Cryopreservation of common carp sperm

Horváth, Ákos; Miskolczi, Edit; Urbányi, Béla

doi:10.1016/s0990-7440(03)00084-6

Cited by 122 publications

(65 citation statements)

References 17 publications

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“…According to Ashwood-Smith (1980), methanol is in capability of penetrating to the cells with extreme rapidity. In addition, Horvath et al (2003) also reported highest fertilization and hatching rates in common carp eggs inseminated with sperm frozen with glucose and fructose based extenders that's containing methanol as internal permeable cryoprotectant.…”

Section: Discussionmentioning

confidence: 89%

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Bozkurt

Yavaş

2017

Journal of Limnology and Freshwater Fisheries Research

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Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straw volumes and thawing rates on the postthaw quality and fertilization ability of cryopreserved common carp (Cyprinus carpio) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl2, 1 mM MgSO4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4 °C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120 ºC) and finally stored in liquid nitrogen (-196 ºC) tank. Thawing of cryopreserved semen process was performed at 30 ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using a ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30 ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

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Section: Discussionmentioning

confidence: 89%

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Bozkurt

Yavaş

2017

Journal of Limnology and Freshwater Fisheries Research

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“…Significant inter-species variations as well as significant within-species differences in cryoprotectant effectiveness have been reported in fish spermatozoa (Alavi et al 2012). For example, 8-10% DMSO + P was associated with the highest sperm motility after freeze/thaw in tench ; MET was reported to be the optimal cryoprotectant for sperm of roach Rutilus rutilus, bream Abramis brama, silver bream Blicca bjoerkna, and barbel Barbus barbus (Urbanyi et al 2006); and DMSO has been recommended for common carp (Horvath et al 2003). The tested cryoprotectant treatments of tench GC showed no significant differences, indicating high stability of GC in cryoprotectants.…”

Section: Discussionmentioning

confidence: 99%

Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca)

Linhartová¹,

Rodina²,

Güralp³

et al. 2014

CZECH J ANIM SCI

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A practical technique for isolation and cryopreservation of tench (Tinca tinca) (Cyprinidae, Teleostei) early stages of germ cells (GC), including spermatogonia and spermatocytes, is reported for the first time. The germ-line cells possess the ability to differentiate into functional gametes of both sexes. These early stages of germ cells are small enough to be well-suited to cryopreservation, which, together with their high level of plasticity, makes their preservation a promising tool for maintaining genetic resources. Testicular cells were distinguished and separated by Percoll gradient, with the highest proportion of GC (62.2%) obtained from the 30% layer. The concentration and viability of GC were determined, and specific staining (DDX4) for germ cells was used to distinguish GC from somatic cells. Early stages of germ cells were cryopreserved in an extender composed of phosphate buffered saline (pH 8) with 0.5% BSA, 50mM d-glucose, and containing 1.5M cryoprotectant in the pre-programmed PLANER Kryo10 series III using a cooling protocol from +10°C to -80°C at a rate of 1°C/min. The effect of six cryoprotectants -methanol, dimethyl sulfoxide, dimethyl sulfoxide + propanediol (1 : 1), glycerol, ethylene glycol, and dimethylacetamid was assessed, and the results were evaluated by comparing the percentage of viable frozen/thawed GC by ANOVA, Tukey's HSD test (P < 0.05). Almost the same viability rates were obtained with no significant differences among tested cryoprotectants, indicating high stability of GC in cryoprotectants. Nevertheless, glycerol at a concentration of 1.5M was associated with the highest survival rate of thawed tench GC (57.69 ± 16.85%).

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“…Sperm (200 μl) mixed with a cryopreservation medium (200 μl cryoprotectant, 1600 μl diluent) in a ratio of 1:9 (Horváth et al, 2003;Urbányi et al, 2006) was pipetted into individually marked 0.5-ml straws after 3 minutes of equilibration time. Samples were cryopreserved in a polystyrene box filled with liquid nitrogen.…”

Section: First Experimentsmentioning

confidence: 99%

“…After the freezing process straws were placed into liquid nitrogen and stored there until being used. Thawing was carried out in a 40˚C water bath for 13 seconds (Horváth et al, 2003;Horváth et al, 2005). After thawing sperm motility was examined with the same method as described at fresh sperm.…”

Section: First Experimentsmentioning

confidence: 99%

Sperm Cryopreservation of Two European Predator Fish Species, the Pikeperch (Sander lucioperca) and the Wels Catfish (Silurus glanis)

Bokor¹,

Urbányi²,

Horváth³

et al. 2012

Current Frontiers in Cryopreservation

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Cryopreservation of common carp sperm

Cited by 122 publications

References 17 publications

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca)

Sperm Cryopreservation of Two European Predator Fish Species, the Pikeperch (Sander lucioperca) and the Wels Catfish (Silurus glanis)

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