Fetal mesenchymal stem cells: isolation, properties and potential use in perinatology and regenerative medicine

Gucciardo, Léonardo; Lories, Rik; Ochsenbein‐Kölble, Nicole; Doné, Elisa; Zwijsen, An; Deprest, Jan

doi:10.1111/j.1471-0528.2008.02005.x

Cited by 89 publications

(76 citation statements)

References 59 publications

(65 reference statements)

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“…From amnion tissue both epithelial and stromal amnion cells can be obtained, representing an interesting cell types to be tested in horse for differentiation processes other than the mesenchymal lineage. Recently placenta-derived stem cells have attracted attention as a novel cell source for cell transplantation (Gucciardo et al 2009) as, among other features; the harvest of such cells does not require any invasive treatment. Interestingly, being fetal cells, amnion-derived cells could offer a number of therapeutic advantages over adult stem cells as they can differentiate in vitro into diverse cells types, have low immunogenicity and anti-inflammatory function, making them well suited for cell replacement therapy (Parolini et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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Stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. In the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. Amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. The expression of stem cell markers such as Oct-4, Nanog, Sox-2 and CD105 was assessed by retrotranscription quantitative PCR (RT-qPCR) and differentiation into adipocytes, osteocytes and chondrocytes precursors was analyzed by cytochemical staining. This study showed that amnion stromal cells expressing stem cell markers can differentiate into mesoderm lineage and may be an alternative source to mesenchymal stem cells derived from adipose tissue and bone marrow for the use in tissue repair.

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Section: Discussionmentioning

confidence: 99%

Isolation and differentiation potential of an equine amnion-derived stromal cell line

et al. 2011

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“…Adherent stromal cells with similar characteristics were subsequently isolated from other tissues such as adipose, dental pulp, muscle, liver and brain (Porada et al 2006;Huang et al 2009). They are also found in foetal and extraembryonic tissues, where they seem to possess greater proliferation capacity and differentiation potential than their adult counterparts Guillot et al 2007b;Pappa & Anagnou 2009 Gucciardo et al 2009), but express stromaassociated markers CD29 (b1-integrin), CD73 (SH3 and SH4), CD105 (SH2), CD44 (HCAM1), the early bone marrow progenitor cell marker CD90 (thy-1) and the extracellular matrix proteins vimentin, laminin and fibronectin (Guillot et al 2006(Guillot et al , 2007a. Contrary to adult bone marrow MSCs, first-trimester foetal blood, liver and bone marrow MSCs express baseline levels of the pluripotency stem cell markers Oct-4, Nanog, Rex-1, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 (Guillot et al 2007b;Zhang et al 2009).…”

Section: Foetal Tissuesmentioning

confidence: 99%

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Abdulrazzak

Moschidou

Jones

et al. 2010

J. R. Soc. Interface.

128

118

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Foetal stem cells (FSCs) can be isolated during gestation from many different tissues such as blood, liver and bone marrow as well as from a variety of extraembryonic tissues such as amniotic fluid and placenta. Strong evidence suggests that these cells differ on many biological aspects such as growth kinetics, morphology, immunophenotype, differentiation potential and engraftment capacity in vivo. Despite these differences, FSCs appear to be more primitive and have greater multi-potentiality than their adult counterparts. For example, foetal blood haemopoietic stem cells proliferate more rapidly than those found in cord blood or adult bone marrow. These features have led to FSCs being investigated for pre-and post-natal cell therapy and regenerative medicine applications. The cells have been used in pre-clinical studies to treat a wide range of diseases such as skeletal dysplasia, diaphragmatic hernia and respiratory failure, white matter damage, renal pathologies as well as cancers. Their intermediate state between adult and embryonic stem cells also makes them an ideal candidate for reprogramming to the pluripotent status.

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“…Advantages of amnion over other sources for stem cells included abundant availability, ethically non-objectionable and non-invasive source. The MSCs from AM (AMSCs) are thought to be in an intermediate stage between embryonic stem cells and lineage-restricted adult stem cells (In't Anker et al 2004;De Coppi et al 2007;Gucciardo et al 2009). Recently, Yamahara et al (2014) reported that mesenchymal stem cells isolated from human amniotic tissue have an angiogenesis potential.…”

Section: Introductionmentioning

confidence: 99%

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Shah

Yadav

2015

Cytotechnology

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The developmental ability and gene expression pattern at 8-to 16-cell and blastocyst stages of buffalo (Bubalus bubalis) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical ''S'' shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (OCT4, SOX2, NANOG), and mesenchymal stem cell markers (CD29, CD44) and were negative for haematopoietic marker (CD34) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3-4 passage were employed for NT. The cleavage rate was significantly (P \ 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (P \ 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (P [ 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (P \ 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), metabolism (GLUT1) and oxidative stress (MnSOD) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.

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Fetal mesenchymal stem cells: isolation, properties and potential use in perinatology and regenerative medicine

Cited by 89 publications

References 59 publications

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Isolation and differentiation potential of an equine amnion-derived stromal cell line

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

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