2015
DOI: 10.1007/s10616-015-9936-z
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: The developmental ability and gene expression pattern at 8-to 16-cell and blastocyst stages of buffalo (Bubalus bubalis) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical ''S'' shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1

Citation Types

1
12
0

Year Published

2016
2016
2023
2023

Publication Types

Select...
4
4

Relationship

1
7

Authors

Journals

citations
Cited by 19 publications
(13 citation statements)
references
References 105 publications
1
12
0
Order By: Relevance
“…In several studies, mRNA expression levels of BAX have been used to evaluate embryo quality developed in vitro (Amarnath et al 2007;Mundim et al 2009;Shiqiang et al 2010;Kim et al 2012;Sadeesh et al 2015b). Data from the current study indicate that BAX expression was greater (P \ 0.05) in embryos produced from undefined media-containing serum than those produced in its absence, suggesting that over expression of the BAX gene, in the cellular apoptotic pathway takes place (Yang and Rajamahendran 2002;Opiela et al 2008).…”
Section: Discussionmentioning
confidence: 52%
“…In several studies, mRNA expression levels of BAX have been used to evaluate embryo quality developed in vitro (Amarnath et al 2007;Mundim et al 2009;Shiqiang et al 2010;Kim et al 2012;Sadeesh et al 2015b). Data from the current study indicate that BAX expression was greater (P \ 0.05) in embryos produced from undefined media-containing serum than those produced in its absence, suggesting that over expression of the BAX gene, in the cellular apoptotic pathway takes place (Yang and Rajamahendran 2002;Opiela et al 2008).…”
Section: Discussionmentioning
confidence: 52%
“…Messenger RNA for IGF-2R was already reported in bovine and buffalo embryos (Yaseen et al 2001;Pandey et al 2009;Sadeesh et al 2015Sadeesh et al , 2016. Cultured oocytes from in vitro showed fourfold fewer transcripts of IGF-2R in comparison with in vivo matured oocytes (Katz-Jaffe et al 2009).…”
Section: Discussionmentioning
confidence: 84%
“…These cells have been isolated and characterized from many tissues and animal species. Buffalo non-embryonic derived mesenchymal stem cells have been derived from amniotic fluid [ 7 ], bone marrow [ 11 ], umbilical cord matrix [ 34 ], adipose tissue [ 33 ] and amniotic membrane [ 14 , 15 , 24 , 31 ]. In the previous reports, there were many ambiguous results when defining the buffalo amniotic membrane derived cells, including the gestational stages, isolation methods, the identification of marker genes, the purity of the amniotic mesenchymal stem cells and the differentiation potential.…”
Section: Discussionmentioning
confidence: 99%
“…In buffalo, owing to its characteristics of heat resistance, high humidity resistance and strong disease resistance, it has tremendous potential development capabilities in agriculture across the globe. Only a few researches have reported the bAMSCs [ 7 , 14 , 15 , 24 , 31 ]. There are many controversies about these cells, such as the derivation of the AMSCs from different gestational stages, the different isolation methods used, the expression of specific markers to determine cell types and the ability of the cells to differentiate.…”
mentioning
confidence: 99%