Isolation and differentiation potential of an equine amnion-derived stromal cell line

Violini, Stefania; Gorni, C.; Pisani, Laura Francesca; Ramelli, P.; Caniatti, M.; Mariani, Paola

doi:10.1007/s10616-011-9398-x

Cited by 16 publications

(9 citation statements)

References 28 publications

(32 reference statements)

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“…Many reports demonstrated that amnion-derived cells have potential to differentiate into cells of mesenchymal lineages in vitro in many species such as bovine , canine (Park et al 2012), feline (Rutigliano et al 2013), chicken (Gao et al 2012), rat (Marcus et al 2008) and equine (Coli et al 2011;Violini et al 2012;Seo et al 2013). In support to these reports, we successfully differentiate the buffalo mesenchymal stem cell-like cells derived from term placenta into adipogenic, chondrogenic and osteogenic cells of mesodermal lineage (Fig.…”

Section: Discussionsupporting

confidence: 78%

“…Recently, Yamahara et al (2014) reported that mesenchymal stem cells isolated from human amniotic tissue have an angiogenesis potential. In animals, amnion-derived stem cells have potential of differentiation into multilineage mesenchymal cell types in many species such as bovine , canine (Park et al 2012), feline (Rutigliano et al 2013), chicken (Gao et al 2012), rat (Marcus et al 2008) and equine (Coli et al 2011;Violini et al 2012;Seo et al 2013). This makes these cells more suitable candidates for cell therapy with less chances of immune-rejection (Lovati et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Ghosh

Kumar

Singh

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Ghosh

Kumar

Singh

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

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“…Trilineage differentiation potential has been described for equine MSCs derived from bone marrow (74), adipose tissue (97, 98), peripheral blood (99, 100), umbilical cord blood (101), umbilical cord matrix (82), gingiva and periodontal ligament (90), amnion (88) and amnion fluid (84), although the extent of differentiation varied based on the cell source and the differentiation protocols used. Adipogenic differentiation of equine MSCs strongly depends on the components of the differentiation media (102).…”

Section: In Vitro Characterization Of Equine Stem Cellsmentioning

confidence: 99%

Equine cellular therapy—from stall to bench to bedside?

et al. 2012

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Pioneering clinical stem cell research is being performed in the horse, a recipient of cutting edge veterinary medicine as well as a unique animal model, paving the way for human medical applications. Although demonstrable progress has been made on the clinical front, in vitro characterization of equine stem cells is still in comparatively early stages. To translate the promising results of clinical stem cell therapy in the horse, advances must be made in the characterization of equine stem cells. Aiming to improve communication between veterinarians and other natural scientists, this review gives an overview of veterinary ''bedside'' achievements, focusing on stem cell therapies in equine orthopedics as well as the current state of in vitro characterization of equine multipotent mesenchymal stromal cells (MSCs) and equine embryonic stem cells (ESCs). ' 2012 International Society for Advancement of Cytometry

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“…Recently, Yamahara et al (2014) reported that mesenchymal stem cells isolated from human amniotic tissue have an angiogenesis potential. In animals, amnion-derived stem cells have potential of differentiation into multilineage mesenchymal cell types in many species such as bovine , canine (Park et al 2012), feline (Rutigliano et al 2013), chicken (Gao et al 2012), rat (Marcus et al 2008) and equine (Coli et al 2011;Violini et al 2012;Seo et al 2013). Further, the amnion derived MSCs are reported to express certain key pluripotency markers including OCT4 .…”

Section: Introductionmentioning

confidence: 99%

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

Shah

Yadav

2015

Cytotechnology

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The developmental ability and gene expression pattern at 8-to 16-cell and blastocyst stages of buffalo (Bubalus bubalis) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical ''S'' shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (OCT4, SOX2, NANOG), and mesenchymal stem cell markers (CD29, CD44) and were negative for haematopoietic marker (CD34) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3-4 passage were employed for NT. The cleavage rate was significantly (P \ 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (P \ 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (P [ 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (P \ 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (OCT4, STAT3, NANOG), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), growth factor signaling and imprinting (IGF2, IGF2R), apoptosis (BAX, BCL2), metabolism (GLUT1) and oxidative stress (MnSOD) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.

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Isolation and differentiation potential of an equine amnion-derived stromal cell line

Cited by 16 publications

References 28 publications

Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Equine cellular therapy—from stall to bench to bedside?

Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells

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