Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol

Cryopreservation of common carp sperm

“…Most publications report the use of either Kurokura's Original Extender 1 and 2 (Kurokura et al, 1984, Lubzens et al, 1993, its modifications (Magyary et al, 1996) or other ionic solutions (Lahnsteiner et al, 2000). Sugar extenders were successfully used for the cryopreservation of sperm of several other fish species such as African catfish (Clarias gariepinus) (Steyn and Van Vuren, 1987;Urbányi et al, 1999) and various sturgeon species (Tsvetkova et al, 1996;Glogowski et al, 2002). The success of sugars as extenders can be explained by their role as external cryoprotectants and membrane stabilizers (Maisse, 1996).…”

New extender for cryopreservation of Siberian sturgeon (Acipenser baerii) semen

“…The composition of extenders for the cryopreservation of sturgeons sperm usually consists of 23.4-30.0 mM sucrose and 0.25-1.0 mM KCl buffered by Tris-HCl supplemented with cryoprotectants (5-10% of methanol, ME2SO or DMA) [5,12,16]. Our work indicates that after the reduction of components in the extender to glucose and methanol this procedure can be an alternative cryopreservation method to those previously described for sturgeons.…”

“…Two extenders were prepared: GM (0.1 M glucose in 15% methanol) and TSKM (30 mM Tris, 23.4 mM sucrose, 0.25 mM KCl in 10% methanol, pH 8.0) [16]. The post-thaw sperm motility parameters and fertilizing ability of sperm cryopreserved in TSKM and GM extenders were tested on three individual samples of sperm (n = 3).…”

“…Early studies on sturgeon sperm cryopreservation were carried out in the 1960s [6] with limited success. Since the 90s, methods resulting in sufficient sperm post-thaw motility have been described [16,25,30]. The latter methods employed extenders consisting of potassium ions and sucrose buffered by Tris-HCl in methanol.…”

Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%

“…Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196 C) sebagai cairan pembeku dengan cairan krioprotektan untuk melindungi sel spermanya. Saat ini ±200 spesies ikan di dunia yang berhasil diawetkan semennya menggunakan teknik kriopreservasi (Glogowski et al, 2002), diantaranya ikan mas (Cyprinus carpio) (Kurokura et al, 1984), lele Afrika (Clarias gariepenus) (Urbayi et al, 1999) dan ikan salmon Atlantik (Salmo salar) (Stoss dan Refstie, 1983).…”