enhancement of the capillary growth  1 improvement of the cardiac functions  2 CBD-VEGF was almost undetectable in the serum 3 to 6 h post injection, in stark contrast with native VEGF121 CBD-VEGF121 [ TKKTLRT ] (171,172) full-thickness injury uterus 1 (rat), extensive urethral defect 2 (dog)  1 improvement of the pregnancy rate at the scar site (50 vs 19% for VEGF)  2 good regeneration of the urethra (  no VEGF control) VEGF164-CBD [ S3 domain ] (173)(174)(175) subcutaneous injection in a rat dorsal-skin flap model  decrease in the necrosis rate and promotion of the neoangiogenesis  controversial study (concerning the lack of bioactivity of native VEGF)…”

Section: Cbd-vegf121 [ Tkktlrt ]mentioning

confidence: 99%

Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration

Addi

Murschel

2017

Tissue Engineering Part B: Reviews

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Collagen-based biomaterials are widely used in the field of tissue engineering; they can be loaded with biomolecules such as growth factors (GFs) to modulate the biological response of the host and thus improve its potential for regeneration. Recombinant chimeric GFs fused to a collagen-binding domain (CBD) have been reported to improve their bioavailability and the host response, especially when combined with an appropriate collagen-based biomaterial. This review first provides an extensive description of the various CBDs that have been fused to proteins, with a focus on the need for accurate characterization of their interaction with collagen. The second part of the review highlights the benefits of various CBD/GF fusion proteins that have been designed for wound healing and bone regeneration.

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Section: Cbd-vegf121 [ Tkktlrt ]mentioning

confidence: 99%

Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration

Addi

Murschel

2017

Tissue Engineering Part B: Reviews

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“…Although, most CPO based oxygen releasing systems have used hydrophobic polymers, such as PDMS17 or polyurethane,40 we used collagen (i.e., a naturally occurring hydrophilic system), given its excellent biocompatibility and low immunogenicity 41. As collagen is a component of the ECM,9 islets that are seeded into our bioscaffold will therefore be exposed to a microenvironment similar to that of the native pancreas which, in turn, helps to maintain their function as well as regulate their cellular activities 42.…”

Section: Discussionmentioning

confidence: 99%

A Collagen Based Cryogel Bioscaffold that Generates Oxygen for Islet Transplantation

Razavi

Primavera

Kevadiya

et al. 2020

Adv Funct Materials

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The aim of this article is to develop, characterize, and test a novel 3D bioscaffold matrix that can accommodate pancreatic islets and provide them with a continuous, controlled, and steady source of oxygen to prevent hypoxia-induced damage following transplantation. Hence, a collagen-based cryogel bioscaffold that incorporates calcium peroxide (CPO) into its matrix is made. The optimal concentration of CPO integrated into bioscaffolds is 0.25 wt% and this generates oxygen at 0.21 ± 0.02 × 10 -3 m day -1 (day 1), 0.19 ± 0.01 × 10 -3 m day -1 (day 6), 0.13 ± 0.03 × 10 -3 m d -1 (day 14), and 0.14 ± 0.02 × 10 -3 m d -1 (day 21). Accordingly, islets seeded into cryogel-CPO bioscaffolds have a significantly higher viability and function compared to islets seeded into cryogel alone bioscaffolds; these findings are supported by data from quantitative computational modeling. When syngeneic islets are transplanted into the epididymal fat pad (EFP) of diabetic mice, the cryogel-0.25 wt%CPO bioscaffold improves islet function with diabetic animals re-establishing glycemic control. Mice transplanted with cryogel-0.25 wt%CPO bioscaffolds show faster responses to intraperitoneal glucose injections and have a higher level of insulin content in their EFP compared to those transplanted with islets alone (P < 0.05). The novel oxygen-generating bioscaffold (i.e., cryogel-0.25 wt%CPO) therefore provides a biostable and biocompatible 3D microenvironment for islets which can facilitate islet survival and function at extra-hepatic sites of transplantation.

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“…Jia et al reported that it is difficult to obtain enough cultured cells for clinical application, because primary cells generally require complicated processes of culture, expansion and induced differentiation in vitro after harvest. 28 They also mentioned the high cost of these processes. In addition, the cost of constructing a cellularized graft is generally sixfold higher than Severe injuries and strictures were shown in group 3.…”

Section: Discussionmentioning

confidence: 99%

Insulin‐like growth factor 1 sustained‐release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model

et al. 2019

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Objectives To evaluate the preventive effect of an insulin‐like growth factor 1 sustained‐release collagen urethral catheter on urethral stricture after urethral injury in a rabbit model. Methods We made urethral catheters coated either with insulin‐like growth factor 1 impregnated collagen or with only collagen, and we divided 19 male Japanese white rabbits into three groups according to the kind of catheter inserted immediately after the rabbit's urethra was injured by electrocoagulation. Group 1 (n = 7) had a catheter coated with insulin‐like growth factor 1 impregnated collagen inserted; group 2 (n = 7) had a catheter coated with only collagen inserted; and group 3 (n = 5) had an uncoated catheter inserted. A total of 14 days later, the injured urethras were evaluated by urethrography and urethroscopy, and were also histologically examined. Results Urethrography showed that the ratio of the urethral lumen diameter in injured urethra to that in normal urethra was the largest in group 1 (P < 0.0001). In addition, five of the seven rabbits in group 1 (71.4%) had a urethral lumen large enough for passage of a urethroscope, a fraction larger than the corresponding fractions in groups 2 (57.1%) and 3 (20%). On histological analysis, the injured area not covered with regenerated urethral epithelium tended to be smaller in group 1 than the other two groups, but the mean difference was not significant (P = 0.19). Conclusions An insulin‐like growth factor 1 sustained‐release collagen urethral catheter significantly improves wound healing and prevents urethral stricture after urethral injury.

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Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model

Cited by 62 publications

References 41 publications

Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration

Design and Use of Chimeric Proteins Containing a Collagen-Binding Domain for Wound Healing and Bone Regeneration

A Collagen Based Cryogel Bioscaffold that Generates Oxygen for Islet Transplantation

Insulin‐like growth factor 1 sustained‐release collagen on urethral catheter prevents stricture after urethral injury in a rabbit model

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